The <i>Sulfolobus solfataricus</i> AAA protein Sso0909, a homologue of the eukaryotic ESCRT Vps4 ATPase

Hobel, Cédric F.V.; Albers, Sonja‐Verena; Driessen, Arnold J. M.; Lupas, Andrei N.

doi:10.1042/bst0360094

Cited by 28 publications

(34 citation statements)

References 26 publications

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“…In addition, the operon is down-regulated during transition from exponential growth into stationary phase (our laboratory, unpublished), in accordance with a reduced need for division-related gene products. Further, and in agreement with an essential cellular role, deletion mutants in the S. solfataricus ortholog of the Saci_1372 gene (cdvC) could not be isolated in a previous study (8).…”

Section: Identification Of Genes Involved In Genome Segregation or Cellmentioning

confidence: 93%

“…CdvC is the archaeal ortholog of another eukaryotic type E sorting protein, Vps4 (8), an AAA ϩ -type ATPase involved in ATP-mediated disassembly of the ESCRT-III complex (10). Recently, the structural basis for selective recognition of eukaryotic ESCRT-III proteins by Vps4 was elucidated by showing that the N-terminal MIT domain of Vps4 recognizes and binds to a motif located at the C terminus of some of the ESCRT-III proteins (9,11).…”

Section: Identification Of Genes Involved In Genome Segregation or Cellmentioning

confidence: 99%

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A unique cell division machinery in the Archaea

Lindås

Karlsson

Lindgren

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

303

307

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In contrast to the cell division machineries of bacteria, euryarchaea, and eukaryotes, no division components have been identified in the second main archaeal phylum, Crenarchaeota. Here, we demonstrate that a three-gene operon, cdv, in the crenarchaeon Sulfolobus acidocaldarius, forms part of a unique cell division machinery. The operon is induced at the onset of genome segregation and division, and the Cdv proteins then polymerize between segregating nucleoids and persist throughout cell division, forming a successively smaller structure during constriction. The cdv operon is dramatically down-regulated after UV irradiation, indicating division inhibition in response to DNA damage, reminiscent of eukaryotic checkpoint systems. The cdv genes exhibit a complementary phylogenetic range relative to FtsZ-based archaeal division systems such that, in most archaeal lineages, either one or the other system is present. Two of the Cdv proteins, CdvB and CdvC, display homology to components of the eukaryotic ESCRT-III sorting complex involved in budding of luminal vesicles and HIV-1 virion release, suggesting mechanistic similarities and a common evolutionary origin.he Archaea constitute a separate domain of life that has evolved in parallel with Bacteria and Eukarya (1). The archaeal domain is currently divided into two main lineages, the Crenarchaeota and the Euryarchaeota, each of which comprises several distinct classes of organisms that thrive in a wide variety of environments. Whereas several aspects of archaeal biology appear to be unique, certain traits resemble those in eukaryotes, including the machineries that govern information storage, maintenance, and processing.Several features of archaeal cell cycle progression have been elucidated in considerable detail including the overall organization of the cell cycle in certain species, and regulatory and mechanistic aspects of the replication process (2, 3). Conversely, the genome segregation machinery remains essentially uncharacterized in this domain. In archaeal species belonging to the Euryarchaeota phylum, and in bacteria, cell division is mediated by FtsZ protein filaments that form a constricting ring structure (4). In eukaryotes, division occurs with the help of a contractile actin-myosin ring or, in plant cells, by septum formation at a site initially marked by actin and microtubules (5). In contrast to bacteria, euryarchaea, and eukaryotes, no cell division components have been identified in the second main archaeal phylum, Crenarchaeota (2).Here, we report on the identification of key components of the cell division system in the hyperthermophilic crenarchaeon Sulfolobus acidocaldarius, describe intracellular structures that are formed by the gene products during genome segregation and division, and show that the operon is subject to a checkpoint-like regulation. We also demonstrate that the division machinery is present in all crenarchaeal orders except Thermoproteales, and that it is related to the eukaryotic ESCRT-III sorting complex.

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Section: Identification Of Genes Involved In Genome Segregation or Cellmentioning

confidence: 93%

Section: Identification Of Genes Involved In Genome Segregation or Cellmentioning

confidence: 99%

A unique cell division machinery in the Archaea

Lindås

Karlsson

Lindgren

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

303

307

View full text Add to dashboard Cite

show abstract

“…Sequence analysis revealed that the ATPases contain an N-terminal microtubule interacting and trafficking (MIT) sub-domain and a putative C-terminal Vps4-oligomerization domain. As both domains are typically found in eukaryotic Vps4 proteins (StuchellBrereton et al 2007;Vajjhala et al 2006), the identified ATPases likely represent an archaeal ortholog of the eukaryotic Vps4 as was suggested recently (Hobel et al 2008;Obita et al 2007). In eukaryotes, Vps4 releases ESCRT-III sub-units from the endosomal membrane in an ATP-dependent manner (Babst et al 1998).…”

Section: Proteomic Analysis Of Extracellular Membrane Vesiclesmentioning

confidence: 99%

Proteomic analysis of secreted membrane vesicles of archaeal Sulfolobus species reveals the presence of endosome sorting complex components

et al. 2008

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The crenarchaea Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii, release membrane vesicles into the medium. These membrane vesicles consist of tetraether lipids and are coated with an S-layer. A proteomic analysis reveals the presence of proteins homologous to subunits of the eukaryotic endosomal sorting complex required for transport (ESCRT). Immunodetection of one of these homologs suggest a cell surface localization in intact cells. These data suggest that the membrane vesicles in Sulfolobus sp. emerge from a specific budding process with similarity to the endosomal sorting pathway.

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“…However, this view is countered by recent discoveries in Archaea, which is a separate domain of life that lacks endomembrane structures and is separated from humans by two-billion years of evolution. Remarkably, homologues of ESCRT-III proteins and VPS4 have been identified in Sulfolobus, a member of the Kingdom Crenarchaea (Hobel et al, 2008;Obita et al, 2007). Functional experiments have demonstrated that cell-cycle regulation and mid-…”

Section: Escrt Function At the Midbodymentioning

confidence: 99%

No strings attached: the ESCRT machinery in viral budding and cytokinesis

McDonald

Martín-Serrano

2009

Journal of Cell Science

104

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Since the initial discovery of the endosomal sorting complex required for transport (ESCRT) pathway, research in this field has exploded. ESCRT proteins are part of the endosomal trafficking system and play a crucial role in the biogenesis of multivesicular bodies by functioning in the formation of vesicles that bud away from the cytoplasm. Subsequently, a surprising role for ESCRT proteins was defined in the budding step of some enveloped retroviruses, including HIV-1. ESCRT proteins are also employed in this outward budding process, which results in the resolution of a membranous tether between the host cell and the budding virus particle. Remarkably, it has recently been described that ESCRT proteins also have a role in the topologically equivalent process of cell division. In the same way that viral particles recruit the ESCRT proteins to the site of viral budding, ESCRT proteins are also recruited to the midbody – the site of release of daughter cell from mother cell during cytokinesis. In this Commentary, we describe recent advances in the understanding of ESCRT proteins and how they act to mediate these diverse processes.

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The Sulfolobus solfataricus AAA protein Sso0909, a homologue of the eukaryotic ESCRT Vps4 ATPase

Cited by 28 publications

References 26 publications

A unique cell division machinery in the Archaea

A unique cell division machinery in the Archaea

Proteomic analysis of secreted membrane vesicles of archaeal Sulfolobus species reveals the presence of endosome sorting complex components

No strings attached: the ESCRT machinery in viral budding and cytokinesis

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