The immunohistochemical distribution of collagens type IV, V, VI and of laminin in the human oral mucosa

Becker, Jürgen C.; Schuppan, Detlef; Hahn, Eckhart G.; Albert, Gabriella; Reichart, Peter A.

doi:10.1016/0003-9969(86)90125-1

Cited by 55 publications

(22 citation statements)

References 30 publications

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“…Localization of CK 1/2/10/11, CK 5/6, CK 13, CK 14, CK 17 and CK 19 was similar in both tissue preparations and comparable to the results of previous studies [Shabana et al, 1989;Ouhayoun et al, 1990]. Staining patterns of collagen IV and laminin, components of the subepithelial and vascular basal membrane [Becker et al, 1986], were similar in frozen sections and in sections of MMA-embedded material.…”

Section: Discussionsupporting

confidence: 75%

Immunohistochemical Comparison of Markers for Wound Healing on Plastic-Embedded and Frozen Mucosal Tissue

Mai

Gedrange²,

Leonhardt³

et al. 2008

Cells Tissues Organs

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Immunohistologic investigations of wound healing in human oral mucosa require specific cell biological markers as well as consecutive small biopsies. Small specimens are ideally embedded in plastic (methylmethacrylate, MMA) resin due to their miniature size. This limits the use of antibodies for these markers. In this immunohistochemical study, the distribution of wound healing markers, e.g. cytokeratin (CK), laminin, collagen IV, vimentin, vinculin and fibronectin, were compared between semithin sections of plastic-embedded tissue and frozen sections of mucosal tissue in order to assess their use for future investigations. The antibodies against laminin, collagen IV and CK 1/2/10/11, 5/6, 13, 14, 17, 19 gave comparable staining patterns on cryostat sections of attached mucosa and on semithin sections of MMA-embedded attached mucosa. In the epithelial cell layers, the following distribution of CK immunostaining was observed: The basal cell layer was positive for CK 5/6, CK 14 and CK 19; the intermediate cell layer for CK 13, CK 17 and CK 1/2/10/11, and the superficial cell layer for CK 13 and CK 1/2/10/11. For most of these antibodies, enzyme digestion with 0.1% trypsin was adequate for demasking the antigens, except for anti-CK 14, anti-CK 17 and anti-laminin; predigestion with 0.4% pepsin in 0.01 N HCl gave similar staining results. The antibodies against vimentin, vinculin, fibronectin and CK 4 showed no affinity or a reciprocal reaction on the semithin sections. Therefore, the antibodies against CK 1/2/10/11; 5/6; 13; 14; 17, and 19, as well as the basement proteins laminin and collagen IV are deemed markers suitable on semithin sections of plastic-embedded attached oral mucosa.

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Section: Discussionsupporting

confidence: 75%

Immunohistochemical Comparison of Markers for Wound Healing on Plastic-Embedded and Frozen Mucosal Tissue

Mai

Gedrange²,

Leonhardt³

et al. 2008

Cells Tissues Organs

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“…Both collagen types are basically important to maintain regular structural functions [2,8,12]. Furthermore, it has been discussed that collagen type VI is involved in cell-cell interaction [1].…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical localization of collagen types I and VI in human skin wounds

Betz

Nerlich²,

Wilske

et al. 1993

Int J Leg Med

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Summary.A total of 74 human skin wounds were investigated and collagen types I and VI were localized in the wound area by immunohistochemistry. Collagen type I appeared in the form of ramifying string-like structures after approximately 5-6 days, but positive reactions in the form of a spot-like staining around isolated fibroblasts also occurred in a skin wound aged 4 days. Collagen VI was detectable after a post-infliction interval of at least 3 days showing a strongly positive reacting network associated with fibroblasts in the wound area. Both collagens appeared almost constantly after a wound age of 6-7 days and could also be found in wounds aged a few months. Therefore, although a positive reaction for collagen type I in the form of string-like and ramifying structures around wound fibroblasts indicates a wound age of at least 5-6 days, a spot-like positive staining for collagen type I cannot exclude a wound age of at least 4 days. A positive staining for collagen type VI represents a postinfliction time of 3 days or more. The almost constant appearance of these collagen types suggests that negative results in a sufficient number of specimens indicate a wound age of less than 6-7 days, but cannot completely exclude longer post-infliction intervals. Since collagen type I and VI are also found in the granulation/scar tissue of lesions with advanced wound age, the immunohistochemical analysis of these proteins provides no further information for an age determination of older skin wounds.

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“…In general, matrix components include collagen type III which contributes to bone formation (Keene et al, 1991) and pre-collagen type III is found in remodeled bone (Becker et al, 1986). Aigner et aL, (1993) reported that in the superficial and upper middle layers of the osteoarthitic cartilage, chondrocyte clusters and single chondrocytes were identified expressing collagen type III as well as type II, but not collagen type I.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Study of Extracellular Matrices and Elastic Fibers in a Human Sternoclavicular Joint

Shimada

Takeshige

Moriyama

et al. 1997

Okajimas Folia Anatomica Japonica

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Summary: In this study, we clarified the distribution of elastic and oxytalan fibers in a human sternoclavicular joint (SCJ) using a color image system and in extracellular matrices using immunoperoxidase staining. Fine elastic fibers (EFs) were scattered in the fibrous layer of the sternoclavicular disk. This articular disk was composed of a collagenous bundle on the sternum side of the articular disk in the SCJ and cellular components including connective tissue on the clavicular side of the articular disk. The thickness of the disk gradually increased from the inferior to superior portion. Collagen fibers type I, III and V and other extracellular matrices (ECMs) were detected in the hypertrophic zone in the clavicular and sternum side of the SCJ and in the connective tissue of the articulatio condylar. On the cervical surface of the articular disk, cellular activity was higher than on the sternum surface.The human sternoclavicular joint (SCJ) resembles the temporomandibular joint (TMJ) from the viewpoint of having a cartilaginous head and an articular disk, but details of the morphology and cellular activity of these joints on the immunohistochemical level are unknown. Moreover, some investigators have reported reconstructing the TMJ using sternoclavicular and costochondral grafts (Dingman and Grabb, 1964;Wolford et aL, 1994;MacIntosh and Henny, 1977). The success rate of reconstruction is higher than that of use of synthetic implants (Wolford et al. , 1994). Ellis and Carlson (1986) also indicated similarities between the SCJ and TMJ in the monkey. However, the cellular activity, development and functional property of the SCJ have not yet been determined. In particular, the ECMs are functionally and developmentally associated with cell activity (Takahashi et aL, 1995;Mizoguchi et al., 1997). Therefore, we clarified the distributions of the EF and ECM components in the human SCJ and compared them with those in the human TMJ. Material and Methods Preparation of tissueAdult humans with non-gross pathological changes were selected at autopsy from donors (twenty-four males aged 27 to 70 years) for a histochemical analysis at microscopic levels. The sternoclavicular joints were quickly removed and immediately fixed in a 3.7% solution of formaldehyde for one day at 4 °C. After washing them in running water, they were dehydrated in a graded series of ethanol (50, 70, 90 and 95% and absolute) followed by paraffin embedding. Paraffin blocks were cut into 3-gm-thick frontal serial sections. Staining and analysis of elastic and oxytalan fibersSections were stained by the following methods: (1) Elastica-van Gieson staining was performed to demonstrate the locations of elastic fibers.(2) Elastica-van Gieson staining after incubation in a preoxidizing solution (pH 1.5) containing 0 .3% ICMn04 and 0.3% H2SO4 was performed to visualize oxytalan fibers (modified version of the method

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The immunohistochemical distribution of collagens type IV, V, VI and of laminin in the human oral mucosa

Cited by 55 publications

References 30 publications

Immunohistochemical Comparison of Markers for Wound Healing on Plastic-Embedded and Frozen Mucosal Tissue

Immunohistochemical Comparison of Markers for Wound Healing on Plastic-Embedded and Frozen Mucosal Tissue

Immunohistochemical localization of collagen types I and VI in human skin wounds

Immunohistochemical Study of Extracellular Matrices and Elastic Fibers in a Human Sternoclavicular Joint

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