The immortalized cell lines with differentiation potentials: Their establishment and possible application

GT1-7 Cell-Based cytoxicity screening assay on 96-well microplates as a platform for the safety assessment of genetically modifiedGerbera hybridaextracts

“…Immortalized cells may respond differently than other in vitro systems (Harry and Tiffany-Castiglioni, 2005), but our results with pure compounds proved that similar cytotoxicity results are obtained in GT1-7 cells if compared to other cell lines. In fact, the use of immortalized cell lines within screening platforms can be a strategy to pursue, as recently suggested by Obinata (2007). For measuring cell viability, the fluorescence signal produced by dead or dying cells selectively stained by propidium iodide was used here and shown to be reliable, with the particular advantage of only requiring two pipetting steps (for propidium iodide and digitonine), making it highly amenable to automation.…”

Cellular models for disease exploring and drug screening

“…Given that primary cells cannot stably proliferate, a series of immortalized cell models are constructed by ectopic oncogene or telomerase gene transduction, or a combination of both (Obinata, 2007). The immortalized cells derived from human or animals may solve the resource limitation of primary cells (Darimont, 2003;Obinata, 2007;Pang et al, 2008), what is more, the immortalization test is currently used as a risky assessment procedure of drug candidates (Landolph, J.R., 2006;Pang et al, 2008).…”

“…The immortalized cells derived from human or animals may solve the resource limitation of primary cells (Darimont, 2003;Obinata, 2007;Pang et al, 2008), what is more, the immortalization test is currently used as a risky assessment procedure of drug candidates (Landolph, J.R., 2006;Pang et al, 2008).…”

Development of Oral Epithelial Cell Line ROE2 with Differentiation Potential from Transgenic Rats Harboring Temperature-Sensitive Simian Virus40 Large T-Antigen Gene

“…Previous reports demonstrated that the SV40 large T-antigen can stabilize cell-type-specific functions in oral epithelial immortalized cell lines [9, 20]. Moreover, transgenic (TG) mice harboring tsSV40 large T-antigen have been very useful for establishing immortalized cell lines from many kinds of tissues including epithelia [27, 28]. Using TG mice, several kinds of epithelial cell lines with specific functions have been developed from kidney distal tubule [45, 54], gastric fundic mucosa [39], gingival epithelium [12], tracheal epithelium [40], intestinal epithelium [42, 52], and epididymal epithelium [3].…”