The <i>in vitro</i> Generation and Functional Analysis of Murine T Cell Populations and Clones Specific for a Protozoan Parasite, <i>Leishmania tropica</i>

Louis, Jacques; Zubler, Rudolf H.; Coutinho, S.; Lima, Gustavo R.; Behin, Reza; Mauël, Jacques; Engers, Howard

doi:10.1111/j.1600-065x.1982.tb00378.x

Cited by 55 publications

(18 citation statements)

References 70 publications

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“…However, limiting dilution experiments showed that the frequency of T cells reactive specifically with L. major antigens was surprisingly low (less than 1 in 500, [19]). This finding could result from an inability of APC to deliver in vitro the relevant antigens of a structure as complex as a parasite in a manner amenable to measuring the frequency of L. majorspecific T cells.…”

Section: Discussionmentioning

confidence: 97%

Vβ gene repertoires in T cells expanded in local self‐healing and lethal systemic murine cutaneous leishmaniasis

et al. 1994

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Inbred mice infected with Leishmania major promastigotes display two different courses of leishmaniasis: resistant strains develop self-healing local sores, while susceptible strains show progressive systemic disease with lethal outcome. Resistance predominantly correlates with the production of T helper type 1 (TH1) lymphokines and susceptibility with production of TH2-type lymphokines. Here, we analyzed whether this TH phenotype difference correlates with expression of particular T cell receptor V beta chains. Our results show that T cells expand strongly during infection in all groups of mice and invariantly express the same V beta gene families as prior to infection. Our data indicate that TH1 and TH2 cells use similar V beta gene families, and argue against the engagement of a restricted set of V beta by dominant determinants associated with L. major.

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Section: Discussionmentioning

confidence: 97%

Vβ gene repertoires in T cells expanded in local self‐healing and lethal systemic murine cutaneous leishmaniasis

et al. 1994

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“…Using L. major infection in the mouse, however, very few of the lymphocytes in immune mice expressed the Lyt-2 antigen marker of cytotoxic T lymphocytes. 18 Furthermore, the mouse/L. major model immune lymphocytes had no in vitro cytotoxic effect when incubated with syngeneic, L.-major-infected macrophages.…”

Section: Discussionmentioning

confidence: 99%

Intestinal Parasites: Incidence and Etiology in over 1,000 Patients at King Faisal Specialist Hospital in Riyadh

Qadri

Khalil

1987

Ann Saudi Med

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T-lymphocyte phenotypes and human leukocyte antigen-DR expression (HLA-DR) expression in skin lesions and draining lymph nodes of zoonotic leishmaniasis caused by Leishmania major were studied. T lymphocytes were the dominant cells in the lymphocytic preponderance type of lesion. They were lowest when the parasitized macrophage was the dominant cell or when the reaction consisted of an equal admixture of lymphocytes, macrophages and plasma cells. Both inducer/helper and suppressor/cytotoxic T cells were present in varying proportions. The latter were thought to be suppressor rather than cytotoxic cells. Their late appearance in the host reaction was believed to play a major role in halting the inflammatory response. Low levels of cells expressing the suppressor/cytotoxic phenotype and the continued presence of antigen in macrophages was associated with persistence of the lesions even when parasites were largely eliminated after specific antileishmanial therapy. Such lesions healed after local or systemic steroid therapy. The majority of cells in the dermal infiltrate were HLA-DR positive. Keratinocytes also expressed HLA-DR antigen.AM El-Hassan, R Kubba, YM Al-Gindan, AS Omer, MK Kutty, MBM Saeed, Lymphocyte

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“…If, however, one parasite succeeds in gaining entrance into a macrophage, infection may occur. In this case, the glycoconjugate displayed on the infected macrophage may induce a second level of immune attack by specific T cells, leading to macrophage activation and parasite killing (21)(22)(23)(24).…”

Section: Discussionmentioning

confidence: 99%

Immunization with Leishmania receptor for macrophages protects mice against cutaneous leishmaniasis.

Handman¹,

Mitchell²

1985

Proc. Natl. Acad. Sci. U.S.A.

121

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The Leishmania major receptor for macrophages is a lipid-containing glycoconjugate that is recognized by the monoclonal antibody WIC-79.3. When L. major promastigotes were incubated with Fab fragments of prior to injection into genetically susceptible mice, their infectivity was decreased. Fab fragments from an irrelevant control antibody of the same class had no effect. The L. major glycolipid was purified from detergent-solubilized promastigotes by affinity chromatography on immobilized WIC-79

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The in vitro Generation and Functional Analysis of Murine T Cell Populations and Clones Specific for a Protozoan Parasite, Leishmania tropica

Cited by 55 publications

References 70 publications

Vβ gene repertoires in T cells expanded in local self‐healing and lethal systemic murine cutaneous leishmaniasis

Vβ gene repertoires in T cells expanded in local self‐healing and lethal systemic murine cutaneous leishmaniasis

Intestinal Parasites: Incidence and Etiology in over 1,000 Patients at King Faisal Specialist Hospital in Riyadh

Immunization with Leishmania receptor for macrophages protects mice against cutaneous leishmaniasis.

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