The ideal solvent for paper chromatography of food dyes

Markow, Peter G.

doi:10.1021/ed065p899

Cited by 7 publications

(4 citation statements)

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“…Simple paper chromatography experiments were performed using 7.5 × 13.5-cm pieces of Whatman No. 1 chromatography paper and 7 mL of 0.10 wt % aqueous table salt as the solvent in 250-mL beakers covered with Petri dish or watch glass covers . The food dyes were applied to the chromatography paper using wooden toothpicks bought at a grocery store.…”

Section: Paper Chromatography Of Synthetic Food Dyesmentioning

confidence: 99%

“…The separation and identification of these dyes using paper chromatography has been widely used by science teachers and chemistry instructors for many years because the experiments are simple, safe, and inexpensive. Because many students consume foods containing these dyes on a daily basis, they are personally connected and interested in the topic − . This Journal has also published works involving other forms of chromatography for dye separation and identification, including thin layer , high performance liquid , and column chromatography , .…”

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confidence: 99%

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A Global Perspective on the History, Use, and Identification of Synthetic Food Dyes

2010

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Section: Paper Chromatography Of Synthetic Food Dyesmentioning

confidence: 99%

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confidence: 99%

A Global Perspective on the History, Use, and Identification of Synthetic Food Dyes

2010

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“…Paper chromatography experiments are often used in introductory laboratory courses as the experiments can engage students to understand concepts of polarity in addition to basic chromatographic principles. Common experimental applications include the separation of food dyes, − indicator dyes, amino acids with ninhydrin visualization, , and tomato extracts . In addition, thin layer chromatography (TLC) laboratory experiments have also focused on separation of food dyes, plant pigments, inks, and other organic compounds. − The pairing of chemical concepts with tangible real-world examples helps motivate students to accomplish meaningful learning …”

Section: Introductionmentioning

confidence: 99%

Analysis and Identification of Major Organic Acids in Wine and Fruit Juices by Paper Chromatography

2018

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A simple and inexpensive paper chromatography experiment that separates and identifies major organic acids in wine and fruit juices has been developed for introductory organic undergraduate students to teach the basics of chromatography. This experiment reinforces several concepts for students such as compound separation via extraction and chromatography, intermolecular forces and acidity, and a comparison of organic acid polarities related to structure. In addition, the separation of acids within wine and/or fruit juices enhances student understanding of real-world organic acids present in foods. Citric, malic, tartaric, and lactic acids, all potential components of fruit juices and wine samples, are the focus of this experiment that includes a description of the malolactic fermentation occurring in wine samples. A mobile phase mixture of n-butanol, formic acid, acetone, and ethanol along with bromocresol green indicator allows for fast separation and visualization of the acids of interest. Retention factor calculation and identification of acid types present in selected samples encourage student understanding of overall acidity and the relationship of pK a values to acid structure. This analysis helped students understand the impact of intermolecular forces, pH, and the application of paper chromatography in real-world situations.

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“…Paper chromatography of food dyes is a popular experiment in many beginning laboratories at the university and high school level. Several articles in this Journal involve the separation and identification of food dyes (1)(2)(3)(4). In particular, paper chromatography was used to confirm the presence of yellow dye #5 in M&Ms (5).…”

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confidence: 99%