The Saccharomyces cerevisiae TAN1 gene is required for N4-acetylcytidine formation in tRNA

Johansson, Marcus; Byström, Andeŕs S.

doi:10.1261/rna.5198204

Cited by 85 publications

(116 citation statements)

References 43 publications

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“…The screen for mutations lethal in combination with the sup61-T47:2C allele, encoding a species with an alteration in the variable arm (Figure 1(a)), identified mutants representing 12 complementation groups [11]. One group consisted of strains in which the mutation was genetically linked to the sup61 locus and this group was excluded from further analysis.…”

Section: Resultsmentioning

confidence: 99%

“…The mutant gene in each of the 11 remaining groups (Table 1) was identified by complementing the phenotype with a yeast genomic library and subsequent confirmation that the original mutation was genetically linked to the locus for the complementing gene. As the sup61-T47:2C allele generates a requirement for several different tRNA modifying enzymes [5,11], we expected that the screen would identify factors involved in the modification of . In addition to trm1, tan1 and bud13 mutants, the screen identified strains with mutations in the DUS2 and MOD5 genes (Table 1) [11,12,19].…”

Section: Resultsmentioning

confidence: 99%

“…As the sup61-T47:2C allele generates a requirement for several different tRNA modifying enzymes [5,11], we expected that the screen would identify factors involved in the modification of . In addition to trm1, tan1 and bud13 mutants, the screen identified strains with mutations in the DUS2 and MOD5 genes (Table 1) [11,12,19]. These genes encode the tRNA modifying enzymes that catalyze the formation of dihydrouridine (D) at position 20 and N 6 -isopentenyladenosine (i 6 A) at position 37, respectively (Figure 1(a)) [20–23].…”

Section: Resultsmentioning

confidence: 99%

“…To identify novel factors promoting tRNA maturation, we performed a screen for mutations lethal in combination with one of the four sup61 alleles [11]. The screen identified mutants representing 12 different complementation groups and detailed analyzes of one group led to the identification of TAN1 as a gene required for the formation of N 4 -acetylcytidine (ac 4 C) in tRNA [11].…”

Section: Introductionmentioning

confidence: 99%

“…The screen identified mutants representing 12 different complementation groups and detailed analyzes of one group led to the identification of TAN1 as a gene required for the formation of N 4 -acetylcytidine (ac 4 C) in tRNA [11]. The ac 4 C nucleoside is present at position 12 in leucine and serine isoacceptors, and the inactivation of TAN1 causes a lack of ac 4 C in tRNA and a reduction in the abundance of the altered [11]. Another complementation group consisted of a strain with a mutation in the BUD13 gene [12].…”

Section: Introductionmentioning

confidence: 99%

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Identification of factors that promote biogenesis of tRNA_CGA^Ser

Zhou

Byström

et al. 2018

RNA Biology

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A wide variety of factors are required for the conversion of pre-tRNA molecules into the mature tRNAs that function in translation. To identify factors influencing tRNA biogenesis, we previously performed a screen for strains carrying mutations that induce lethality when combined with a sup61-T47:2C allele, encoding a mutant form of . Analyzes of two complementation groups led to the identification of Tan1 as a protein involved in formation of the modified nucleoside N4-acetylcytidine (ac4C) in tRNA and Bud13 as a factor controlling the levels of ac4C by promoting TAN1 pre-mRNA splicing. Here, we describe the remaining complementation groups and show that they include strains with mutations in genes for known tRNA biogenesis factors that modify (DUS2, MOD5 and TRM1), transport (LOS1), or aminoacylate (SES1) . Other strains carried mutations in genes for factors involved in rRNA/mRNA synthesis (RPA49, RRN3 and MOT1) or magnesium uptake (ALR1). We show that mutations in not only DUS2, LOS1 and SES1 but also in RPA49, RRN3 and MOT1 cause a reduction in the levels of the altered . These results indicate that Rpa49, Rrn3 and Mot1 directly or indirectly influence biogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of factors that promote biogenesis of tRNA_CGA^Ser

Zhou

Byström

et al. 2018

RNA Biology

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Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting

Bryson

Gamage

Meier

2020

CP Chemical Biology

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Cytidine acetyltransferases are an emerging class of nucleic‐acid‐modifying enzymes responsible for the establishment of N4‐acetylcytidine (ac4C) in RNA. In contrast to histone acetyltransferases, whose activity is commonly studied by western blotting, relatively few methods exist for quickly assessing the activity of cytidine acetyltransferases from a biological sample of interest or the distribution of ac4C across different RNA species. In this protocol, we describe a method for analysis of cellular cytidine acetyltransferase activity using dot‐ and immuno‐northern‐blotting‐based detection. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Detection of N4‐Acetylcytidine in RNA by dot blotting Basic Protocol 2: Visualizing N4‐Acetylcytidine Distribution in RNA by northern blotting

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N‐acetyltransferase 10 facilitates tumorigenesis of diffuse large B‐cell lymphoma by regulating AMPK/mTOR signalling through N4‐acetylcytidine modification of SLC30A9

Ding,

Yu,

et al. 2024

Clinical & Translational Med

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BackgroundAccumulating studies suggested that posttranscriptional modifications exert a vital role in the tumorigenesis of diffuse large B‐cell lymphoma (DLBCL). N4‐acetylcytidine (ac4C) modification, catalyzed by the N‐acetyltransferase 10 (NAT10), was a novel type of chemical modification that improves translation efficiency and mRNA stability.MethodsGEO databases and clinical samples were used to explore the expression and clinical value of NAT10 in DLBCL. CRISPER/Cas9‐mediated knockout of NAT10 was performed to determine the biological functions of NAT10 in DLBCL. RNA sequencing, acetylated RNA immunoprecipitation sequencing (acRIP‐seq), LC‐MS/MS, RNA immunoprecipitation (RIP)‐qPCR and RNA stability assays were performed to explore the mechanism by which NAT10 contributed to DLBCL progression.ResultsHere, we demonstrated that NAT10‐mediated ac4C modification regulated the occurrence and progression of DLBCL. Dysregulated N‐acetyltransferases expression was found in DLBCL samples. High expression of NAT10 was associated with poor prognosis of DLBCL patients. Deletion of NAT10 expression inhibited cell proliferation and induced G0/G1 phase arrest. Furthermore, knockout of NAT10 increased the sensitivity of DLBCL cells to ibrutinib. AcRIP‐seq identified solute carrier family 30 member 9 (SLC30A9) as a downstream target of NAT10 in DLBCL. NAT10 regulated the mRNA stability of SLC30A9 in an ac4C‐dependent manner. Genetic silencing of SLC30A9 suppressed DLBCL cell growth via regulating the activation of AMP‐activated protein kinase (AMPK) pathway.ConclusionCollectively, these findings highlighted the essential role of ac4C RNA modification mediated by NAT10 in DLBCL, and provided insights into novel epigenetic‐based therapeutic strategies.

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The Saccharomyces cerevisiae TAN1 gene is required for N⁴-acetylcytidine formation in tRNA

Cited by 85 publications

References 43 publications

Identification of factors that promote biogenesis of tRNA_CGA^Ser

Identification of factors that promote biogenesis of tRNA_CGA^Ser

Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting

N‐acetyltransferase 10 facilitates tumorigenesis of diffuse large B‐cell lymphoma by regulating AMPK/mTOR signalling through N4‐acetylcytidine modification of SLC30A9

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The Saccharomyces cerevisiae TAN1 gene is required for N4-acetylcytidine formation in tRNA

Cited by 85 publications

References 43 publications

Identification of factors that promote biogenesis of tRNACGASer

Identification of factors that promote biogenesis of tRNACGASer

Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting

N‐acetyltransferase 10 facilitates tumorigenesis of diffuse large B‐cell lymphoma by regulating AMPK/mTOR signalling through N4‐acetylcytidine modification of SLC30A9

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The Saccharomyces cerevisiae TAN1 gene is required for N⁴-acetylcytidine formation in tRNA

Identification of factors that promote biogenesis of tRNA_CGA^Ser

Identification of factors that promote biogenesis of tRNA_CGA^Ser