The <i>Ralstonia solanacearum</i> csp22 peptide, but not flagellin‐derived peptides, is perceived by plants from the <i>Solanaceae</i> family

Wei, Yali; Cáceres-Moreno, Carlos; Jiménez-Gόngora, Tamara; Wang, Keke; Sang, Yuying; Lozano‐Durán, Rosa; Macho, Alberto P.

doi:10.1111/pbi.12874

Cited by 68 publications

(59 citation statements)

References 50 publications

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“…Our bacterial colonization experiments demon-strate that cellular damage and lateral root emergence does not only unlock MAMP responsiveness to high doses of pure MAMPs such as flg22, or 3-OH-C10:0, but is also effective in unlocking responses to the more complex and probably much less concentrated cocktail of MAMPs associated with actual bacterial colonization. Interestingly, flg22 derived from GMI1000 flagellin was found not to activate the Arabidopsis FLS2 receptor (Pfund et al, 2004;Wei et al, 2018). This indicates that the damage-associated MAMP responses we observe upon GMI1000 infection must be caused by MAMPs other than flg22.…”

Section: Local Gating Of Immune Responses By Damage In Root-bacteria mentioning

confidence: 80%

Co-incidence of Damage and Microbial Patterns Controls Localized Immune Responses in Roots

Zhou

Emonet

Tendon

et al. 2020

Cell

222

200

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Section: Local Gating Of Immune Responses By Damage In Root-bacteria mentioning

confidence: 80%

Co-incidence of Damage and Microbial Patterns Controls Localized Immune Responses in Roots

Zhou

Emonet

Tendon

et al. 2020

Cell

222

200

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“…This result is in agreement with a parallel study by Wei et al . (), who also identified csp22 as an R. solanacearum PAMP in strain GMI1000, which corresponds to allele 1 (Fig. ).…”

Section: List Of the Core Genes Of The 37 Ralstonia Solanacearum Stramentioning

confidence: 79%

“…2) two csp22 peptides were recognized as PAMPs in either A. thaliana or aubergine, as expected. This result is in agreement with a parallel study by Wei et al (2017), who also identified csp22 as an R. solanacearum PAMP in strain GMI1000, which corresponds to allele 1 (Fig. 1).…”

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confidence: 99%

“…Allele 5 includes eight strains (RS2, CPPB909, UW551, UY031, IPO1609, CFIA906, CPPB282 and POPS2). PAMP-encoding gene EF-Tu, but also the PAMP csp22-encoding gene Csp, which was identified in an independent and parallel study by Wei et al (2017). A class of efflux pumps with known roles in plant-microbe interactions was also flagged, suggesting that Tajima's D can generally identify genes with important roles in plant-microbe interactions.…”

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confidence: 99%

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The population genetic test Tajima's D identifies genes encoding pathogen‐associated molecular patterns and other virulence‐related genes in Ralstonia solanacearum

Eckshtain‐Levi

Weisberg

Vinatzer

2018

Molecular Plant Pathology

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The detection of pathogen-associated molecular patterns (PAMPs) by plant pattern recognition receptors (PRRs) is an essential part of plant immunity. Until recently, elf18, an epitope of elongation factor-Tu (EF-Tu), was the sole confirmed PAMP of Ralstonia solanacearum, the causal agent of bacterial wilt disease, limiting our understanding of R. solanacearum-plant interactions. Therefore, we set out to identify additional R. solanacearum PAMPs based on the hypothesis that genes encoding PAMPs are under selection to avoid recognition by plant PRRs. We calculated Tajima's D, a population genetic test statistic which identifies genes that do not evolve neutrally, for 3003 genes conserved in 37 R. solanacearum genomes. The screen flagged 49 non-neutrally evolving genes, including not only EF-Tu but also the gene for Cold Shock Protein C, which encodes the PAMP csp22. Importantly, an R. solanacearum allele of this PAMP was recently identified in a parallel independent study. Genes coding for efflux pumps, some with known roles in virulence, were also flagged by Tajima's D. We conclude that Tajima's D is a straightforward test to identify genes encoding PAMPs and other virulence-related genes in plant pathogen genomes.

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“…However, the control of bacterial wilt is difficult and ineffective due to the high genetic variability, persistence in the environment and broad host range of R. solanacearum. Thus, to discover alternative antimicrobial agents is urgent need [3,8].…”

mentioning

confidence: 99%

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Chen

Wang²,

Liu

et al. 2020

Preprint

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Background: There is an urgent need to discover alternative Bacillus biocontrol agents to control bacterial wilt. This study reports on a new lipopeptide-producing biocontrol strain FJAT-46737 and explores its lipopeptidic compounds, and this study investigates the antagonistic effects of these compounds.Results: Based on a whole genome sequence analysis, the new strain FJAT-46737 was identified as Bacillus velezensis, and seven gene clusters responsible for the synthesis of bioactive secondary metabolites in FJAT-46737 were predicted. The antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against three bacteria and three fungi. Pot experiments showed that the control efficiencies for tomato bacterial wilt of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was the secretion of lipopeptides consisting of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities against several pathogens (including Ralstonia solanacearum, Escherichia coli and Fusarium oxysporum) and fengycins were the major antibacterial components of the lipopeptides against R. solanacearum in vitro. Furthermore, the rich organic nitrogen sources (especially yeast extracts) in the media promoted the production of fengycin and surfactin by FJAT-46737. The secretion of these two lipopeptides was related to temperature fluctuations, with the fengycin content decreasing by 96.6% and the surfactins content increasing by 59.9% from 20 oC to 40 oC. The optimal temperature for lipopeptide production by FJAT-46737 varied between 20 oC and 25oC. Conclusions: The B. velezensis strain FJAT-46737 and its secreted lipopeptides could be used as new sources of potential biocontrol agents against several plant pathogens, and especially the bacterial wilt pathogen R. solanacearum.

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The Ralstonia solanacearum csp22 peptide, but not flagellin‐derived peptides, is perceived by plants from the Solanaceae family

Cited by 68 publications

References 50 publications

Co-incidence of Damage and Microbial Patterns Controls Localized Immune Responses in Roots

Co-incidence of Damage and Microbial Patterns Controls Localized Immune Responses in Roots

The population genetic test Tajima's D identifies genes encoding pathogen‐associated molecular patterns and other virulence‐related genes in Ralstonia solanacearum

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

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