The <i>Mythimna separata</i> general odorant binding protein 2 (<scp>MsepGOBP2</scp>) is involved in the larval detection of the sex pheromone (<i>Z</i>)‐11‐hexadecenal

Tian, Zhen; Li, Ruichi; Cheng, Shichang; Zhou, Tong; Liu, Jiyuan

doi:10.1002/ps.7373

Cited by 5 publications

(7 citation statements)

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“…separata, and S. litura, GOBP2 has been confirmed as a key protein in recognizing respective sex pheromone molecules. ,, Some recent evidence show that GOBP2 is directly involved in the perception of a sex pheromone component, ( Z , E )-9, 11-tetradecadienyl acetate, in adult S. litura .…”

Section: Discussionmentioning

confidence: 98%

“…12 Similar phenomena have also been reported in other Lepidoptera pests like Mythimna separata, Spodoptera littoralis, and Spodoptera litura. 14,15,32 Thus, it is assumed that there may exist synergistic activity between the sex pheromone and certain HPVs. Determining the volatiles whose capacity to attract larvae can be enhanced by sex pheromones would be of significance to the development of larval olfactory behavior regulation.…”

Section: ■ Discussionmentioning

confidence: 99%

“…In the larvae of pests like P. xylostella, M. separata, and S. litura, GOBP2 has been confirmed as a key protein in recognizing respective sex pheromone molecules. 12,14,15 Some recent evidence show that GOBP2 is directly involved in the perception of a sex pheromone component, (Z, E)-9, 11-tetradecadienyl acetate, in adult S. litura. 14 Additionally, insect GOBP2 can bind to many other compounds with different structures, including insecticides, 42,46,47 suggesting it may have multiple unknown physiological functions that warrant further investigation.…”

Section: ■ Discussionmentioning

confidence: 99%

“…While regulating the behavior of adult pests can reduce the offspring population, it has a certain latency in pest control and cannot promptly reduce pest numbers. In recent years, research related to larval olfaction has gained attention. − For example, Di et al (2017) successfully designed an attractant for the larvae of Helicoverpa armigera based on the dose response of its larval olfactory receptors to host volatiles . However, research on the olfactory mechanisms and behavior regulation of P.…”

Section: Introductionmentioning

confidence: 99%

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Identifying the Key Role of Plutella xylostella General Odorant Binding Protein 2 in Perceiving a Larval Attractant, (E,E)-2,6-Farnesol

Wang,

Liu,

et al. 2024

J. Agric. Food Chem.

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There is currently a lack of effective olfaction-based techniques to control diamondback moth (DBM) larvae. Identifying behaviorally active odorants for DBM larvae and exploring their recognition mechanisms can provide insights into olfaction-based larval control strategies. Through the two-choice assay, (E,E)-2,6-farnesol (farnesol) was identified as a compound exhibiting significant attractant activity toward DBM larvae, achieving an attraction index of 0.48 ± 0.13. PxylGOBP1 and PxylGOBP2, highly expressed in the antennae of DBM larvae, both showed high affinity toward farnesol. RNAi technology was used to knock down PxylGOBP1 and PxylGOBP2, revealing that the attraction of DBM larvae to farnesol nearly vanished following the knockdown of PxylGOBP2, indicating its critical role in recognizing farnesol. Further investigation into the PxylGOBP2-farnesol interaction revealed the importance of residues like Thr9, Trp37, and Phe118 in PxylGOBP2’s binding to farnesol. This research is significant for unveiling the olfactory mechanisms of DBM larvae and developing larval behavior regulation techniques.

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Section: Discussionmentioning

confidence: 98%

Section: ■ Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identifying the Key Role of Plutella xylostella General Odorant Binding Protein 2 in Perceiving a Larval Attractant, (E,E)-2,6-Farnesol

Wang,

Liu,

et al. 2024

J. Agric. Food Chem.

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“…In recent years, numerous OBPs in insects had been found to express in both larva and adult ( Xue et al., 2016 ; Liu et al., 2020 ; Tian et al., 2023 ). In this study, our results showed that PverOBP18 gene expression was highest in the pupae, followed by the female adults, and lower in the 1st-3rd larvae and male adults with lowest in eggs.…”

Section: Discussionmentioning

confidence: 99%

Odorant binding protein 18 increases the pathogen resistance of the imported willow leaf beetle, Plagiodera versicolora

Rong,

He,

Liu

et al. 2024

Front. Cell. Infect. Microbiol.

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BackgroundInsect odorant-binding proteins (OBPs) are a class of small molecular weight soluble proteins. In the past few years, OBPs had been found to work as carriers of ligands and play a crucial role in olfaction and various other physiological processes, like immunity. A subset of insect OBPs had been found to be expressed differently and play a function in immunity of fungal infection. However, there are few studies on the role of OBPs in immunity of bacterial infection.MethodsTo identify the immune-related OBPs of Plagiodera versicolora after infected by Pseudomonas aeruginosa, we determined the mortality of P. versicolora to P. aeruginosa and selected the time point of 50% mortality of larvae to collect samples for RNA-seq. RNAi technology was used to investigate the function of immune-related OBPs after P. aeruginosa infection.ResultsRNA-seq data shows that PverOBP18 gene significantly up-regulated by 1.8-fold and further RT-qPCR affirmed its expression. Developmental expression profile showed that the expression of PverOBP18 was highest in the pupae, followed by the female adults, and lower in the 1st-3rd larvae and male adults with lowest in eggs. Tissue expression profiling showed that PverOBP18 was dominantly expressed in the epidermis. RNAi knockdown of PverOBP18 significantly reduced the expression of bacterial recognition receptor gene PGRP and antibacterial peptide gene Attacin and reduced the resistance of P. versicolora to P. aeruginosa infection.ConclusionOur results indicated that PverOBP18 gene increased the pathogen resistance of P. versicolora by cooperating with the immune genes and provided valuable insights into using OBPs as targets to design novel strategies for management of P. versicolora.

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An angiotensin converting enzyme homolog is required for volatile pheromone detection, odorant binding protein secretion and normal courtship behavior in Drosophila melanogaster

Sengupta

Powell

et al. 2023

Genetics

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In many arthropods, including insects responsible for transmission of human diseases, behaviors that include mating, aggregation and aggression are triggered by detection of pheromones. Extracellular odorant binding proteins are critical for pheromone detection in many insects and are secreted into the fluid bathing the olfactory neuron dendrites. In Drosophila melanogaster, the odorant binding protein LUSH is essential for normal sensitivity to the volatile sex pheromone, 11-cis vaccenyl acetate (cVA). Using a genetic screen for cVA pheromone insensitivity, we identified ANCE-3, a homolog of human angiotensin converting enzyme that is required for detection of cVA pheromone. The mutants have normal dose-response curves for food odors, although olfactory neuron amplitudes are reduced in all olfactory neurons examined. ance-3 mutants have profound delays in mating, and the courtship defects are primarily but not exclusively due to loss of ance-3 function in males. We demonstrate that ANCE-3 is required in the sensillae support cells for normal reproductive behavior, and that localization of odorant binding proteins to the sensillum lymph is blocked in the mutants. Expression of an ance-3 cDNA in sensillae support cells completely rescues the cVA responses, LUSH localization and courtship defects. We show the courtship latency defects are not due to effects on olfactory neurons in the antenna nor mediated through ORCO receptors, but instead stem from ANCE-3-dependent effects on chemosensory sensillae in other body parts. These findings reveal an unexpected factor critical for pheromone detection with profound influence on reproductive behaviors.

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The Mythimna separata general odorant binding protein 2 (MsepGOBP2) is involved in the larval detection of the sex pheromone (Z)‐11‐hexadecenal

Cited by 5 publications

References 78 publications

Identifying the Key Role of Plutella xylostella General Odorant Binding Protein 2 in Perceiving a Larval Attractant, (E,E)-2,6-Farnesol

Identifying the Key Role of Plutella xylostella General Odorant Binding Protein 2 in Perceiving a Larval Attractant, (E,E)-2,6-Farnesol

Odorant binding protein 18 increases the pathogen resistance of the imported willow leaf beetle, Plagiodera versicolora

An angiotensin converting enzyme homolog is required for volatile pheromone detection, odorant binding protein secretion and normal courtship behavior in Drosophila melanogaster

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