The immune-enhancing activity of Cervus nippon mantchuricus extract (NGE) in RAW264.7 macrophage cells and immunosuppressed mice

Hong, Se Hyang; Ku, Jin Mo; Kim, Hyo In; Ahn, Chang Hyuk; Park, Soo-Hyun; Seo, Hye Sook; Shin, Yong Cheol; Ko, Seong‐Gyu

doi:10.1016/j.foodres.2017.06.053

Cited by 25 publications

(21 citation statements)

References 40 publications

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“…Many studies reported that increased NO via enhanced iNOS expression activates macrophages and improves immune-enhancing activity (Ha et al, 2013; Hong et al., 2017; Li et al, 2017). Li et al (2017) and Hong et al (2017) reported that extracted materials from Radix astragali and Cervus nippon mantchuricus have immune-enhancing activity. These extracted materials increased the iNOS mRNA expression and NO production in RAW 264.7 macrophages without causing cytotoxicity.…”

Section: Resultsmentioning

confidence: 99%

“…The modulation of immune response is especially important for people who have impaired immune responses such as the elderly and cancer patients, because it plays a crucial role in diseases prevention (Salvioli et al, 2006). Accordingly, immunomodulation induced by active substances have been the focus of many studies (Castro et al, 2008; Hong et al, 2017; Shen et al, 2017). They found new active substances from natural materials which are herb, egg, and grape etc.…”

Section: Introductionmentioning

confidence: 99%

“…Macrophage activation is associated with the major signaling pathways such as mitogen activated protein kinase (MAPK) which include p38 MAPK, extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK) (Han et al, 2009). Therefore, many researchers have been studying new substances with immunomodulatory and immunostimulatory activities that activate macrophages via MAPK pathway (Hong et al, 2017; Li et al, 2017; Seo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages

Lee¹,

Ahn²,

Paik³

2018

Korean J Food Sci Anim Resour

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Ovotransferrin (OTF) is a well-known protein of the transferrin family with strong iron chelating activity, resulting in its antimicrobial activity. Furthermore, OTF is known to have antioxidant, anticancer, and antihypertensive activities. However, there have been few studies about the immune-enhancing activity of OTF. In current study, we investigated the immune-enhancing activity of OTF using the murine macrophage cells in vitro. The effect of OTF on production of pro-inflammatory mediators and cytokines were determined using Griess assay and quantitative real-time PCR. Using Neutral Red uptake assay, we confirmed the effect of OTF on phagocytic activity of macrophages. Ovotransferrin significantly increased the production of nitric oxide (NO) and secretion of inducible nitric oxide synthase (iNOS) mRNA with no cytotoxic activity. Ovotransferrin (2 mg/mL) stimulated NO production up to 31.9±3.5 μM. Ovotransferrin significantly increased the mRNA expression levels of pro-inflammatory cytokines which are tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), and IL-6: OTF (2 mg/mL) treatment increased the secretion of mRNA for TNF-α, IL-1β, and IL-6 by 22.20-, 37.91-, and 6.17-fold of the negative control, respectively. The phagocytic activity of macrophages was also increased by OTF treatment significantly compared with negative control. Also, OTF treatment increased phosphorylation level of MAPK signaling pathways. These results indicated that OTF has immune-enhancing activity by activating RAW 264.7 macrophages via MAPK pathways.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages

Lee¹,

Ahn²,

Paik³

2018

Korean J Food Sci Anim Resour

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“…In our previous study, it was found that NGE alleviated neutropenia and activated macrophages in vivo and in vitro (18). In previous studies, it has also been found that the oral administration of NGE is useful for the treatment of memory loss (19) and bone resorption (20), as an anti-aging treatment (21), for the activation of macrophages (22), enhancement of immune system activity (23), and the control of various inflammatory diseases (24)(25)(26). It has been confirmed that NGE is effective against a variety of inflammatory diseases, therefore, it is expected to be effective for inflammatory skin diseases, including AD.…”

Section: Oral Administration Of Cervus Nippon Mantchuricus Extract Sumentioning

confidence: 95%

Oral administration of Cervus nippon mantchuricus extract suppresses 2,4-dinitrochlorobenzene-induced atopic dermatitis in BALB/c mice and inflammatory effects in mast cells

Hong

Kim

et al. 2018

Int J Mol Med

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Cervus nippon mantchuricus extract, known as nok‑gol (NGE) in Korean, is useful for the treatment of various inflammatory diseases, including bone resorption and neutropenia. However, NGE has not been widely investigated, and its efficacy and safety remain to be fully elucidated. In the present study, histological analysis, blood analysis, reverse transcription‑semi-quantitative polymerase chain reaction analysis and enzyme‑linked immunosorbent assays were performed to verify the inhibitory effect of NGE on atopic dermatitis (AD) in BALB/c mice and on inflammatory effects in HMC‑1 human mast cells. NGE suppressed the development of AD in mice, and decreased the infiltration of inflammatory cells, mast cells and CD4+ T cells into AD skin lesions. NGE also decreased leukocyte levels induced by 2,4‑dinitrochlorobenzene (DNCB). NGE alleviated AD‑like inflammatory symptoms in mice by suppressing the production of CD4+ T cells. NGE downregulated the mRNA expression of inflammatory cytokines induced by DNCB. It also decreased the serum immunoglobulin E concentration and inflammatory cytokine levels in DNCB‑treated BALB/c mice. The in vitro experiments demonstrated that NGE reduced the phorbol 12‑myristate 13‑acetate + ionomycin‑induced expression of pro‑inflammatory cytokines interleukin (IL)‑4, IL‑13, tumor necrosis factor‑α, and IL‑6 in HMC‑1 cells. Taken together, the results of the present study indicated that NGE suppressed the progression of DNCB‑induced AD in BALB/c mice and reduced inflammatory effects in HMC‑1 cells. This suggests that NGE may be a useful drug for the treatment of AD.

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“…More recently, immunomodulators from natural extracts have attracted much attention in the field due to their lesser side-effects when used in humans [8,9]. For example, Hong et al [10] showed that Cervus nippon mantchuricus extract (NGE) has immuno-enhancing effects on RAW264.7 macrophage cells in immunosuppressed mice. Purified leaf extracts of Melia azedarach L. (CDM) exerted anti-herpetic activity, inhibited NFκB translocation to the nucleus, and modulated both interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) responses in macrophages in one recent study [11].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Effects of the Meretrix Meretrix Oligopeptide (QLNWD) on Immune-Deficient Mice

Zhang

Wang³

et al. 2019

Molecules

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The aim of this study was to explore the immunomodulatory effects of the Meretrix meretrix oligopeptide (MMO, QLNWD) in cyclophosphamide (CTX)-induced immune-deficient mice. Compared to untreated, CTX-induced immune-deficient mice, the spleen and thymus indexes of mice given moderate (100 mg/kg) and high (200 mg/kg) doses of MMO were significantly higher (p < 0.05), and body weight loss was alleviated. Hematoxylin-eosin (H&E) staining revealed that MMO reduced spleen injury, thymus injury, and liver injury induced by CTX in mice. Furthermore, MMO boosted the production of immunoglobulin G (IgG) and hemolysin in the serum and promoted the proliferation and differentiation of spleen T-lymphocytes. Taken together, our findings suggest that MMO plays a vital role in protection against immunosuppression in CTX-induced immune-deficient mice and could be a potential immunomodulatory candidate for use in functional foods or immunologic adjuvants.

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The immune-enhancing activity of Cervus nippon mantchuricus extract (NGE) in RAW264.7 macrophage cells and immunosuppressed mice

Cited by 25 publications

References 40 publications

In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages

In Vitro Immune-Enhancing Activity of Ovotransferrin from Egg White via MAPK Signaling Pathways in RAW 264.7 Macrophages

Oral administration of Cervus nippon mantchuricus extract suppresses 2,4-dinitrochlorobenzene-induced atopic dermatitis in BALB/c mice and inflammatory effects in mast cells

Immunomodulatory Effects of the Meretrix Meretrix Oligopeptide (QLNWD) on Immune-Deficient Mice

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