The peu‐miR160a−PeARF17.1/PeARF17.2 module participates in the adventitious root development of poplar

Liu, Sian; Yang, Chunxia; Wu, Ling; Cai, Heng; Li, Huogen; Xu, Meng

doi:10.1111/pbi.13211

Cited by 47 publications

(29 citation statements)

References 52 publications

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“…In Arabidopsis, AUXIN RESPONSE FACTOR ( ARF ) 6 , 8 and 17 antagonistically orchestrate AR formation and are negatively regulated by miR167 and miR160, respectively (Gutierrez et al ., 2009). Similarly, miR160‐ ARF17 regulates AR formation in Populus deltoides × Populus euramericana (Liu et al ., 2019). A miR156‐mediated SPL3 ‐ MADS50 module was recently shown to orchestrate AR formation in rice by affecting auxin signaling (Shao et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

The microRNA476a‐RFL module regulates adventitious root formation through a mitochondria‐dependent pathway in Populus

Tao

et al. 2021

New Phytologist

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For woody plants, clonal propagation efficiency is largely determined by adventitious root (AR) formation at the bases of stem cuttings. However, our understanding of the molecular mechanisms contributing to AR morphogenesis in trees remains limited, despite the importance of vegetative propagation, currently the most common practice for tree breeding and commercialization.Here, we identified Populus-specific miR476a as a regulator of wound-induced adventitious rooting that acts by orchestrating mitochondrial homeostasis.MiR476a exhibited inducible expression during AR formation and directly targeted several Restorer of Fertility like (RFL) genes encoding mitochondrion-localized pentatricopeptide repeat proteins. Genetic modification of miR476a-RFL expression revealed that miR476a/ RFL-mediated dynamic regulation of mitochondrial homeostasis influences AR formation in poplar. Mitochondrial perturbation via exogenous application of a chemical inhibitor indicated that miR476a/RFL-directed AR formation depends on mitochondrial regulation that acts via auxin signaling.Our results thus establish a microRNA-directed mitochondrion-auxin signaling cascade required for AR development, providing insights into the role of mitochondrial regulation in the developmental plasticity of plants.

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Section: Introductionmentioning

confidence: 99%

The microRNA476a‐RFL module regulates adventitious root formation through a mitochondria‐dependent pathway in Populus

Tao

et al. 2021

New Phytologist

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“…Adventitious root (AR) formation is crucial for plant growth and adaptation to various environmental conditions. The ability of AR formation is the most important requisite for vegetative propagated fruit and forestry species (Li et al 2017;Liu et al 2020;Rasmussen et al 2015). Exogenous and endogenous factors including phytohormones, environments, and plant age determine the AR formation (da Costa et al 2013;Dawood et al 2016;Druege et al 2019).…”

Section: Introductionmentioning

confidence: 99%

γ-Aminobutyric Acid Participates in the Adult-Phase Adventitious Rooting Recalcitrance

Shen

et al. 2020

J Plant Growth Regul

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In many tree species especially the rooting-recalcitrant woody perennials, the adventitious root (AR) in the juvenile phase can be easily induced by exogenous auxin, but AR formation becomes recalcitrant in the adult phase. Also, it is reported that the γ-aminobutyric acid (GABA) inhibits primary root growth in Arabidopsis and the AR formation in poplar (Populus ssp). So far, how GABA affects or is affected by the ontogenetic phase or auxin remains unclear. Here, we used an apple rootstock, Malus xiaojinensis, and tobacco (Nicotiana benthamiana) to investigate this question. We first analyzed the content of GABA, the activity of GABA synthetic enzyme GAD, and the expression of the coding gene MxGADs, respectively, in leafy cuttings of juvenile and adult phase. Next, the effect of exogenous GABA on AR formation was examined in in vitro shoots of M. xiaojinensis and tobacco. Interestingly, significant and consistent increases in GABA concentration, GAD activity, and expression of MxGAD genes in response to exogenous indole butyric acid (IBA) were detected in adult-phase cuttings, but not in juvenile-phase cuttings. Exogenous GABA application inhibited the AR formation by delaying rooting time and reducing root number and the total root length in in vitro shoots of both M. xiaojinensis and tobacco. The expression of MxPIN members increased in response to IBA application, but these changes were restrained by the addition of GABA. These results indicate that both the loss of juvenility and IBA are required to trigger GABA accumulation. GABA may affect the AR formation as a co-actor by inhibiting polar auxin transport. Together, these findings facilitate the understanding of the regulatory network among GABA, juvenility, and auxin signaling on the AR formation.

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“… Cai et al (2019) showed that, in poplar, the cuttings with overexpression of PeARF8.1 gene displayed more ARs than the wild type, and miR167a targets PeARF6s and PeARF8s to regulate AR formation. Similarly, Liu et al (2020) showed that the poplar peu-miR160a negatively regulated the genes PeARF10.1 , PeARF16.1 , PeARF16.2 , PeARF16.3 , PeARF17.1 , and PeARF17.2 . Overexpressing PeARF17.1 or mPeARF17.2 significantly increased AR number in the cuttings.…”

Section: Molecular Bases Associated With Auxin Signaling During Advenmentioning

confidence: 87%

Molecular Bases for the Regulation of Adventitious Root Generation in Plants

2021

Front. Plant Sci.

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The formation of adventitious roots (ARs) is an ecologically and economically important developmental process in plants. The evolution of AR systems is an important way for plants to cope with various environmental stresses. This review focuses on identified genes that have known to regulate the induction and initiation of ARs and offers an analysis of this process at the molecular level. The critical genes involved in adventitious rooting are the auxin signaling-responsive genes, including the AUXIN RESPONSE FACTOR (ARF) and the LATERAL ORGAN BOUNDARIES-DOMAIN (LOB) gene families, and genes associated with auxin transport and homeostasis, the quiescent center (QC) maintenance, and the root apical meristem (RAM) initiation. Several genes involved in cell wall modulation are also known to be involved in the regulation of adventitious rooting. Furthermore, the molecular processes that play roles in the ethylene, cytokinin, and jasmonic acid signaling pathways and their crosstalk modulate the generation of ARs. The crosstalk and interaction among many molecular processes generates complex networks that regulate AR generation.

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The peu‐miR160a−PeARF17.1/PeARF17.2 module participates in the adventitious root development of poplar

Cited by 47 publications

References 52 publications

The microRNA476a‐RFL module regulates adventitious root formation through a mitochondria‐dependent pathway in Populus

The microRNA476a‐RFL module regulates adventitious root formation through a mitochondria‐dependent pathway in Populus

γ-Aminobutyric Acid Participates in the Adult-Phase Adventitious Rooting Recalcitrance

Molecular Bases for the Regulation of Adventitious Root Generation in Plants

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