The immunoreaction and antioxidant capacity of juvenile blunt snout bream (Megalobrama amblycephala) involves the PI3K/Akt/Nrf2 and NF-κB signal pathways in response to dietary methionine levels

Ji, Ke; Liang, Hualiang; Ren, Mingchun; Ge, Xianping; Mi, Haifeng; Pan, Liangkun; Yu, Huili

doi:10.1016/j.fsi.2020.07.005

Cited by 34 publications

(23 citation statements)

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“…In view of the dietary Met requirement of juvenile blunt snout bream determined by Liao et al [27], three isonitrogenous and isoenergetic feeds with the followed graded dietary Met levels were formulated: 0.40% (control), 0.84% (optimal) and 1.28% (high). The composition of the basal feed was shown in Table 1, and glycine was used to balance Met supplementation, as mentioned in our previous study [28]. The amino acid contents of the experimental diets are the same as those shown in the previous study by Ji et al [28].…”

Section: Experimental Design and Dietsmentioning

confidence: 99%

“…The composition of the basal feed was shown in Table 1, and glycine was used to balance Met supplementation, as mentioned in our previous study [28]. The amino acid contents of the experimental diets are the same as those shown in the previous study by Ji et al [28]. As described in our previous study [27], the pellet diets were processed by F-26 (II) (South China University of Technology, China), air-dried, and nally stored in a refrigerator at -20°C until feeding.…”

Section: Experimental Design and Dietsmentioning

confidence: 99%

“…Table 1 Ingredient composition of basal diets (% dry basis) Ingredients (%) Fish meal 1 2 Rapeseed meal 1 12 Soybean meal 1 30 Wheat meal 1 28 Rice meal 1 12. Note: the basal diet in this experiment was referred to our previous study (Ji et al, 2020). 1 The composition of sh meal, rapeseed meal, soybean meal, wheat meal, and rice meal as showed in our previous study (Ji et al, 2020).…”

Section: Experimental Design and Dietsmentioning

confidence: 99%

“…Protein sources obtained from Wuxi Tongwei feedstuffs Co., Ltd, Wuxi, China. 2 The details of amino acid premix as showed in our previous study (Ji et al, 2020). Amino acids obtained from Feeer Co., LTD (Shanghai, China).…”

Section: Experimental Design and Dietsmentioning

confidence: 99%

“…Blunt snout bream, Megalobrama amblycephala, is the main freshwater aquaculture species in China. Our previous studies have con rmed the dietary Met requirement and studied the immunity and antioxidant capacity of dietary methionine on blunt snout bream [27,28]. However, the information related to nutrient metabolism changes response to dietary Met in this species is still unknown.…”

Section: Introductionmentioning

confidence: 99%

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Tissue-specific Nutrient Metabolism in the Liver and Muscle of Juvenile Blunt Snout Bream (Megalobrama Amblycephala) in Response to Dietary Methionine Levels

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Liang

Ren

et al. 2021

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BackgroundMethionine is an essential amino acid, that affects the metabolism of protein, lipid and glucose. However, the metabolic polytrophic response in the liver and muscle of juvenile Megalobrama amblycephala to dietary methionine levels is unclear.ResultsThe 0.84% methionine diet significantly improved the growth performance compared with the 0.40% diet. Dietary methionine levels had no marked effects on plasma parameters or whole body composition of juveniles. The protein levels of phospho-phosphatidylinositol 3-kinase, protein kinase B, phospho-eukaryotic initiation factor 4E binding protein-1 (p-4E-BP1), 4E-BP1 and ribosomal protein S6 kinase 1, in the liver of fish fed the 0.84% diet were higher than those in fish fed the 0.40% diet. While in muscle, these proteins showed the opposite trend. The mRNA levels of the muscular lipid synthesis associated genes: sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthetase (FAS) and acetyl-CoA carboxylase (ACC), were significantly upregulated by the 1.28% methionine diet; while hepatic SREBP1, FAS and ACC mRNA expression levels were increased by 0.40% methionine. In addition, 1.28% dietary methionine significantly induced fatty acid β-oxidation and lipolysis of the liver and muscle via increased carnitine palmitoyl transferase 1, peroxisome proliferator activated receptor alpha, lipoprotein lipase and lipase expression levels. Compared with 0.40% dietary methionine, 1.28% methionine enhanced the mRNA levels of the hepatic gluconeogenesis related genes phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase, and the muscular glycolysis related genes phosphofructokinase (PFK) and pyruvate kinase (PK). The mRNA expression levels of hepatic PFK, PK and glucokinase were markedly upregulated by the 0.84% methionine diet compared with the 1.28% diet. In addition, muscular PEPCK and glycogen synthase, and hepatic glucose transporters 2 mRNA levels were induced by 1.28% methionine. ConclusionThe study showed that optimal methionine levels could enhance the growth of juvenile Megalobrama amblycephala, and the nutrient metabolism response to dietary methionine in the liver and muscle was tissue-specific.

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Section: Experimental Design and Dietsmentioning

confidence: 99%