The Immunomodulatory and Regenerative Effect of Biodentine™ on Human THP‐1 Cells and Dental Pulp Stem Cells: In Vitro Study

Abstract

Background. Pulp tissue affected by deep caries and trauma can be protected by vital pulp therapies in which pulp regeneration success depends on the degree of pulp inflammation and the presence of regenerative signals. Reparative dentinogenesis requires dental pulp stem cell (DPSC) activity which can be stimulated by many bioactive molecules to repair the dentine, mediating a balance between the inflammatory response and the reparative events. Therefore, this study was performed in order to investigate the immune-inflammatory effect of Biodentine capping material on DPSCs and macrophages. Method. THP-1, a human monocytic cell line, was differentiated to macrophages, and flow cytometry was used to analyze the expressions of specific macrophage markers. LPS-mediated infection was created for macrophages and DPSCs followed by treatment with Biodentine. CBA array was used to investigate the cytokine secretion followed by qPCR. Migration potential of treated DPSCs was also determined. Results. Our results showed that THP-1 cell line was successfully differentiated into macrophages as shown by surface marker expression. CBA array and qPCR results showed that Biodentine-treated DPSCs and macrophages upregulated anti-inflammatory cytokines and downregulated proinflammatory cytokines. Also, Biodentine enhances the migration potential of treated DPSCs. Conclusion. Biodentine capping material mediated the polarization of M1 to M2 macrophages suggestive of tissue repair properties of macrophages and enhanced the anti-inflammatory cytokines of DPSCs responsible for dentine-pulp regeneration.

Role of biodentine in endodontics: a bibliometric and scientometric analysis

“…Finally, the keywords related to biodentine in endodontics point towards a multidisciplinary strategy. [48][49][50][51][52][53] Thus, the co-occurrence network of terms reveals the salient content of articles and research topics as a whole in the form of clusters 54,55 . In this way, co-occurrence maps are illuminating because they show how different fields of study contribute to the genesis of scientific articles [56][57][58][59] .…”

Effects of Staphylococcus aureus on stem cells and potential targeted treatment of inflammatory disorders

“…The cells, with spindle morphology, self-renewal ability, and multiple differentiation potential, isolated from pulp tissue, are called DPSCs. During infection and injury, DPSCs enhance their proliferation and differentiation ability and stimulate the migration of pulp progenitor cells to the site, where they generate a protective layer to protect and repair dentin [ 111 – 113 ]. Therefore, their potential therapeutic role in dental caries and other oral diseases has attracted increasing attention from researchers.…”

Maternal Supraphysiological Hypercholesterolemia Is Accompanied by Shifts in the Composition and Anti-Atherogenic Functions of Maternal HDL along with Maternal Cardiovascular Risk Markers at Term of Pregnancy

“…Interleukin-1β (IL-1β), IL-6, IL-8, IL-10, IL-12p70, and tumor necrosis factor (TNF) levels were determined in maternal total serum from MPH and MSPH women by flow cytometry using the Cytometric Bead Array (CBA) Human Inflammatory Cytokine Kit (BD, Biosciences, Franklin Lakes, NJ, USA), as described [23]. The results were expressed as the mean fluorescence intensity (MFI).…”