The <i>Eucalyptus</i> linker histone variant EgH1.3 cooperates with the transcription factor EgMYB1 to control lignin biosynthesis during wood formation

Soler, Marçal; Plasencia, Anna; Larbat, Romain; Pouzet, Cécile; Jauneau, Alain; Rivas, Susana; Pesquet, Edouard; Lapierre, Catherine; Truchet, Isabelle; Grima‐Pettenati, Jacqueline

doi:10.1111/nph.14129

Cited by 47 publications

(35 citation statements)

References 54 publications

(89 reference statements)

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“…In this respect, we found that the upregulation in holm oak of the putative ortholog of Eucalyptus EgMYB1 and Arabidopsis FLC. EgMYB1 can interact with a linker histone and it has been hypothesized that it represses the phenylpropanoid biosynthetic enzymes through chromatin remodeling (Soler et al 2017). FLC (FLOWERING LOCUS C) is a floral repressor that integrates vernalization responses (Sheldon et al 2000) and in perennial trees an FLC-like gene has also been highlighted as a candidate for bud dormancy regulation (Porto et al 2015).…”

Section: Discussionmentioning

confidence: 99%

A comparative transcriptomic approach to understanding the formation of cork

et al. 2017

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Key message The transcriptome comparison of two oak species reveals possible candidates accounting for the exceptionally thick and pure cork oak phellem, such as those involved in secondary metabolism and phellogen activity. Abstract Cork oak, Quercus suber, differs from other Mediterranean oaks such as holm oak (Quercus ilex) by the thickness and organization of the external bark. While holm oak outer bark contains sequential periderms interspersed with dead secondary phloem (rhytidome), the cork oak outer bark only contains thick layers of phellem (cork rings) that accumulate until reaching a thickness that allows industrial uses. Here we compare the cork oak outer bark transcriptome with that of holm oak. Both transcriptomes present similitudes in their complexity, but whereas cork oak external bark is enriched with upregulated genes related to suberin, which is the main polymer responsible for the protective function of periderm, the upregulated categories of holm oak are enriched in abiotic stress and chromatin assembly. Concomitantly with the upregulation of suberin-related genes, there is also induction of regulatory and meristematic genes, whose predicted activities agree with the increased number of phellem layers found in the cork oak sample. Further transcript profiling among different cork oak tissues and conditions suggests that cork and wood share many regulatory mechanisms, probably reflecting similar ontogeny. Moreover, the analysis of transcripts accumulation during the cork growth season showed that most regulatory genes are upregulated early in the season when the cork cambium becomes active. Altogether our work provides the first transcriptome comparison between cork oak and holm oak outer bark, which unveils new regulatory candidate genes of phellem development.

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Section: Discussionmentioning

confidence: 99%

A comparative transcriptomic approach to understanding the formation of cork

et al. 2017

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“…Overexpression of Eucalyptus gunni EgMYB1 in thale cress and poplar negatively regulates SCW formation, including lignin biosynthesis [45]. When interacting with the linker histone variant EgH1.3, EgMYB1 displays stronger repressive activity, thereby preventing premature lignification of xylem cells during their early stages of differentiation [46]. The two orthologs of EgMYB1 in Populus deltoides and P. tomentosa (PdMYB221 and PtoMYB156) also reduce the SCW thickness of xylem fibers and the content of cellulose, lignin, and xylan [47,48].…”

Section: R2r3 Mybs the Gatekeepers Of Scw Formation And Lignificationmentioning

confidence: 99%

A Molecular Blueprint of Lignin Repression

Behr¹,

Guerriero²,

Grima‐Pettenati³

et al. 2019

Trends in Plant Science

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Although lignin is essential to ensure the correct growth and development of land plants, it may be an obstacle to the production of lignocellulosics-based biofuels, and reduces the nutritional quality of crops used for human consumption or livestock feed. The need to tailor the lignocellulosic biomass for more efficient biofuel production or for improved plant digestibility has fostered considerable advances in our understanding of the lignin biosynthetic pathway and its regulation. Most of the described regulators are transcriptional activators of lignin biosynthesis, but considerably less attention has been devoted to the repressors of this pathway. We provide a comprehensive overview of the molecular factors that negatively impact on the lignification process at both the transcriptional and post-transcriptional levels. Challenging LigninLignin is a major cell wall component that fulfills fundamental functions in plant development as well as in defense against pests and pathogens. This heteropolymer impregnates the compound middle lamella and the secondary cell walls (SCWs) of cells genetically programmed to be lignified, such as xylem tracheary elements as well as xylem and phloem fibers. Lignin biosynthesis spans the phenylpropanoid and the monolignol pathways, from phenylalanine to p-coumaryl, coniferyl, and sinapyl alcohols. Monolignol homeostasis is regulated through a balance between the molecular factors that antagonistically regulate their biosynthesis. After monolignols are excreted into the apoplast, they are oxidized by the phenol-oxidoreductase laccases and/or by class III peroxidases, and are polymerized by radical coupling. A complex regulatory network involving phytohormones, transcription factors (TFs), and post-transcriptional events guide SCW deposition and lignification [1]. This network prevents lignin deposition in tissues undergoing active division or elongation such as apical meristems, vascular cambium, and immature xylem cells, as well as in non-lignified tissues such as stem pith and flax or hemp bast fibers that harbor gelatinous walls under normal developmental conditions. Similarly, this network negatively regulates lignification in xylem tissue formed in response to mechanical stresses such as, for instance, poplar tension wood that harbors hypolignified gelatinous walls.

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“…During this developmental process, xylem initial cells produced by the meristematic vascular cambium undergo elongation, secondary cell wall (SCW) deposition, lignification and finally programmed cell death (in the case of fibres and vessels) in a narrow tissue layer within the stem 3, 4 . The process is likely to be extensively regulated at the chromatin level, as demonstrated for example through the regulation of lignification by histone H1.3 in Eucalyptus 5 . The majority of plant epigenomic studies, however, have been based on whole organs or complex mixtures of tissues that provide limited resolution of cell- or tissue-specific epigenetic regulation during development.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis and transcript abundance modelling of H3K4me3 and H3K27me3 in developing secondary xylem

Hussey

Loots

Merwe

et al. 2017

Sci Rep

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Despite the considerable contribution of xylem development (xylogenesis) to plant biomass accumulation, its epigenetic regulation is poorly understood. Furthermore, the relative contributions of histone modifications to transcriptional regulation is not well studied in plants. We investigated the biological relevance of H3K4me3 and H3K27me3 in secondary xylem development using ChIP-seq and their association with transcript levels among other histone modifications in woody and herbaceous models. In developing secondary xylem of the woody model Eucalyptus grandis, H3K4me3 and H3K27me3 genomic spans were distinctly associated with xylogenesis-related processes, with (late) lignification pathways enriched for putative bivalent domains, but not early secondary cell wall polysaccharide deposition. H3K27me3-occupied genes, of which 753 (~31%) are novel targets, were enriched for transcriptional regulation and flower development and had significant preferential expression in roots. Linear regression models of the ChIP-seq profiles predicted ~50% of transcript abundance measured with strand-specific RNA-seq, confirmed in a parallel analysis in Arabidopsis where integration of seven additional histone modifications each contributed smaller proportions of unique information to the predictive models. This study uncovers the biological importance of histone modification antagonism and genomic span in xylogenesis and quantifies for the first time the relative correlations of histone modifications with transcript abundance in plants.

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The Eucalyptus linker histone variant EgH1.3 cooperates with the transcription factor EgMYB1 to control lignin biosynthesis during wood formation

Cited by 47 publications

References 54 publications

A comparative transcriptomic approach to understanding the formation of cork

A comparative transcriptomic approach to understanding the formation of cork

A Molecular Blueprint of Lignin Repression

Integrated analysis and transcript abundance modelling of H3K4me3 and H3K27me3 in developing secondary xylem

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