The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family

Zhu, Xiaoyuan; Shen, Chen; Jiao, Yang; Zhou, Shungui; Zeng, Liexian; Han, Jingluan; Su, Jing; Wang, Ling; Pan, Qinghua

doi:10.1007/s00122-012-1787-9

Cited by 77 publications

(52 citation statements)

References 39 publications

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“…Pathogen inoculation and disease evaluation were performed as previously described by Zhu et al (2012). The BC 3 F 3 homozygous lines of QTL1-C and QTL11-C were inoculated with 42 representative Philippine blast isolates.…”

Section: Methodsmentioning

confidence: 99%

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Chaipanya

Telebanco‐Yanoria

Quime

et al. 2017

Rice

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BackgroundRice (Oryza sativa) is one of the most important food crops in the world. Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. To effectively cope with this problem, the use of rice blast resistance varieties through innovative breeding programs is the best strategy to date. The Thai rice variety Jao Hom Nin (JHN) showed broad-spectrum resistance against Thai rice blast isolates. Two QTLs for blast resistance in JHN were reported on chromosome 1 (QTL1) and 11 (QTL11).ResultsMonogenic lines of QTL1 (QTL1-C) and QTL11 (QTL11-C) in the CO39 genetic background were generated. Cluster analysis based on the disease reaction pattern of QTL1-C and QTL11-C, together with IRBLs, showed that those two monogenic lines were clustered with IRBLsh-S (Pish) and IRBL7-M (Pi7), respectively. Moreover, sequence analysis revealed that Pish and Pi7 were embedded within the QTL1 and QTL11 delimited genomic intervals, respectively. This study thus concluded that QTL1 and QTL11 could encode alleles of Pish and Pi7, designated as Pish-J and Pi7-J, respectively. To validate this hypothesis, the genomic regions of Pish-J and Pi7-J were cloned and sequenced. Protein sequence comparison revealed that Pish-J and Pi7-J were identical to Pish and Pi7, respectively. The holistic disease spectrum of JHN was found to be exactly attributed to the additive ones of both QTL1-C and QTL11-C.ConclusionJHN showed broad spectrum resistance against Thai and Philippine rice blast isolates. As this study demonstrated, the combination of two resistance genes, Pish-J and Pi7-J, in JHN, with each controlling broad-spectrum resistance to rice blast disease, explains the high level of resistance. Thus, the combination of Pish and Pi7 can provide a practical scheme for breeding durable resistance in rice against rice blast disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-017-0159-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Chaipanya

Telebanco‐Yanoria

Quime

et al. 2017

Rice

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“…The physical locations of these genes are on the same locus, but express different levels of resistance spectra in different cultivars [26]. [106,151,152]. Allele mining for Pi-kh, Pi-ta and Pi-zt genes has been also reported in Indian land races of rice [153].…”

Section: Allele Mining Strategies For Blast Resistance Genesmentioning

confidence: 98%

“…The monitoring of an individual resistance gene and its pyramiding into a breeding line is very difficult; only a DNA marker tightly linked with the gene provides the straightforward way for the selection of multiple blast resistance genes. The selection of the desired traits can be made at an early stage with the advent of molecular markers [106]. When a molecular marker is found very close to the gene of interest, it acts as a tag to be used for indirect selection in the molecular breeding program [25].…”

Section: Identification Of Molecular Markers Linked To Blast Resistanmentioning

confidence: 99%

Current advance methods for the identification of blast resistance genes in rice

Tanweer

Rafii

Sijam

et al. 2015

Comptes Rendus. Biologies

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Rice blast caused by Magnaporthe oryzae is one of the most devastating diseases of rice around the world and crop losses due to blast are considerably high. Many blast resistant rice varieties have been developed by classical plant breeding and adopted by farmers in various rice-growing countries. However, the variability in the pathogenicity of the blast fungus according to environment made blast disease a major concern for farmers, which remains a threat to the rice industry. With the utilization of molecular techniques, plant breeders have improved rice production systems and minimized yield losses. In this article, we have summarized the current advanced molecular techniques used for controlling blast disease. With the advent of new technologies like marker-assisted selection, molecular mapping, map-based cloning, marker-assisted backcrossing and allele mining, breeders have identified more than 100 Pi loci and 350 QTL in rice genome responsible for blast disease. These Pi genes and QTLs can be introgressed into a blast-susceptible cultivar through marker-assisted backcross breeding. These molecular techniques provide timesaving, environment friendly and labour-cost-saving ways to control blast disease. The knowledge of host-plant interactions in the frame of blast disease will lead to develop resistant varieties in the future.

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“…Allele mining approaches have been intended to identify superior alleles of rice blast resistance genes such as Pita (Yang et al, 2007;Huang et al, 2008;Wang et al, 2008a;Ramkumar et al, 2010), Pikh (Ramkumar et al, 2010), Pi54 (Kumari et al, 2013), and Pi-2 (Hittalmani et al, 2013) from different cultivated rice varieties and wild species. The blast resistance genes Pi9, Pi2 and Piz-t tend to be alleles from different rice resources while physically on the same gene locus on rice chromosome 6, but their level of resistance spectra can be quite different Zhu et al, 2012). A similar situation was also seen in Pik, Pik-m, Pik-p, Pi1, Pi54 and Pi54rh (Ashikawa et al, 2008;Rai et al, 2011b;Yuan et al, 2011;Zhai et al, 2011;Das et al, 2012;Hua et al, 2012), which all reside on the same chromosome locus but are cloned independently from various varieties and show a differential level of resistance spectra to a collection of M. oryzae strains .…”

Section: Allele Mining and Blast Resistance Genesmentioning

confidence: 99%

Allele Mining Strategies: Principles and Utilisation for Blast Resistance Genes in Rice (Oryza sativaL.)

Ashkani

Yusop²,

Shabanimofrad³

et al. 2015

Current Issues in Molecular Biology

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Allele mining is a promising way to dissect naturally occurring allelic variants of candidate genes with essential agronomic qualities. With the identification, isolation and characterisation of blast resistance genes in rice, it is now possible to dissect the actual allelic variants of these genes within an array of rice cultivars via allele mining. Multiple alleles from the complex locus serve as a reservoir of variation to generate functional genes. The routine sequence exchange is one of the main mechanisms of R gene evolution and development. Allele mining for resistance genes can be an important method to identify additional resistance alleles and new haplotypes along with the development of allele-specific markers for use in marker-assisted selection. Allele mining can be visualised as a vital link between effective utilisation of genetic and genomic resources in genomics-driven modern plant breeding. This review studies the actual concepts and potential of mining approaches for the discovery of alleles and their utilisation for blast resistance genes in rice. The details provided here will be important to provide the rice breeder with a worthwhile introduction to allele mining and its methodology for breakthrough discovery of fresh alleles hidden in hereditary diversity, which is vital for crop improvement.

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The identification of Pi50(t), a new member of the rice blast resistance Pi2/Pi9 multigene family

Cited by 77 publications

References 39 publications

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Current advance methods for the identification of blast resistance genes in rice

Allele Mining Strategies: Principles and Utilisation for Blast Resistance Genes in Rice (Oryza sativaL.)

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