With
the advancement in nanotechnology, particularly the use of
TiO2 nanoparticles (NPs), there is a need to study their
release into the environment and assess the related risk in an environmentally
relevant contamination scenario. In the present study, the transfer
and toxicity of TiO2 NPs in microcosms mimicking terrestrial
and aquatic ecosystems were evaluated. The contaminated soil was prepared
by spiking natural soils, with these then used as the basis for all
exposure systems including preparation of soil leachates for amphibian
exposure. Results demonstrated significant reductions in bacterial
(−45%) and archaeal (−36%) nitrifier abundance; significant
translocation of Ti to M. truncatula leaves (+422%); significant reductions in plant height (−17%),
number of leaves (−29%), and aboveground biomass (−53%);
nonsignificant Ti uptake in snail foot and viscera, and excretion
in feces; and genotoxicity to X. laevis larvae (+119% micronuclei). Our study highlights a possible risk
of engineered TiO2 NPs in the environment in terms of trophic
transfer and toxicity in both terrestrial and aquatic environments.