The miR-124 family of microRNAs is crucial for regeneration of the brain and visual system in the planarian Schmidtea mediterranea

Abstract

Brain regeneration in planarians is mediated by precise spatiotemporal control of gene expression and is crucial for multiple aspects of neurogenesis. However, the mechanisms underpinning the gene regulation essential for brain regeneration are largely unknown. Here, we investigated the role of the miR-124 family of microRNAs in planarian brain regeneration. The miR-124 family (miR-124) is highly conserved in animals and regulates neurogenesis by facilitating neural differentiation, yet its role in neural wiring and brain organization is not known. We developed a novel method for delivering anti-miRs using liposomes for the functional knockdown of microRNAs. Smed-miR-124 knockdown revealed a key role for these microRNAs in neuronal organization during planarian brain regeneration. Our results also demonstrated an essential role for miR-124 in the generation of eye progenitors. Additionally, miR-124 regulates Smed-slit-1, which encodes an axon guidance protein, either by targeting slit-1 mRNA or, potentially, by modulating the canonical Notch pathway. Together, our results reveal a role for miR-124 in regulating the regeneration of a functional brain and visual system.

MiR-124 and the Underlying Therapeutic Promise of Neurodegenerative Disorders

“…The efficiency of the combined miRNA uptake by the cell is also improved (Obeid et al, 2017;Tapeinos et al, 2017). Sasidharan et al (2017) developed a novel liposome-based method for delivering anti-miR-124 efficiently into live planarians to demonstrate the roles of the miR-124 family of miRs in regulating the regeneration of a functional brain. Poly lactic-co-glycolic acid (PLGA) is a Food and Drug Administration approved biocompatible polymer, which can be hydrolyzed into nontoxic lactic acid and glycolic acid monomers and metabolized by the human body without any side effects (Devulapally and Paulmurugan, 2014).…”

Comprehensive annotation and characterization of planarian tRNA and tRNA-derived fragments (tRFs)

“…Previous studies have comprehensively characterised the expression of several microRNAs (miRNAs) during planarian regeneration (16). Knockdown of miR-124 family of miRNAs, resulted in the mis-patterning of brain and central nervous system during regeneration (20). However, our understanding of small RNAs in planarians and regeneration are largely limited to miRNAs and piRNAs.…”

Avaliação Sensorial E Microbiológica Na Produção De Geleia Tipo Extra De Manga Com Cravo-Da-Índia

“…As proteínas são de grande importância para a qualidade do sorvete, pois influencia no batimento, emulsificação e melhorando a textura e estrutura (SILVEIRA et al, 2009 A metodologia do nocaute gênico por meio do RNA de interferência (RNAi) teve uma importância na elucidação da função dos 21 genes pesquisados -ovo, six -1, six-2, eya, otxA, sp6-9, dlx, soxB, meis, klf, foxQ2, arrestina, opsina, triptófano hidroxilase (tph) e tirosina hidroxilase (th) e os cingo genes (a, b, c, d e e) da família miR-124 3,5,9,22,23 . As médias e os respectivos desvio padrão e coeficiente de variação dos parâmetros físico-químicos foram obtidos com o auxílio do software Microsoft Office Excel (2015).…”