The <i>unfulfilled/DHR51</i> gene of <i>Drosophila melanogaster</i> modulates wing expansion and fertility

Sung, Carl; Wong, Laura E.; Sen, Lauren Q. Chang; Nguyen, Elizabeth T.; Lazaga, Nelson; Ganzer, Gavin; McNabb, Susan L.; Robinow, Steven

doi:10.1002/dvdy.21817

Cited by 22 publications

(33 citation statements)

References 62 publications

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“…Previously, unf was shown to be essential for the wing expansion and fertility of adult flies, and abundantly expressed in the developing MBs [49]. Here we studied the function of unf in further detail and learned that unf acts in all three major types of MB neurons to promote proper neuron subtype identity and axon guidance.…”

Section: Discussionmentioning

confidence: 90%

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Nuclear Receptor Unfulfilled Regulates Axonal Guidance and Cell Identity of Drosophila Mushroom Body Neurons

2009

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Nuclear receptors (NRs) comprise a family of ligand-regulated transcription factors that control diverse critical biological processes including various aspects of brain development. Eighteen NR genes exist in the Drosophila genome. To explore their roles in brain development, we knocked down individual NRs through the development of the mushroom bodies (MBs) by targeted RNAi. Besides recapitulating the known MB phenotypes for three NRs, we found that unfulfilled (unf), an ortholog of human photoreceptor specific nuclear receptor (PNR), regulates axonal morphogenesis and neuronal subtype identity. The adult MBs develop through remodeling of γ neurons plus de-novo elaboration of both α′/β′ and α/β neurons. Notably, unf is largely dispensable for the initial elaboration of γ neurons, but plays an essential role in their re-extension of axons after pruning during early metamorphosis. The subsequently derived MB neuron types also require unf for extension of axons beyond the terminus of the pruned bundle. Tracing single axons revealed misrouting rather than simple truncation. Further, silencing unf in single-cell clones elicited misguidance of axons in otherwise unperturbed MBs. Such axon guidance defects may occur as MB neurons partially lose their subtype identity, as evidenced by suppression of various MB subtype markers in unf knockdown MBs. In sum, unf governs axonal morphogenesis of multiple MB neuron types, possibly through regulating neuronal subtype identity.

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Section: Discussionmentioning

confidence: 90%

“…In addition to EcR , usp , and tll , we isolated unfulfilled ( unf ), the fly NR2E3 subfamily ortholog of C. elegans fax-1 and human photoreceptor-specific nuclear receptor ( PNR ) [48], [49]. unf has a novel function in MB development, and unf knockdown caused a severe MB lobe extension defect.…”

Section: Introductionmentioning

confidence: 99%

Nuclear Receptor Unfulfilled Regulates Axonal Guidance and Cell Identity of Drosophila Mushroom Body Neurons

2009

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“…The unfulfilled ( HR51 ) gene was named on the basis of the mutant phenotype in which eclosed adults fail to unfold their wings and exhibit reduced fertility [167,168]. In mushroom bodies, it functions in specifying neuron identities and regulating axon pathfinding [169].…”

Section: Unf/fax-1/pnr (Nr2e3)mentioning

confidence: 99%

Conserved and Exapted Functions of Nuclear Receptors in Animal Development

Bodofsky¹,

Koitz²,

Wightman³

2017

Nuclear Receptor Research

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The nuclear receptor gene family includes 18 members that are broadly conserved among multiple disparate animal phyla, indicating that they trace their evolutionary origins to the time at which animal life arose. Typical nuclear receptors contain two major domains: a DNA-binding domain and a C-terminal domain that may bind a lipophilic hormone. Many of these nuclear receptors play varied roles in animal development, including coordination of life cycle events and cellular differentiation. The well-studied genetic model systems of Drosophila, C. elegans, and mouse permit an evaluation of the extent to which nuclear receptor function in development is conserved or exapted (repurposed) over animal evolution. While there are some specific examples of conserved functions and pathways, there are many clear examples of exaptation. Overall, the evolutionary theme of exaptation appears to be favored over strict functional conservation. Despite strong conservation of DNA-binding domain sequences and activity, the nuclear receptors prove to be highly-flexible regulators of animal development.

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“…Unfortunately, the information about these genes is scarce. Recently, however, two independent DHR51 alleles have been characterized in D. melanogaster [158]. Consistent with the highly restricted expression of DHR51 in mushroom body neurons and in a small number of other cells of the central nervous system, DHR51 mutants displayed behavioural and sensory defects, including compromised fertility and coordination and wing expansion failure [158].…”

Section: Hr51 (Nr2e3) Hr83 (Nr2e5) and Nr2e6mentioning

confidence: 97%

“…Recently, however, two independent DHR51 alleles have been characterized in D. melanogaster [158]. Consistent with the highly restricted expression of DHR51 in mushroom body neurons and in a small number of other cells of the central nervous system, DHR51 mutants displayed behavioural and sensory defects, including compromised fertility and coordination and wing expansion failure [158]. Furthermore, it has been shown that DHR51 is a thiolate heme-binding protein and, as E75, binds NO and CO which suggests that this factor can also be a gas or a heme sensor [159].…”

Section: Hr51 (Nr2e3) Hr83 (Nr2e5) and Nr2e6mentioning

confidence: 99%

Functions of Nuclear Receptors in Insect Development

MartÍn

2010

Nuclear Receptors

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Abstract:Nuclear hormone receptors form a large family of ligand-activated transcription factors that regulate the transcription of target genes involved in a variety of important biological processes in animal development. Intense research over the past years has led to the characterization of the complete set of nuclear hormone receptors in several invertebrate genomes, including those of six insect species. In these organisms, extensive studies have defined critical roles for nuclear hormone receptors in controlling embryonic development, molting, metamorphosis, reproduction and metabolism. In this chapter, I review our understanding of the biological functions of all insect nuclear hormone receptors.

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The unfulfilled/DHR51 gene of Drosophila melanogaster modulates wing expansion and fertility

Cited by 22 publications

References 62 publications

Nuclear Receptor Unfulfilled Regulates Axonal Guidance and Cell Identity of Drosophila Mushroom Body Neurons

Nuclear Receptor Unfulfilled Regulates Axonal Guidance and Cell Identity of Drosophila Mushroom Body Neurons

Conserved and Exapted Functions of Nuclear Receptors in Animal Development

Functions of Nuclear Receptors in Insect Development

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