The identification of a B-cell epitope in bovine viral diarrhea virus (BVDV) core protein based on a mimotope obtained from a phage-displayed peptide library

Chen, Xinye; Ding, Xuezhi; Zhu, Liqian; Zhang, Gaiping

doi:10.1016/j.ijbiomac.2021.06.013

Cited by 5 publications

(2 citation statements)

References 60 publications

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“…The first step in creating porous membranes to capture specific mAbs is immobilization of a selective affinity molecule in the pores. Mimotope peptides are attractive for binding mAbs because they are relatively small molecules, easy to immobilize, and highly specific. − In prior work, we immobilized 25 ± 4 mg of the mimotope Tra19 per mL of a spongy nylon membrane . The immobilization method includes adsorption of a PAA-containing film, activation of the −COOH groups of PAA, and reaction of primary amines on the mimotope, Tra19, with active esters.…”

Section: Resultsmentioning

confidence: 99%

Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates

et al. 2022

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Quantitation of therapeutic monoclonal antibodies (mAbs) in human serum could ensure that patients have adequate levels of mAbs for effective treatment. This research describes the use of affinity, glass-fiber membranes in a 96-well-plate format for rapid (<5 min) quantitation of the therapeutic mAb trastuzumab and a mAb against the SARS-CoV-2 spike protein. Adsorption of a poly(acrylic acid)-containing film in membrane pores and activation of the −COOH groups in the film enable covalent-linking of affinity peptides or proteins to the membrane. Passage of mAb-containing serum through the affinity membrane results in mAb capture within 1 min. Subsequent rinsing, binding of a secondary antibody conjugated to a fluorophore, and a second rinse yield mAb-concentration-dependent fluorescence intensities in the wells. Calibration curves established from analyses on different days have low variability and allow determination of mAb levels in separately prepared samples with an average error <10%, although errors in single-replicate measurements may reach 40%. The assays can occur in diluted serum with physiologically relevant mAb concentrations, as well as in undiluted serum. Thus, the combination of 96-well plates containing affinity membranes, a microplate reader, and a simple vacuum manifold affords convenient mAb quantitation in <5 min.

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Section: Resultsmentioning

confidence: 99%

Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates

et al. 2022

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“…At present, studies on antigen epitopes by the phage display technique have been reported in many fields. For example, the epitopes and/or mimotopes for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [ 8 , 10 , 11 ], Mycoplasma pneumoniae [ 12 ], hepatitis C virus [ 13 ], human immunodeficiency virus [ 13 ], major capsid protein L1 of human papillomavirus types 18 and 45 (HPV18/45) [ 14 ], bovine viral diarrhea virus (BVDV) [ 15 ], influenza virus H1N1 [ 16 ], RhD antigen [ 17 ], influenza virus [ 18 ], norovirus [ 19 ] and etc. have been obtained successfully by using specific antibodies to screen phage display library combined with gene sequencing.…”

Section: Introductionmentioning

confidence: 99%

Screening of sperm antigen epitopes by phage display technique and its preliminary clinical application

Lü

Ge²,

Xu³

et al. 2022

Basic Clin. Androl.

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Background At present, there is a lack of standardized preparation methods of sperm antigen for the detection of antisperm antibody (AsAb). To screen sperm antigen mimotopes from a phage display random peptide library and use them to establish an enzyme-linked immunosorbent assay (ELISA) for the detection of AsAb, immunoglobulins were extracted from the sera of rabbits with positive AsAb and negative AsAb, respectively, by the saturated ammonium sulfate method, and a phage display 12-mer peptide library was affinity panned by the extracted immunoglobins coated on the ELISA plate. Then, the obtained positive phage clones were identified by ELISA and sent for sequencing and peptides synthesis. Last, a diagnostic ELISA was established to detect clinical serum and seminal plasma samples. Results A total of sixty phage clones were chosen by affinity panning, and sixteen of them reacted positively with AsAb in indirect ELISA and sandwich ELISA. Following DNA sequencing and translation, the peptide sequences of the sixteen positive clones were obtained. By comparison in Blast database, four of sixteen positive clones were found to be closely related to male reproduction. Two (#1 and #25) of four mimotopes were synthesized, and an ELISA method was established using the two mimotopes as sperm specific antigens. One hundred and thirty-four serum samples and seventy-four seminal plasma samples from infertile couples were analyzed by the established ELISA with #1 and #25 mimotopes, respectively. The positive rates of AsAb in serum samples were 20.15% (27/134) for #1 and 11.19% (15/134) for #25, respectively, and the coincidence rate between them was 91.04% (122/134). The positive rates of AsAb in seminal plasma samples were 1.35% (1/74) for both #1 and #25, and the coincidence rate was 100%. Conclusion Sperm antigen mimotopes can be obtained successfully by the phage display technique, and can be used as standard sperm specific antigens to establish an ELISA method for the detection of AsAb.

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Reverse vaccinology 2.0: computational resources for B-cell epitope prediction

Mishra,

Pandya,

Bhatt

et al. 2024

Reverse Vaccinology

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The identification of a B-cell epitope in bovine viral diarrhea virus (BVDV) core protein based on a mimotope obtained from a phage-displayed peptide library

Cited by 5 publications

References 60 publications

Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates

Quantitation of Trastuzumab and an Antibody to SARS-CoV-2 in Minutes Using Affinity Membranes in 96-Well Plates

Screening of sperm antigen epitopes by phage display technique and its preliminary clinical application

Reverse vaccinology 2.0: computational resources for B-cell epitope prediction

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