The Identification of Carbonyl Compounds by Use of 2,4-Dinitrophenylhydrazine

Abstract

In connection with another investigation under way in this Laboratory, use of the common reagents for a carbonyl group failed to give a solid derivative. At about the same time it was noted that there was no reliable rapid reagent for preparing solid derivatives of most of the aliphatic aldehydes and ketones. As a rule the reagents that form crystalline products with aromatic carbonyl compounds give oils with most of the corresponding aliphatic substances; in spite of this, Laboratory manuals in illustrating

Toluene photocatalytic oxidation at ppbv levels: Kinetic investigation and carbon balance determination

“…Thus, 1000 mL of the reaction chamber gas mixture was sampled in parallel using 2,4-dinitrophenylhydrazine (DNPH) Waters cartridges in order to perform carbonyl derivatization [21]. Those cartridges were eluted and analyzed by high-performance liquid chromatography (HPLC/UV).…”

The effect of solvent selection in the gas chromatographic analysis of carbonyls in air samples after derivatization with pentafluorophenyl hydrazine

“…The solubility of PFPH was not an issue in those studies because its concentrations were kept below the saturation point. In comparison, Allen (1930) used hot water, hydrazine sulfate, and potassium acetate to prepare 2,4-DNPH solution for the identification of carbonyls. Pang et al (2011) adopted a solvent-free method to form the CC hydrazones on the PFPH coated sorbent from which the hydrazones were extracted subsequently using hexane.…”

Determinação de compostos carbonilados e carboxilados em derivados de petróleo

“…6,8,26,[31][32][33][34][35] Os principais problemas desta técnica estão associados ao longo tempo para execução do procedimento, uso de reagentes, pré-concentração de impurezas dos reagentes e alta demanda de mão-de-obra por parte do analista.…”

“…(2) A clivagem da ligação ab, que não é observada, daria um íon de baixa estabilidade com dois centros positivos adjacentes (3) Quando R é C3 ou maior, o íon inicialmente formado pode quebrar novamente com rearranjo de hidrogênio: (4) Vol. 35,No. 8 Aldeídos alifáticos 56 O pico do íon molecular dos aldeídos alifáticos é, em geral, significativo.…”

“…6,8,26,[31][32][33][34][35][36]43,57,58 Para amostras de nafta, o procedimento mais simples envolve a extração líquido-líquido ou microextração em fase sólida dos aldeídos, cetonas e ácidos carboxílicos presentes. Em se tratando de amostras gasosas, um procedimento mais laborioso torna-se necessário com a adsorção dos oxigenados sobre um cartucho impregnado com agentes derivatizantes.…”