The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node

Nygaard, Unni Cecilie; Ormstad, Heidi; Aase, Audun; Løvik, Martinus

doi:10.1016/j.tox.2004.07.018

Cited by 18 publications

(22 citation statements)

References 48 publications

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“…Single cell suspensions were prepared from each lymph node as previously described (Nygaard et al, 2004). After pooling of the two PLN cell suspensions from the same animal, the cell concentration was measured with a Coulter counter Z1 (Beckman Coulter Incorporated, FL, USA), and presented as the lymph node cell number (10 6 ) per PLN.…”

Section: Lymph Node Cell Preparationmentioning

confidence: 99%

“…PLN single cell suspensions were stained as previously described (Nygaard et al, 2004). In short, 5 × 10 6 cells/ml were stained with FITC-or PEconjugated monoclonal antibodies against CD19 (B lymphocyte marker), CD4 (T helper cell marker), CD86, CD23 (BD PharMingen, San Diego, CA, USA) or CD3 (T lymphocyte marker), MHC class II (Southern Biotech, Birmingham, AL, USA).…”

Section: Cell Phenotypingmentioning

confidence: 99%

“…We therefore wanted to study how the particles, alone or together with an allergen, affected the immune cells in the draining lymph node during the primary immune response. We have earlier shown that the IgE adjuvant effect of PSP (0.1 m diameter) was associated with increased primary cellular responses in the popliteal lymph node (PLN), peaking 5 days after subcutaneous injection into the footpad (Nygaard et al, 2004). In the present study we tested whether coarse and fine size fractions of ambient air particulate matter from different European cities alter the allergic immune response by modulating the expression of cell membrane molecules and the pattern of cytokine production in the draining lymph node cells.…”

Section: Introductionmentioning

confidence: 99%

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Ambient air particles from four European cities increase the primary cellular response to allergen in the draining lymph node

et al. 2005

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Section: Lymph Node Cell Preparationmentioning

confidence: 99%

Section: Cell Phenotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ambient air particles from four European cities increase the primary cellular response to allergen in the draining lymph node

et al. 2005

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“…IL-5 is also the key factor for the activation and/or proliferation of eosinophils. Several studies have reported that subcutaneous exposure to PS particles with allergen elevates the production of allergen-specific IgE and the expression of IL-4 by the draining lymph node cells ex vivo (20)(21). In addition, these results were associated with the proliferation/activation of B and T cells in the draining popliteal lymph nodes.…”

Section: Discussionmentioning

confidence: 95%

“…Recently, we have reported that intratracheal instillation of PS nanoparticles enhanced lung inflammation related to LPS, which is more prominent with the smaller nanoparticles than with the larger ones regarding overall trend (18). Nygaard et al have shown that the adjuvant effects on IgE response were initiated by subcutaneous injection of PS particles into the footpad (20)(21). In addition, the smaller PS particles (0.0588 and 0.202 urn) have exerted more intense adjuvant effects than the larger ones (1.053, 4.64, and 11.34 urn) (21).…”

Section: Discussionmentioning

confidence: 98%

Size Effects of Polystyrene Nanoparticles on Atopic Dermatitis-like Skin Lesions in NC/NGA Mice

Yanagisawa

Takano

Inoue

et al. 2010

Int J Immunopathol Pharmacol

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Nano-sized particles are diffusing in the environment with the development of nanotechnology. Polystyrene (PS) nanoparticles are modified industrial products and pharmaceutical agents, however, adverse effects of PS nanoparticles remain to be elucidated. In the present study, we investigated the effects of PS nanoparticles with different sizes on the atopic dermatitis (AD)-like skin lesions in NC/Nga mice assumed to show the skin barrier defect/dysfunction in the presence or absence of mite allergen. Male NC/Nga mice were injected intradermally with three different-sized PS nanoparticles (25, 50, or 100 nm) and/or mite allergen into their right ears. We evaluated clinical scores, ear thickening, histological findings and the local protein expression of inflammatory molecules in the ear and Ig production in serum. PS nanoparticles aggravated AD-like skin lesions related to mite allergen, which was paralleled by the local protein levels of interleukin-4, CCL2/monocyte chemotactic protein-I, CCL3/macrophage inflammatory protein-l alpha, and CCL4/macrophage inflammatory protein-l beta. In contrast, PS nanoparticles decreased interferon-y expression. Furthermore, exposure to PS nanoparticles induced ear swelling and CC-chemokine expression in the absence of allergen. These effects were greater with the smaller PS nanoparticles than with the larger ones regarding overall trend. These results suggest that exposure to PS nanoparticles under skin barrier defect/dysfunction can exacerbate AD-like skin lesions related to mite allergen in a size-dependent manner. The enhancing effects may be accounted for by T helper 2-biased immune responses. Furthermore, PS nanoparticles can evoke skin inflammation via the overexpression of CC-chemokines even in the absence of allergen in atopic subjects.Atopic dermatitis (AD) is a pruritic inflammatory skin disease and its prevalence has been increasing progressively in industrialized countries (1-2). The underlying immunological milieu of human atopic dermatitis (AD) is characterized by up-regulation of inflammatory cytokines, IgE overproduction in serum and the accumulation of inflammatory cells. In most cases, various factors including immunological abnormalities, skin barrier dysfunction, exposure to allergen such as mite, environmental toxicants and psychotic factors (3-4) can contribute to the initiation and/or progression of AD. In fact, many animal studies have shown that environmental toxicants including chemicals can be closely related to the increasing prevalence of allergic diseases including AD. Recently, we have reported that systemic exposure to di-(2-ethylhexyl) phthalate (DEHP), a typical environmental chemical as the most

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Nanomaterial—Live Cell Interface: Mechanism and Concern

Mukhopadhyay

2016

Advanced Materials Interfaces

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The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node

Cited by 18 publications

References 48 publications

Ambient air particles from four European cities increase the primary cellular response to allergen in the draining lymph node

Ambient air particles from four European cities increase the primary cellular response to allergen in the draining lymph node

Size Effects of Polystyrene Nanoparticles on Atopic Dermatitis-like Skin Lesions in NC/NGA Mice

Nanomaterial—Live Cell Interface: Mechanism and Concern

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