The identity of zona pellucida receptor on spermatozoa: An unresolved issue in developmental biology

Chiu, Philip C.N.; Lam, Kevin K.W.; Wong, Rachel C. W.; Yeung, William S.B.

doi:10.1016/j.semcdb.2014.04.016

Cited by 21 publications

(21 citation statements)

References 165 publications

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“…Adequate sperm motility was reported to be vital for successful fertilization (Olds-Clarke 1996). Our results were also similar to the analytical results in HolsteinFriesian bulls (Chiu et al 2014, Zhang et al 2015. The SNP g.-1256 A > C may regulate the expression of the bovine CAPN1 gene via transcription factor ZNF263 and cause significant potential phenotype diversity.…”

Section: Discussionsupporting

confidence: 86%

A g.-1256 A>C in the promoter region of CAPN1 is associated with semen quality traits in Chinese Holstein bulls

Cui¹,

Sun²,

Wang

et al. 2016

Reproduction

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The micromolar calcium-activated neutral protease gene (CAPN1) is a physiological candidate gene for sperm motility. However, the molecular mechanisms involved in regulating the expression of the CAPN1 gene in bulls remain unknown. In this study, we investigated the expression pattern of CAPN1 in testis, epididymis, and sperm at the RNA and protein levels by qRT-PCR, western blot, immunohistochemistry, and immunofluorescence assay. Results revealed that the expression of CAPN1 levels was higher in the sperm head compared with that in other tissues. Moreover, we identified a novel single-nucleotide polymorphism (g.-1256 A>C, ss 1917715340) in the noncanonical core promoter of the CAPN1 gene between base g.-1306 and g.-1012. Additionally, we observed greater sperm motility in bulls with the genotype CC than in those with the genotype AA (P<0.01), indicating that different genotypes were associated with the bovine semen trait. Furthermore, a higher fluorescence intensity of the C allele than that of the A allele at g. -1256 A>C was revealed by transient transfection in MLTC-1 cells and luciferase report assay. Finally, CAPN1 was highly expressed in the spermatozoa with the CC genotype compared with that with the AA genotype by qRT-PCR. This study is the first report on genetic variant g.-1256 A>C in the promoter region of CAPN1 gene association with the semen quality of Chinese Holstein bulls by influencing its expression. g.-1256 A>C can be a functional molecular marker in cattle breeding.

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Section: Discussionsupporting

confidence: 86%

A g.-1256 A>C in the promoter region of CAPN1 is associated with semen quality traits in Chinese Holstein bulls

Cui¹,

Sun²,

Wang

et al. 2016

Reproduction

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“…The interpretation of these observations has been controversial and whether the loss of fertility in the absence of the N-terminus of ZP2 was a direct or indirect effect on gamete recognition was not determined. Nor did these investigations experimentally address an essential role for glycans in sperm-zona interactions which long has been a central tenet of the molecular basis of gamete interactions (Abou-Haila et al, 2014; Chiu et al, 2014; Clark, 2014). …”

Section: Introductionmentioning

confidence: 99%

Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and ZP2 Cleavage to Prevent Polyspermy

Tokuhiro

Dean

2018

Developmental Cell

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The zona pellucida surrounding ovulated eggs regulates monospermic fertilization necessary for successful development. Using mouse transgenesis, we document that the N terminus of ZP2 is sufficient for sperm binding to the zona matrix and for in vivo fertility. Sperm binding is independent of ZP2 glycans and does not occur after complete cleavage of ZP2 by ovastacin, a zinc metalloendopeptidase stored in egg cortical granules. Immediately following fertilization, a rapid block to sperm penetration of the zona pellucida is established that precedes ZP2 cleavage but requires ovastacin enzymatic activity. This block to penetration is associated with release of zinc from cortical granules coincident with exocytosis. High levels of zinc affect forward motility of sperm to prevent their passage through the zona matrix. This transient, post-fertilization block to sperm penetration provides a temporal window to complete the cleavage of ZP2, which prevents sperm binding to ensure monospermy.

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“…Various physiological and nonphysiological agonists have been shown to activate mammalian AE including zona pellucida (ZP) and cumulus terminal monosaccharides [2–7]. Multiple sperm–carbohydrate binding events must occur simultaneously to stimulate AE and identification of the associated receptors is complex [8–11]. Thus, synthetic biomaterials to assess sperm AE induction reproducibly are required to clearly identify receptors responsible, to develop simple semen analysis assays, and to identify new targets for contraceptives [11].…”

Section: Introductionmentioning

confidence: 99%

“…Multiple sperm–carbohydrate binding events must occur simultaneously to stimulate AE and identification of the associated receptors is complex [8–11]. Thus, synthetic biomaterials to assess sperm AE induction reproducibly are required to clearly identify receptors responsible, to develop simple semen analysis assays, and to identify new targets for contraceptives [11]. Several groups have used neoglycoconjugates to explore the involvement of individual types of saccharides in AE [12–14].…”

Section: Introductionmentioning

confidence: 99%

Glycopolymer induction of mouse sperm acrosomal exocytosis shows highly cooperative self-antagonism

Rodolis

Huang

Sampson

2016

Biochemical and Biophysical Research Communications

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Identifying inducers of sperm acrosomal exocytosis (AE) to understand sperm functionality is important for both mechanistic and clinical studies in mammalian fertilization. Epifluorescence microscopy methods, while reproducible, are laborious and incompatible for high throughput screening. Flow cytometry methods are ideal for quantitative measurements on large numbers of samples, yet typically rely on the use of lectins that can interfere with physiologic AE-inducers. Here, we present an optimized triple stain flow cytometric method that is suitable for high-throughput screening of AE activation by glycopolymers. SYTO-17 and propidium iodide (PI) were used to differentiate cells based on their membrane integrity or viability, and membrane impermeable soybean trypsin inhibitor (SBTI) was used to monitor acrosome exocytosis. The SBTI/PI/SYTO-17 combination provides a positive screen for viability and AE of live sperm cells with minimal noise or false positives. A scattering gate enables the use of samples that may be contaminated with non-cellular aggregates, e.g., cryopreservation agents. This assay format enabled detailed analysis of glycopolymer dose response curves. We found that fucose polymer has a narrow effective dose range (EC50 = 1.6 µM; IC50 = 13.5 µM); whereas mannose polymer and β-N-acetylglucosamine polymer have broader effective dose ranges (EC50 = 1.2 µM and 3.4 µM, respectively). These results highlight the importance of testing inducers over a large concentration range in small increments for accurate comparison.

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The identity of zona pellucida receptor on spermatozoa: An unresolved issue in developmental biology

Cited by 21 publications

References 165 publications

A g.-1256 A>C in the promoter region of CAPN1 is associated with semen quality traits in Chinese Holstein bulls

A g.-1256 A>C in the promoter region of CAPN1 is associated with semen quality traits in Chinese Holstein bulls

Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and ZP2 Cleavage to Prevent Polyspermy

Glycopolymer induction of mouse sperm acrosomal exocytosis shows highly cooperative self-antagonism

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