The Image Processing Handbook

Russ, John C.

doi:10.1201/9780203881095

Cited by 848 publications

(329 citation statements)

References 0 publications

Supporting

Mentioning

305

Contrasting

Unclassified

Order By: Relevance

“…EDM methods are a recent innovation in image processing (Butt and Maragos, 1998) and are applied to the image object and its skeleton for estimation of the thickness of long thin objects such as fibers (Russ, 1994). To our knowledge, this is the first report using EDM for quantification of vessel diameters in either lymphatics or blood vasculature.…”

Section: Discussionmentioning

confidence: 99%

“…The overall average of vessel diameter in lymphatics within a confocal image was estimated as follows, using Euclidean distance mapping applied to corresponding binary and skeletonized images, as first developed for measurement of diameter in thin fibers (Russ, 1994). A Euclidean distance map (EDM) was first generated by determining the distance d for every pixel in a lymph vessel object within the binary image to the closest background pixel using the program edm.c (available from P. Parsons and M. Vickerman).…”

Section: Quantification Of Lymphatic Vesselsmentioning

confidence: 99%

See 1 more Smart Citation

Lymphangiogenesis by blind‐ended vessel sprouting is concurrent with hemangiogenesis by vascular splitting

et al. 2006

View full text Add to dashboard Cite

Development of effective vascular therapies requires the understanding of all modes of vessel formation involved in angiogenesis (here termed "hemangiogenesis") and lymphangiogenesis. Two major modes of vessel morphogenesis include sprouting of a new vessel from a preexisting vessel and splitting of a preexisting parent vessel into two offspring vessels. In the quail chorioallantoic membrane (CAM) during mid-development (embryonic days E6 -E9), lymphangiogenesis progressed primarily via blind-ended vessel sprouting. Isolated lymphatic endothelial progenitor cells were recruited to the tips of growing vessels. During concurrent hemangiogenesis, parent blood vessels expanded from the capillary network and split into offspring vessels, accompanied by transient capillary expression of alpha smooth muscle actin (␣SMA) and recruitment of polarized mural progenitor cells. Lymphatics and blood vessels were identified by confocal/fluorescence microscopy of vascular endothelial growth factor (VEGF) receptor VEGFR-2, ␣SMA (specific to CAM blood vessels), homeobox transcription factor Prox1 (specific to lymphatics), and the quail hematopoetic marker, QH-1. VEGFR-2 was expressed intensely in isolated cells and lymphatics, and moderately in blood vessels. Prox1 was absent from isolated progenitor cells prior to lymphatic recruitment. Exogenous vascular endothelial growth factor-165 (VEGF 165 ) increased blood vessel density and anastomotic frequency without changing endogenous modes of vascular/lymphatic vessel formation or marker expression. Although VEGF 165 is a key cellular regulator of hemangiogenesis and vasculogenesis, the role of VEGF 165 in lymphangiogenesis is less clear. Interestingly, VEGF 165 increased lymphatic vessel diameter and density as measured by novel Euclidean distance mapping, and the antimaturational dissociation of lymphatics from blood vessels, accompanied by lymphatic reassociation into homogeneous networks. Published 2006 Wiley-Liss, Inc. †

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Quantification Of Lymphatic Vesselsmentioning

confidence: 99%

Lymphangiogenesis by blind‐ended vessel sprouting is concurrent with hemangiogenesis by vascular splitting

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Subsequent analysis of these microscopic images using digital image correlation methods (see the Supporting Material) allowed for determination of the time-varying displacement field of the worm's internal structures as they deform under applied stress (middle panels of Fig. 6 A) (48). The strain can then be calculated using the Cauchy transformation between displacement and strain in a body undergoing pure deformation (49),…”

Section: Structural Deformation Of Internal Organsmentioning

confidence: 99%

Worms under Pressure: Bulk Mechanical Properties of C. elegans Are Independent of the Cuticle

Gilpin

Uppaluri

Brangwynne

2015

Biophysical Journal

View full text Add to dashboard Cite

The mechanical properties of cells and tissues play a well-known role in physiology and disease. The model organism Caenorhabditis elegans exhibits mechanical properties that are still poorly understood, but are thought to be dominated by its collagen-rich outer cuticle. To our knowledge, we use a novel microfluidic technique to reveal that the worm responds linearly to low applied hydrostatic stress, exhibiting a volumetric compression with a bulk modulus, k ¼ 140 5 20 kPa; applying negative pressures leads to volumetric expansion of the worm, with a similar bulk modulus. Surprisingly, however, we find that a variety of collagen mutants and pharmacological perturbations targeting the cuticle do not impact the bulk modulus. Moreover, the worm exhibits dramatic stiffening at higher stresses-behavior that is also independent of the cuticle. The stress-strain curves for all conditions can be scaled onto a master equation, suggesting that C. elegans exhibits a universal elastic response dominated by the mechanics of pressurized internal organs.

show abstract

“…For example, if an experiment requires the quantitation of only the cytoplasmic component of a fluorescent probe, the ImageStream system can be directed to use a Boolean combination of the brightfield and nuclear segmentation masks to define a ring-like search area encompassing only cytoplasm (21). Using biological context and existing information from other channels to narrowly define the search space has the benefit of substantially reducing the number of pixels in the search area for a given object.…”

Section: Effects Of Pixel Count and Multimode Imagery On Detection Limitmentioning

confidence: 99%

Sensitivity measurement and compensation in spectral imaging

Ortyn¹,

Hall²,

George³

et al. 2006

Cytometry Pt A

108

View full text Add to dashboard Cite

Background: The ImageStream system combines advances in CCD technologies with a novel optical architecture for high sensitivity and multispectral imaging of cells in flow. The sensitivity and dynamic range as well as a methodology for spectral compensation of imagery is presented. Methods: Multicolored fluorescent beads were run on the ImageStream and a flow cytometer. Four single color fluorescent control samples of cells were run to quantify spectral overlap. An additional sample, labeled with all colors was run and compensated in six spectral channels. Results: Analysis of empirical data for sensitivity and dynamic range matched theoretical predictions. The ImageStream system demonstrated fluorescence sensitivity comparable to a PMT-based flow cytometer. A methodology for addressing spectral overlap, individual pixel ano-

show abstract

The Image Processing Handbook

Cited by 848 publications

References 0 publications

Lymphangiogenesis by blind‐ended vessel sprouting is concurrent with hemangiogenesis by vascular splitting

Lymphangiogenesis by blind‐ended vessel sprouting is concurrent with hemangiogenesis by vascular splitting

Worms under Pressure: Bulk Mechanical Properties of C. elegans Are Independent of the Cuticle

Sensitivity measurement and compensation in spectral imaging

Contact Info

Product

Resources

About