The <i><scp>C</scp>ronobacter</i> sp. in milk and dairy products: Detection and typing

Ye, Yingwang; Li, Hui; Wu, Qingping; Zhang, Jumei; Lu, Yudong

doi:10.1111/1471-0307.12111

Cited by 18 publications

(16 citation statements)

References 60 publications

(83 reference statements)

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“…Although the main source of C. sakazakii is considered to be infant formula, this is still unclear (Ye et al . ). Investigations of different sources may contribute to better understanding of C. sakazakii as a pathogen.…”

Section: Discussionmentioning

confidence: 97%

“…This pathogen has been isolated from various sources including dairy products, vegetables, dried meats and water (Yan et al 2012;Vojkovska et al 2016). Although the main source of C. sakazakii is considered to be infant formula, this is still unclear (Ye et al 2014). Investigations of different sources may contribute to better understanding of C. sakazakii as a pathogen.…”

Section: Discussionmentioning

confidence: 99%

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Development and application of a new multiplex real‐time PCR assay for simultaneous identification of Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes in raw milk and cheese

Tutar

Akıncı

Akyol

2018

Int J of Dairy Tech

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Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes are important foodborne pathogens in milk and milk products, which are responsible for a variety of diseases that pose serious hazards to public health and food safety. The objective of this study was to develop a novel multiplex RTi‐PCR for the detection of B. melitensis, C. sakazakii and L. monocytogenes and to characterise the potential risk of these pathogens in raw milk and cheese. The raw milk (n = 25) and cheese samples (n = 20) were analysed by multiplex RTi‐PCR assay and detected for quantification of the three pathogens. In this study, B. melitensis, C. sakazakii and L. monocytogenes were simultaneously identified using BMEII0466, mms operon and hly as target genes, respectively. The multiplex RTi‐PCR assay that was developed showed good sensitivity and selectivity for the pathogenic microorganisms (r2 = 0.986–0.997). Multiplex RTi‐PCR results showed that most of the samples were contaminated with the pathogens screened.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Development and application of a new multiplex real‐time PCR assay for simultaneous identification of Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes in raw milk and cheese

Tutar

Akıncı

Akyol

2018

Int J of Dairy Tech

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“…; Ye et al . ). The International Commission for Microbiological Specification for Foods has ranked Cronobacter ( Enterobacter sakazakii ) as a “severe hazard for restricted populations, life threatening or substantial chronic sequelae of long duration.” The virulence difference among Cronobacter species were reported previously (Pagotto et al .…”

Section: Introductionmentioning

confidence: 97%

Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism Typing

Ling

Han

et al. 2015

Journal of Food Safety

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Cronobacter species are foodborne pathogens associated with neonatal meningitis, sepsis and necrotizing enterocolitis through consumption of contaminated powdered milk. However, there are no specific targets used for differentiating Cronobacter species. The DNA-based assay and polyphasic analysis have facilitated the detection and typing of Cronobacter species in foodborne outbreaks. In this study, polymerase chain reaction (PCR) and dot hybridization for detecting Cronobacter spp. was developed targeting gluB gene, then one PCR-restriction fragment length polymorphism (RFLP) typing assay with TaqI digestion was applied for four species differentiation within Cronobacter. Results indicated that the gluB gene is a specific target for detecting Cronobacter and the PCR-RFLP typing assay could conveniently and rapidly differentiate Cronobacter malonaticus, C. dublinensis, C. sakazakii and C. muytjensii. PRACTICAL APPLICATIONSIn this study, polymerase chain reaction (PCR) and dot hybridization targeting gluB for detecting Cronobacter was developed and a PCR-restriction fragment length polymorphism (RFLP) typing assay with TaqI was successfully applied for differentiating four Cronobacter species. Results indicated that gluB is a specific marker for detection of Cronobacter and PCR-RFLP targeting gluB gene product digested with TaqI could conveniently differentiate four Cronobacter species.

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“…C. sakazakii have associated with severe forms of necrotizing and meningitis especially in neonates with mortality rate varies from 40–80% (Bar‐Oz, Preminger, Peleg, Block, & Arad, ; Healy et al, ; Van Acker et al, ) and the infective dose is estimated to range from 10 3 to ≥10 8 cells (Iversen & Forsythe, ; Pagotto, Nazarowec‐White, Bidawid, & Farber, ). C. sakazakii has been isolated from wide range of dairy products (Ye, Li, Wu, Zhang, & Lu, ). Moreover, powder infant formula has been epidemiologically linked to Cronobacter infections in infants (Healy et al, ; Jaradat et al, ).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of camel milk on Cronobacter sakazakii

Abusheliabi

Al‐Rumaithi

Olaimat

et al. 2017

Journal of Food Safety

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The objective of this study was to investigate the behavior of Cronobacter sakazakii in raw and pasteurized camel milk compared with bovine milk at different incubation temperatures. Pasteurized and raw camel milk was inoculated with a cocktail of two strains of C. sakazakii and incubated at 10°C, 25°C, and 37°C to be sampled at 0, 2, 4, 8, and 24 hr. Pasteurized bovine milk was treated similarly and used as a control. Raw and pasteurized camel milk showed more inhibitory effect against C. sakazakii at 25°C than 37°C while there was no effect at 10°C. The inhibition ranged from 0.40 to 1.42 CFU/ml and 0.61 to 1.6 CFU/ml at 25°C, and from 0.40 to 0.92 CFU/ml and 0.40 to 0.80 CFU/ml at 37°C in pasteurized and raw camel milk after 24 hr, respectively, compared with bovine milk. Moreover, the inhibitory effect of pasteurized camel milk against C. sakazakii was more than raw camel milk after 24 hr at the different incubation temperatures which may approve that the natural antimicrobial proteins in camel milk were resistant to pasteurization temperature. Practical application Due to the association of Cronobacter sakazakii with powdered infant formula (PIF), controlling methods, therefore, are highly demanded by PIF manufacturers. The inhibitory activities of camel milk against C. sakazakii and nutritional value make camel milk an outstanding candidate to substitute bovine milk as a base of PIF.

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The Cronobacter sp. in milk and dairy products: Detection and typing

Cited by 18 publications

References 60 publications

Development and application of a new multiplex real‐time PCR assay for simultaneous identification of Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes in raw milk and cheese

Development and application of a new multiplex real‐time PCR assay for simultaneous identification of Brucella melitensis, Cronobacter sakazakii and Listeria monocytogenes in raw milk and cheese

Detection of Cronobacter on gluB Gene and Differentiation of Four Cronobacter Species by Polymerase Chain Reaction‐Restriction Fragment Length Polymorphism Typing

Inhibitory effect of camel milk on Cronobacter sakazakii

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