The immunomodulatory functions of chromogranin A-derived peptide pancreastatin

Ioannidis, Melina; Mahata, Sushil K.; Bogaart, Geert van den

doi:10.1016/j.peptides.2022.170893

Cited by 4 publications

(4 citation statements)

References 142 publications

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“…[ 69 ] Immunomodulatory peptides can act on lymphocyte proliferation and natural killer (NK) cell activity, although this knowledge is not fully understood. [ 70 ] Peptide immunomodulatory functions have been studied, such as the ability to influence immune cells by the pancreastatin peptide [ 71 ] and the significant immunomodulatory potential of antibacterial peptides isolated from the buffalo serum. [ 72 ] The presence of hydrophobic amino acids in the amino and carboxyl‐terminal regions and the presence of branched‐chain amino acids are indicated as important characteristics in immunomodulatory peptides.…”

Section: Resultsmentioning

confidence: 99%

Theoretical study of CHYMO32 peptide obtained by in silico fragmentation of the escapin protein isolated from marine hare Aplysia californica: A prediction for antimicrobial activity

Cardoso

Boeira

2023

Peptide Science

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The scenario involving the alarming growth of bacterial resistance has never been more worrying. Increasingly selective and sophisticated bacterial strains resistant to traditional antibiotics are a threat to the health system worldwide. Therefore, antimicrobial peptides (AMPs) represent a promising path in the fight against multidrug‐resistant pathogens. Here, using an in silico methodology, employing robust software, the present study aims to analyze the chymo32 peptide, obtained by enzymatic fragmentation of the escapin protein to test its possible antibacterial effects, correlating them with their physical–chemical nature. In this study, we used in silico predictions such as structural prediction, physicochemical properties, hemolytic activity, and prediction of activity for immunomodulation. Among the 378 peptide fragments obtained from the original protein, chymo32 was the only peptide selected in the field of screenings involving sequence length, cationicity, hydrophobicity, and prediction of antibacterial activity. The physical–chemical properties of chymo32 are promising, as well as its prediction as AMP. The immunomodulation predictions showed no immunogenic potential, which indicates greater safety in the posterior steps in vitro, also highlighting the absence of hemolytic activity, one of the main problems associated with AMPs in the therapeutic clinic.

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Section: Resultsmentioning

confidence: 99%

Theoretical study of CHYMO32 peptide obtained by in silico fragmentation of the escapin protein isolated from marine hare Aplysia californica: A prediction for antimicrobial activity

Cardoso

Boeira

2023

Peptide Science

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“…CgA undergoes various post-translational modifications in different cells and tissues, including phosphorylation, sulphation, glycosylation, and proteolytic cleavage [2,4]. Intra-granular and/or extra-cellular proteolytic enzymes, such as furin, cathepsin L, prohormone convertase 1 and 2, thrombin and plasmin, can cleave the full-length CgA precursor (CgA 1-439 ) at different sites to generate various biologically active fragments involved in the regulation of the innate immunity [5][6][7][8], cardiovascular system [9][10][11][12], metabolism [13][14][15], angiogenesis [16][17][18], tissue repair [19] and tumor growth [14,20,21]. These fragments include N-terminal large polypeptide fragments (e.g., CgA 1-373 ) [17], as well as shorter fragments, such as CgA 1-76 (vasostatin-1) [9], CgA 79-113 (vasoconstrictive-inhibitory factor) [22],…”

Section: Introductionmentioning

confidence: 99%

Neuropilin-1 and Integrins as Receptors for Chromogranin A-Derived Peptides

2022

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Human chromogranin A (CgA), a 439 residue-long member of the “granin” secretory protein family, is the precursor of several peptides and polypeptides involved in the regulation of the innate immunity, cardiovascular system, metabolism, angiogenesis, tissue repair, and tumor growth. Despite the many biological activities observed in experimental and preclinical models for CgA and its most investigated fragments (vasostatin-I and catestatin), limited information is available on the receptor mechanisms underlying these effects. The interaction of vasostatin-1 with membrane phospholipids and the binding of catestatin to nicotinic and b2-adrenergic receptors have been proposed as important mechanisms for some of their effects on the cardiovascular and sympathoadrenal systems. Recent studies have shown that neuropilin-1 and certain integrins may also work as high-affinity receptors for CgA, vasostatin-1 and other fragments. In this case, we review the results of these studies and discuss the structural requirements for the interactions of CgA-related peptides with neuropilin-1 and integrins, their biological effects, their mechanisms, and the potential exploitation of compounds that target these ligand-receptor systems for cancer diagnosis and therapy. The results obtained so far suggest that integrins (particularly the integrin avb6) and neuropilin-1 are important receptors that mediate relevant pathophysiological functions of CgA and CgA fragments in angiogenesis, wound healing, and tumor growth, and that these interactions may represent important targets for cancer imaging and therapy.

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“…Some of the products obtained after CgA degradation alter hormonal functions in autocrine and paracrine ways [9,18].Recent studies [13,15,16,17,19,20,23] have shown that CgA and some of its breakdown products, namely (pancreastatin, catestatin, vasostatin 1, WE-14) play an important role in the pathogenesis of various forms of diabetes [4,6,14,18,24], but the exact mechanisms of their action are still unclear. Several studies [12,17,20] have reported higher levels of pancreastatin and WE-14 in patients with type 1 and type 2 diabetes [12].According to a number of authors [1,11,13,15,18], pancreastatin, catestatin, and vasostatin play an important role in metabolic syndromes, namely obesity, diabetes, and others. Pancreastatin affects insulin [4,17,23], weakening insulin sensitivity and enhances inflammatory processes, while pancreastatin inhibitory peptide (PSTi8) improves insulin sensitivity and regulates glucose homeostasis [6,17,24].…”

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confidence: 99%

“…According to a number of authors [1,11,13,15,18], pancreastatin, catestatin, and vasostatin play an important role in metabolic syndromes, namely obesity, diabetes, and others. Pancreastatin affects insulin [4,17,23], weakening insulin sensitivity and enhances inflammatory processes, while pancreastatin inhibitory peptide (PSTi8) improves insulin sensitivity and regulates glucose homeostasis [6,17,24].…”

mentioning

confidence: 99%

Expression of Chromogranin (Cga) in Rat Pancreas During Alloxan Diabetes

MACHAVARIANI,

LATSABIDZE,

DGEBUADZE

et al. 2024

jecm

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Introduction. Chromogranin A (CgA) lipoprotein is one of the important representatives of the group of granins. CgA is found in neuroendocrine, endocrine, and nerve cells [2,21]. CgA is a granulogenic factor that regulates secretory functions in tissues. CgA participates in the generation of secretory granules, and its breakdown products are biologically active peptides [4,8,21]. CgA helps regulate secretion in granules and produces several degradation products after secretion. Some of the products obtained after CgA degradation alter hormonal functions in autocrine and paracrine ways [9,18].Recent studies [13,15,16,17,19,20,23] have shown that CgA and some of its breakdown products, namely (pancreastatin, catestatin, vasostatin 1, WE-14) play an important role in the pathogenesis of various forms of diabetes [4,6,14,18,24], but the exact mechanisms of their action are still unclear. Several studies [12,17,20] have reported higher levels of pancreastatin and WE-14 in patients with type 1 and type 2 diabetes [12].According to a number of authors [1,11,13,15,18], pancreastatin, catestatin, and vasostatin play an important role in metabolic syndromes, namely obesity, diabetes, and others. Pancreastatin affects insulin [4,17,23], weakening insulin sensitivity and enhances inflammatory processes, while pancreastatin inhibitory peptide (PSTi8) improves insulin sensitivity and regulates glucose homeostasis [6,17,24]. In contrast, catestatin CST increases insulin sensitivity and reduces inflammatory reactions. According to a number of studies [12,17,20,23,24] pancreastatin and catestatin regulate pancreatic β-cell insulin secretion and glucose metabolism. The metabolic effect of the peptide fragments obtained after CgA cleavage allows us to speculate on their possible participation in the pathophysiology of diabetes and to use it as a

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The immunomodulatory functions of chromogranin A-derived peptide pancreastatin

Cited by 4 publications

References 142 publications

Theoretical study of CHYMO32 peptide obtained by in silico fragmentation of the escapin protein isolated from marine hare Aplysia californica: A prediction for antimicrobial activity

Theoretical study of CHYMO32 peptide obtained by in silico fragmentation of the escapin protein isolated from marine hare Aplysia californica: A prediction for antimicrobial activity

Neuropilin-1 and Integrins as Receptors for Chromogranin A-Derived Peptides

Expression of Chromogranin (Cga) in Rat Pancreas During Alloxan Diabetes

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