The
            <i>Pseudomonas aeruginosa</i>
            Universal Stress Protein PA4352 Is Essential for Surviving Anaerobic Energy Stress

Boes, Nelli; Schreiber, Kerstin; Härtig, Elisabeth; Jaensch, Lothar; Schobert, Max

doi:10.1128/jb.00308-06

Cited by 60 publications

(61 citation statements)

References 51 publications

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“…Category 2 comprised proteins involved in diverse physiological processes, ranging from metabolism, adaptation/protection, and attachment through to secreted factors. Furthermore, two Usp-type stress proteins, PA4352 and PA3309, which have been previously shown to be induced under anaerobic conditions (4,26), were highly expressed in biofilms and C40h. This could indicate an anaerobic environment in these growth modes.…”

Section: Vol 189 2007 Proteomics Of Colonies Biofilms and Planktomentioning

confidence: 85%

Interrelationships between Colonies, Biofilms, and Planktonic Cells of Pseudomonas aeruginosa

et al. 2007

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Pseudomonas aeruginosa is a gram-negative bacterium and an opportunistic human pathogen that causes chronic infections in immunocompromised individuals. These infections are hard to treat, partly due to the high intrinsic resistance of the bacterium to clinically used antibiotics and partly due to the formation of antibiotic-tolerant biofilms. The three most common ways of growing bacteria in vitro are as planktonic cultures, colonies on agar plates, and biofilms in continuous-flow systems. Biofilms are known to express genes different from those of planktonic cells, and biofilm cells are generally believed to closely resemble planktonic cells in stationary phase. However, few, if any, studies have examined global gene expression in colonies. We used a proteomic approach to investigate the interrelationships between planktonic cells, colonies, and biofilms under comparable conditions. Our results show that protein profiles in colonies resemble those of planktonic cells. Furthermore, contrary to what has been reported previously, the protein profiles of biofilms were found to more closely resemble those of exponentially growing planktonic cells than those of planktonic cells in the stationary phase. These findings raise some intriguing questions about the true nature of biofilms.

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Section: Vol 189 2007 Proteomics Of Colonies Biofilms and Planktomentioning

confidence: 85%

Interrelationships between Colonies, Biofilms, and Planktonic Cells of Pseudomonas aeruginosa

et al. 2007

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“…Transcription of the RSP0697 gene is probably under the control of FnrL, because there are two FNR-binding motifs in the promoter region between ccoN and the RSP0697 gene (82). RSP0697 is similar to PA4352 of Pseudomonas aeruginosa, which has recently been shown to be essential for survival under anaerobic energy stress conditions (9,73).…”

Section: Figmentioning

confidence: 99%

Transcriptome Dynamics during the Transition from Anaerobic Photosynthesis to Aerobic Respiration in Rhodobacter sphaeroides 2.4.1

2008

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Rhodobacter sphaeroides 2.4.1 is a facultative photosynthetic anaerobe that grows by anoxygenic photosynthesis under anaerobic-light conditions. Changes in energy generation pathways under photosynthetic and aerobic respiratory conditions are primarily controlled by oxygen tensions. In this study, we performed time series microarray analyses to investigate transcriptome dynamics during the transition from anaerobic photosynthesis to aerobic respiration. Major changes in gene expression profiles occurred in the initial 15 min after the shift from anaerobic-light to aerobic-dark conditions, with changes continuing to occur up to 4 hours postshift. Those genes whose expression levels changed significantly during the time series were grouped into three major classes by clustering analysis. Class I contained genes, such as that for the aa 3 cytochrome oxidase, whose expression levels increased after the shift. Class II contained genes, such as those for the photosynthetic apparatus and Calvin cycle enzymes, whose expression levels decreased after the shift. Class III contained genes whose expression levels temporarily increased during the time series. Many genes for metabolism and transport of carbohydrates or lipids were significantly induced early during the transition, suggesting that those endogenous compounds were initially utilized as carbon sources. Oxidation of those compounds might also be required for maintenance of redox homeostasis after exposure to oxygen. Genes for the repair of protein and sulfur groups and uptake of ferric iron were temporarily upregulated soon after the shift, suggesting they were involved in a response to oxidative stress. The flagellar-biosynthesis genes were expressed in a hierarchical manner at 15 to 60 min after the shift. Numerous transporters were induced at various time points, suggesting that the cellular composition went through significant changes during the transition from anaerobic photosynthesis to aerobic respiration. Analyses of these data make it clear that numerous regulatory activities come into play during the transition from one homeostatic state to another.

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“…The numbers of usp genes in Gram-negative bacteria are diverse, ranging from 1 to 12 (3,6,18,30,34), indicating that some functional redundancies of Usps exist or that each Usp might have a different role. We characterized the 11 usp genes identified in B. glumae BGR1.…”

Section: Discussionmentioning

confidence: 99%

“…UspC, UspF, and UspG are involved mainly in motility and adhesion (31). In Pseudomonas aeruginosa, Usps are essential for survival under conditions of anaerobic energy stress and are required for pyruvate fermentation (3,38).…”

mentioning

confidence: 99%

Regulation of Universal Stress Protein Genes by Quorum Sensing and RpoS in Burkholderia glumae

Kim

Goo

Kang

et al. 2011

Journal of Bacteriology

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Burkholderia glumae possesses a quorum-sensing (QS) system mediated by N-octanoyl-homoserine lactone (C 8 -HSL) and its cognate receptor TofR. TofR/C 8 -HSL regulates the expression of a transcriptional regulator, qsmR. We identified one of the universal stress proteins (Usps), Usp2, from a genome-wide analysis of QS-dependent proteomes of B. glumae. In the whole genome of B. glumae BGR1, 11 usp genes (usp1 to usp11) were identified. Among the stress conditions tested, usp1 and usp2 mutants died 1 h after heat shock stress, whereas the other usp mutants and the wild-type strain survived for more than 3 h at 45°C. The expressions of all usp genes were positively regulated by QS, directly by QsmR. In addition, the expressions of usp1 and usp2 were dependent on RpoS in the stationary phase, as confirmed by the direct binding of RpoS-RNA holoenzyme to the promoter regions of the usp1 and usp2 genes. The expression of usp1 was upregulated upon a temperature shift from 37°C to either 28°C or 45°C, whereas the expression of usp2 was independent of temperature stress. This indicates that the regulation of usp1 and usp2 expression is different from what is known about Escherichia coli. Compared to the diverse roles of Usps in E. coli, Usps in B. glumae are dedicated to heat shock stress.

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The Pseudomonas aeruginosa Universal Stress Protein PA4352 Is Essential for Surviving Anaerobic Energy Stress

Cited by 60 publications

References 51 publications

Interrelationships between Colonies, Biofilms, and Planktonic Cells of Pseudomonas aeruginosa

Interrelationships between Colonies, Biofilms, and Planktonic Cells of Pseudomonas aeruginosa

Transcriptome Dynamics during the Transition from Anaerobic Photosynthesis to Aerobic Respiration in Rhodobacter sphaeroides 2.4.1

Regulation of Universal Stress Protein Genes by Quorum Sensing and RpoS in Burkholderia glumae

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