This article aimed to examine the impact of subjective norm (SN), attitude and perceived behavioural control (PBC) on Nigerian youths’ agro-entrepreneurial intention (EI) as well as moderating role of gender. The unit of analysis is 400 youths in Nigeria, ranging from ages of 18 to 40 years. In this study, structural equation modelling was used. The result shows that attitude, SN and PBC have significant relationship with Nigerian youths’ agro-EI. Also, there is no significant difference between men and women on PBC and attitude towards agro-entrepreneurship, but there is a significant difference on SN. Standard estimates revealed that women have a stronger SN than men. Finally, it was discovered that Nigerian youths have interest in agro-entrepreneurship. This study is unique since it focused specifically on agro-entrepreneurial intent and the moderating role of gender in an agrarian and gender sensitive zone, which many studies ignored. Gender role was emphasized in this study. Thus, it has shown that society and community have more influence on women than men when engaging in agro-entrepreneurship.