“…Mutants for eight such genes have been characterized using various techniques. The genes identified and verified by gene disruption or replacement include the Tri5 gene of the trichothecene gene cluster encoding trichodiene synthase (Proctor et al, 1995), two MAP kinase genes, Mgv1 (Hou et al, 2002) and Map1, also called gpmk1 (Jenczmionka et al, 2003;Urban et al, 2003), a secreted lipase gene, Fgl1 (Voigt et al, 2005), a gene similar to Cps1 from Cochliobolus heterostrophus (Lu et al, 2003) and two polyketide synthase genes, Zea1 (PKS4) and Zea2 (PKS13), involved in the synthesis of the mycotoxin zearalenone (Gaffoor et al, 2005;Kim et al, 2005). Apart from these, genes encoding a predicted NADH : ubiquinone oxidoreductase, a putative b-ZIP transcription factor, a transducin b-subunit-like protein, as well as hydroxymethylglutaryl-CoA reductase (HMR1) and cystathionine beta-lyase (CBL1) have also been identified to be involved in pathogenicity through restriction-enzyme-mediated insertion (REMI) and directed mutagenesis (Seong et al, 2005(Seong et al, , 2006.…”