The identification of serum tuftsin by reverse-phase high-performance liquid chromatography and mass spectrometry

Naim, John O.; Desiderio, Dominic M.; Trimble, Joann; Hinshaw, J. Raymond

doi:10.1016/0003-2697(87)90389-7

Cited by 16 publications

(3 citation statements)

References 6 publications

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“…Similar results were obtained by raising the final concentration of TFA to 0.2%. Perfluorinated carboxylic acids with longer n-alkyl chains, such as pentafluoropropionic, heptafluorobutyric, perfluoropentanoic, perfluorohexanoic and perfluoroheptanoic acid, have been employed as alternatives to TFA for the separation of polar peptides and proteins 25,26 and for natural amino acids 27 and dipeptides. 28 Table 1).…”

Section: Optimization Of Lc and Ms Conditionsmentioning

confidence: 99%

Profiling histidine‐containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry

et al. 2004

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The histidine-containing dipeptides carnosine (CAR) and structurally related anserine (ANS) and homocarnosine (HCAR), widely distributed in vertebrate organisms, have recently been proposed as endogenous quenchers for highly cytotoxic a,b-unsaturated aldehydes generated by peroxidation. A sensitive, selective, specific and rapid liquid chromatographic/electrospray ionization tandem mass spectrometric assay was developed and validated for the simultaneous determination of these peptides in biological matrices in order to establish their plasma/tissue distribution. Samples (plasma or tissue homogenates from male rats) were prepared by protein precipitation with HClO The intra-and inter-day precisions were <10% (≤17.47% at the LOQ) and the intra-and inter-assay accuracies were within ±10% for all concentrations. The mapping profile in rat tissue gave the following results: the highest concentrations of CAR and ANS were found in skeletal muscles (soleus, gastrocnemius, tibialis), followed by the heart, cerebellum and brain (ANS below the LOQ). HCAR was found only in the brain and cerebellum. No histidine-containing dipeptides were detectable in plasma, liver, kidney and lung.

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Section: Optimization Of Lc and Ms Conditionsmentioning

confidence: 99%

Profiling histidine‐containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry

et al. 2004

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“…The observation indexes include: spleen immunology function (Tuftsin, IgM); liver function (ALT, ALB, TBL, AST); clinical outcome (blood loss during operation, operative mortality, incidence of rebleeding, blood routine, operative time, days in hospital) and dynamic scintigraphy of the transplanted splenic fragment engaging technetium 99m Tc-labeled heatdamaged erythrocytes ( 99m Tc-DRBC). Serum tuftsin was detected preoperatively and 2 months after operation by means of reverse-phase high-performance liquid chromatography, as described by Naim et al [5]. Serum IgM was measured (Beckman Array 360 chemistry Analyzer; Global Medical Instrumentation Inc, Clearwater, MN) preoperatively and 2 months after surgery.…”

Section: Design Of Studymentioning

confidence: 99%

Splenic autotransplantation and oesophageal transection anastomosis in patients with portal hypertension (26 years clinical observation)

et al. 2007

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The surgical treatment methods for cirrhosis patients complicated with portal hypertension are complicated. In this study, we evaluated the effectiveness of a new treatment strategy: splenic auto-transplantation and oesophageal transection anastomosis on 274 patients from three aspects: clinical observation, splenic immunology and portal dynamics. From 1979 to 2005, 274 cirrhosis patients with portal hypertension who underwent the new treatment strategy were followed up to observe different clinical indexes, which were then compared with those of the traditional surgery treatment. From 1999 to 2002, a randomized control trial (RCT) was performed on 40 patients to compare their immune function after operation. From 1994 to 2004, another RCT was carried out on 28 patients to compare the portal dynamics through three-dimensional dynamic contrast enhanced MR angiography (3D DEC MRA) investigation after operation. Among 274 patients (mean age 41.8 years), the emergency operative mortality (4.4%), selective operative mortality (2.2%), complication rate (17.9%), morbidity of hepatic encephalopathy (<1%), bleeding rate of portal hypertension gastritis (PHG) (9.1%), and morbidity of hepatic carcinoma (8%) were similar to those under traditional operation; the spleen immunology function (Tuftsin, IgM) decreased among the groups 2 months after operation. Through 3D DCE MRA, the cross section area, the velocity and volume of blood flow of main portal vein decrease significantly after operation in both groups, the auto transplantation group was significantly lower in velocity and volume of blood flow than in the control group. Splenic auto transplantation and esophageal transection anastomosis are a safe, effective, and reasonable treatment strategy for portal hypertension with varicial bleeding. It can not only correct hypersplenism but also completely stanch blood, and auto transplanted spleen in the retroperitoneal space can preserve immune function and establish abroad collateral circulation.

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“…Serum tuftsin was detected preoperatively and 2 months after operation by means of reverse-phase high-performance liquid chromatography, as described by Naim et al 10 Briefly, 5 mL of peripheral blood from each subject was collected in a nonsiliconized tube. Serum was isolated by centrifugation, and aliquots were frozen at -70°C for future assay.…”

Section: Measurement Of Serum Tuftsinmentioning

confidence: 99%

The Value of Partial Splenic Autotransplantation in Patients With Portal Hypertension

Zhang

Chen²,

Kaiser³

et al. 2002

Arch Surg

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Setting: University hospital. Patients: Twenty patients (19 men and 1 woman; aged 33-80 years) suffering from portal hypertension and liver cirrhosis were randomly allocated into 2 groups. Each group consisted of 10 patients. Interventions: All patients underwent modified Sugiura operation. In the control group, splenectomy was performed, while partial splenic autotransplantation into the retroperitoneal space was additionally completed in the splenic autotransplantation group. Main Outcome Measures: Serum tuftsin and IgM were measured preoperatively and 2 months after surgery. Dynamic scintigraphy with technetium Tc 99m-labeled heat-damaged erythrocytes was performed at 2-month intervals during the 8-month follow-up. Results: There was no statistical difference in the mortality of the groups. The preoperative levels of serum tuftsin and IgM showed no statistical difference between groups. However, although these measures had decreased remarkably in the control group 2 months after operation (PϽ.001 for serum tuftsin; P =.04 for serum IgM), they remained stable in the splenic autotransplantation group (P =.25 for serum tuftsin; P =.12 for serum IgM). Four patients within the splenic autotransplantation group showed positive scanning of the transplanted splenic fragment during follow-up, whereas there was no positive scanning in the control group. RESULTS MORBIDITY AND MORTALITY An 80-year-old patient from the splenic autotransplantation group, who had had severe chronic obstructive pulmonary disease for more than 30 years, died of

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The identification of serum tuftsin by reverse-phase high-performance liquid chromatography and mass spectrometry

Cited by 16 publications

References 6 publications

Profiling histidine‐containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry

Profiling histidine‐containing dipeptides in rat tissues by liquid chromatography/electrospray ionization tandem mass spectrometry

Splenic autotransplantation and oesophageal transection anastomosis in patients with portal hypertension (26 years clinical observation)

The Value of Partial Splenic Autotransplantation in Patients With Portal Hypertension

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