The Immunization of Protoscolices P29 DNA Vaccine on Experimental Cystic Echinococosis in Balb/c Mice

Gharibi, Zahra; Rahdar, Mahmoud; Pirestani, Majid; Tavalla, Mehdi; Tabandeh, Mohammad Reza

doi:10.1007/s11686-021-00367-7

Cited by 6 publications

(5 citation statements)

References 28 publications

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“…Our previous data showed the immune protection of rEg.P29 against E . granulosus -infected mice and sheep was 94.3% and 98.6% [ 14 , 15 ], respectively. However, the protection and mechanism of rEg.P29 against E .…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study found the protective efficacy of rEg.P29 in sheep and mouse models with secondary infection of E . granulosus were 94.5% and 96.6%, respectively [ 14 , 15 ]. In addition, rEg.P29 can induce mice to produce high levels of IgG, IgM, IgE and IgA antibodies, and significantly increase IFN-γ secretion from CD4 + T and CD8 + T cells [16] .…”

Section: Introductionmentioning

confidence: 99%

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Immunoprotective effect and mechanism of rEg.P29 against CD4<sup>+</sup> T cell-deficient mice with <italic>Echinococcus multilocularis</italic> infection

Li,

Zhu,

et al. 2023

ABBS

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Alveolar echinococcosis (AE) is a zoonotic parasitic disease caused by infection with the larval stage of Echinococcus multilocularis and a major challenge to human public health. Vaccines are the most effective way to prevent and control infectious diseases. We previously revealed that the Echinocuccus granulosus recombinant protein P29 is a good vaccine candidate against E . granulosus . However, the protective and immunological mechanism of rEg.P29 against E . multilocularis remain unclear. In this study, CD4 + T cell-deficient mice are transferred with spleen CD4 + T cells isolated from wild-type mice and subjected to rEg.P29 immunization, and then these immunized mice are infected with E . multilocularis . The cyst inhibition rate is calculated by weighing the body and cyst weights. The level of antibody is detected by ELISA. Flow cytometry is used to detect the level of IFN-γ production by CD4 + T and CD8 + T cells. The cytokines in culture supernatant are detected by ELISA. The expressions of CD44 and CD62L on memory T cells are determined by flow cytometry. The results show the cyst inhibition rate is 41.52% after adoptive transfer of CD4 + T cells. Furthermore, the levels of IgG, IgM, IgA and IgE in serum are significantly increased compared with those in the PBS group. The IFN-γ-secretion by CD8 + T cells and the level of IFN-γ in culture supernatant are obviously increased; and the number of CD4 + T cells is increased, but the number of IFN-γ producing CD4 + T cells has no significant difference compared with PBS group. In addition, the number of CD44 + CD62L ‒ CD8 + memory T cells in the spleen is significantly increased, while the number of CD44 ‒ CD62L + CD8 + memory T cells is not significantly altered. Collectively, rEg.P29 can alleviate E . multilocularis infection by inducing humoral immune responses and CD8 + T cell responses.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunoprotective effect and mechanism of rEg.P29 against CD4<sup>+</sup> T cell-deficient mice with <italic>Echinococcus multilocularis</italic> infection

Li,

Zhu,

et al. 2023

ABBS

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“…The mannose-modified lipid calcium phosphate nanoparticle vaccine changes the microenvironment of the tumor, which boosts the immune response against it [185]. One way it does this is by activating T cells, T cells are an important part of the immune system and play a key role in recognizing and eliminating tumor cells [186][187][188].…”

Section: Activation Of T Cells By Mannose Modified Lipid Calcium Phos...mentioning

confidence: 99%

A Mannose-Modified Lipid Calcium Phosphate Nanoparticle Vaccine Increased the Anti-Tumor Immune Response by Modulating the Tumor Microenvironment

Wu,

Qian,

et al. 2024

Preprint

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With the rapid development of tumor immunotherapy, nanoparticle vaccines have attracted much attention as a potential therapeutic strategy. The potential role of the mannose-modified lipid calcium phosphate nanoparticle vaccine in enhancing the anti-tumor immune response was investigated. The aim of this study was to investigate the effect of mannose modification on the immune response of nanoparticles in regulating the tumor microenvironment through systematic review and analysis and to explore its potential clinical application in tumor therapy. Currently, despite the potential advantages of nanoparticle vaccines in immunotherapy, achieving an effective immune response in the tumor microenvironment remains a challenge. Tumor immune escape and overexpression of immunosuppressive factors limit its clinical application. Therefore, this study will explore how to intervene in the immunosuppressive mechanism in the tumor microenvironment through mannose-modified lipid calcium phosphate nanoparticle vaccines so as to improve the immunotherapy effect of tumor patients and provide new ideas and strategies for the field of tumor therapy.

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“…For example, in people undergoing chemotherapy, the incidence of this infection is high, which can indicate the role of the immune system in the occurrence of this infection (1,15,16). Vaccines are the best tools for preventing parasitic diseases (17). Trichomoniasis is often asymptomatic and therefore untreated, creating reservoirs of T. vaginalis that allow the disease to spread in the community.…”

Section: Incidence Of Trichomonas Vaginalis Infection Among Female Sm...mentioning

confidence: 99%

Incidence of Trichomonas vaginalis Infection Among Female Smokers: A Narrative Review

et al. 2022

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Background: The harmful effects of smoking on women’s bodies have been proven. One of these effects is an increase in sexually transmitted diseases, including Trichomonas vaginalis infection. Smoking is one of the main risk factors for infections in the respiratory, digestive, and reproductive systems in humans. Mechanisms of susceptibility to infection in smokers may involve changes in the host’s structural, functional, and immunological defenses. T. vaginalis infection is one of the non-viral sexually transmitted diseases and the most common curable sexually transmitted infection in the world, which can increase the risk of pelvic inflammatory disease and HIV transmission. This infection is still relatively common, especially in women who smoke and in groups with lower socioeconomic status. We aimed to present a compressive review of the probability of being infected with T. vaginalis in smokers compared to non-smokers. Materials and Methods: PubMed and Google databases were searched for research articles related to smoking and its association with T. vaginalis infection. Results: The smoking status of the person is an independent risk factor for T. vaginalis infection and leads to an increased likelihood of developing this infection. Conclusion: Finally, the results of various studies showed that the prevalence of T. vaginalis infection is higher in smokers than in non-smokers.

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The Immunization of Protoscolices P29 DNA Vaccine on Experimental Cystic Echinococosis in Balb/c Mice

Cited by 6 publications

References 28 publications

Immunoprotective effect and mechanism of rEg.P29 against CD4<sup>+</sup> T cell-deficient mice with <italic>Echinococcus multilocularis</italic> infection

Immunoprotective effect and mechanism of rEg.P29 against CD4<sup>+</sup> T cell-deficient mice with <italic>Echinococcus multilocularis</italic> infection

A Mannose-Modified Lipid Calcium Phosphate Nanoparticle Vaccine Increased the Anti-Tumor Immune Response by Modulating the Tumor Microenvironment

Incidence of Trichomonas vaginalis Infection Among Female Smokers: A Narrative Review

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The Immunization of Protoscolices P29 DNA Vaccine on Experimental Cystic Echinococosis in Balb/c Mice

Cited by 6 publications

References 28 publications

Immunoprotective effect and mechanism of rEg.P29 against CD4&lt;sup&gt;+&lt;/sup&gt; T cell-deficient mice with &lt;italic&gt;Echinococcus multilocularis&lt;/italic&gt; infection

Immunoprotective effect and mechanism of rEg.P29 against CD4&lt;sup&gt;+&lt;/sup&gt; T cell-deficient mice with &lt;italic&gt;Echinococcus multilocularis&lt;/italic&gt; infection

A Mannose-Modified Lipid Calcium Phosphate Nanoparticle Vaccine Increased the Anti-Tumor Immune Response by Modulating the Tumor Microenvironment

Incidence of Trichomonas vaginalis Infection Among Female Smokers: A Narrative Review

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Immunoprotective effect and mechanism of rEg.P29 against CD4<sup>+</sup> T cell-deficient mice with <italic>Echinococcus multilocularis</italic> infection

Immunoprotective effect and mechanism of rEg.P29 against CD4<sup>+</sup> T cell-deficient mice with <italic>Echinococcus multilocularis</italic> infection