The immunoregulatory effect of sulfated Echinacea purpurea polysaccharide on chicken bone marrow-derived dendritic cells

Yao, Lili; Bai, Lin; Tan, Ying; Sun, Jiaqi; Qu, Qian; Shi, Dayou; Guo, Shining; Liu, Cui

doi:10.1016/j.ijbiomac.2019.08.028

Cited by 27 publications

(14 citation statements)

References 43 publications

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“…Thus, the results presented in this study did not originate from a heterogenous population with distinct cell types as observed for the murine BM-DC cultures [ 46 ]. The overall phenotype of the BM-DCs derived using the approach employed in this study are therefore comparable to previous reports [ 47 , 48 , 49 ]. Mannan- and pustulan- treated BM-DCs increased MHCII surface expression ( Figure 1 A) and the expression of proinflammatory cytokines ( Figure 2 ).…”

Section: Discussionsupporting

confidence: 89%

Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4+ T Cell Recall Response to Infectious Bronchitis Virus

Larsen

Guldbrandtsen²,

Christensen

et al. 2020

Vaccines

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Infectious bronchitis virus (IBV) is a highly contagious avian coronavirus. IBV causes substantial worldwide economic losses in the poultry industry. Vaccination with live-attenuated viral vaccines, therefore, are of critical importance. Live-attenuated viral vaccines, however, exhibit the potential for reversion to virulence and recombination with virulent field strains. Therefore, alternatives such as subunit vaccines are needed together with the identification of suitable adjuvants, as subunit vaccines are less immunogenic than live-attenuated vaccines. Several glycan-based adjuvants directly targeting mammalian C-type lectin receptors were assessed in vitro using chicken bone marrow-derived dendritic cells (BM-DCs). The β-1-6-glucan, pustulan, induced an up-regulation of MHC class II (MHCII) cell surface expression, potentiated a strong proinflammatory cytokine response, and increased endocytosis in a cation-dependent manner. Ex vivo co-culture of peripheral blood monocytes from IBV-immunised chickens, and BM-DCs pulsed with pustulan-adjuvanted recombinant IBV N protein (rN), induced a strong recall response. Pustulan-adjuvanted rN induced a significantly higher CD4+ blast percentage compared to either rN, pustulan or media. However, the CD8+ and TCRγδ+ blast percentage were significantly lower with pustulan-adjuvanted rN compared to pustulan or media. Thus, pustulan enhanced the efficacy of MHCII antigen presentation, but apparently not the cross-presentation on MHCI. In conclusion, we found an immunopotentiating effect of pustulan in vitro using chicken BM-DCs. Thus, future in vivo studies might show pustulan as a promising glycan-based adjuvant for use in the poultry industry to contain the spread of coronaviridiae as well as of other avian viral pathogens.

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Section: Discussionsupporting

confidence: 89%

Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4+ T Cell Recall Response to Infectious Bronchitis Virus

Larsen

Guldbrandtsen²,

Christensen

et al. 2020

Vaccines

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“… DURATION: 5 days Negative control: no treatment No change in production: IL-2 and IL-10 3 Woelkart, 2006 [ 124 ] Institute of pharmaceutical sciences, department of pharmacognosy Blood samples LPS 100 pg mL + E51:F51 E.purpurea tincture (Echinaforce ) or tablet E. purpurea tincture containing 0.018 mg/mL of dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides and 1 E.purpurea tablet is 0.006 mg DURATION: 23 h Alcohol or lactose Decreased production: IL-8 and TNF-α No change in production: IL-6 3 Wu, 2009 [ 125 ] PolinaceaTM was donated by Indena s.p.a.; MiUR (PRIN 05) and Università degli Studi della Tuscia, and the Asia Link Project ‘‘Organic Farming: ethical, economic, technical and scientific aspects in a global perspective Peripheral blood mononuclear cells (from six healthy Holstein heifers) ConA (1 μg/mL) E. angustifolia Hydroethanolic root extract called Polinacea donated by Indena s.p.a. (Settala, Milan, Italy). Doses: 0, 6.3, 20, 60, and 180 μg/mL DURATION: 72 h Negative control: no stimulation and no treatment No change in secretion: IFN-γ 3 Yang, 2018 [ 126 ] State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University Spleen lymphocytes ConA (100 μg/mL) Tetraploid (CPE4) (85.51% crude polysaccharide) and diploid (CPE2) E. purpurea (44.65% crude polysaccharide) 0.5–0.0039 mg/mL DURATION: 48 h 10 μ g/mL ConA Increased production: IFN-γ, IL-2, TNF-α 3 Yao, 2019 [ 127 ] College of Veterinary Medicine, South China Agricultural University Chicken bone marrow-derived dendritic cells 5 μg/mL LPS E. purpurea polysaccharide (EPP) and sulfated EPP (sEPP) ...…”

Section: Resultsmentioning

confidence: 99%

A systematic review on the effects of Echinacea supplementation on cytokine levels: Is there a role in COVID-19?

et al. 2021

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COVID-19 is the respiratory illness caused by the novel coronavirus, SARS-CoV-2. Cytokine storm appears to be a factor in COVID-19 mortality. Echinacea species have been used historically for immune modulation. A previous rapid review suggested that Echinacea supplementation may decrease the levels of pro-inflammatory cytokines involved in cytokine storm. The objective of the present systematic review was to identify all research that has assessed changes in levels of cytokines relevant to cytokine storm in response to administration of Echinacea supplementation. The following databases were searched: Medline (Ovid), AMED (Ovid), CINAHL (EBSCO), EMBASE (Ovid). Title and abstract screening, full text screening, and data extraction were completed in duplicate using a piloted extraction template. Risk of bias assessment was completed. Qualitative analysis was used to assess for trends in cytokine level changes. The search identified 279 unique publications. After full text screening, 105 studies met criteria for inclusion including 13 human studies, 24 animal studies, and 71 in vitro or ex vivo studies. The data suggest that Echinacea supplementation may be associated with a decrease in the pro-inflammatory cytokines IL-6, IL-8, and TNF, as well as an increase in the anti-inflammatory cytokine IL-10. The risk of bias in the included studies was generally high. While there is currently no substantive research on the therapeutic effects of Echinacea in the management of either cytokine storm or COVID-19, the present evidence related to the herb's impact on cytokine levels suggests that further research may be warranted in the form of a clinical trial involving patients with COVID-19.

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“…showed that sulfation of a polysaccharide from Echinacea purpurea enhances its effect on phagocytosis as well as NO, IL‐2, and IFN‐γ production by DCs and also inhibits production of anti‐inflammatory IL‐4 and IL‐10. [ 197 ] It was shown that sulfated K5 with a high degree of O‐ sulfation (DS = 0.3) enhances the apoptotic induction by NK cells. [ 196 ]…”

Section: Biological Properties and Biomedical Applications Of Espsmentioning

confidence: 99%

“…[191] In an in vivo experiment, mice were treated with an intragastrical-administered ESP prepared from sulfation of another fungal polysaccharide from Polyporus albicans mycelia, which demonstrated that the stimulatory effect on NO and ROS production by macrophages was significantly enhanced in a dose-and DS-dependent manner. [195] In addition to macrophages, studies have found that sulfation of naturally nonsulfated polysaccharides enhances their immunoactivating effect on other cell types involved in innate immunity, including DCs [197] and NK cells. [196] Yao et al showed that sulfation of a polysaccharide from Echinacea purpurea enhances its effect on phagocytosis as well as NO, IL-2, and IFN-γ production by DCs and also inhibits production of anti-inflammatory IL-4 and IL-10.…”

Section: Immunoactivationmentioning

confidence: 99%

“…[196] Yao et al showed that sulfation of a polysaccharide from Echinacea purpurea enhances its effect on phagocytosis as well as NO, IL-2, and IFN-γ production by DCs and also inhibits production of anti-inflammatory IL-4 and IL-10. [197] It was shown that sulfated K5 with a high degree of O-sulfation (DS = 0.3) enhances the apoptotic induction by NK cells. [196] Surface receptors on macrophages known as pattern recognition receptors can recognize foreign ligands during initial phases of the immune response.…”

Section: Immunoactivationmentioning

confidence: 99%

See 1 more Smart Citation

Engineered Sulfated Polysaccharides for Biomedical Applications

Arlov

Rütsche

Korayem

et al. 2021

Adv Funct Materials

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Sulfated polysaccharides are ubiquitous in living systems and have central roles in biological functions such as organism development, cell proliferation and differentiation, cellular communication, tissue homeostasis, and host defense. Engineered sulfated polysaccharides (ESPs) are structural derivatives not found in nature but generated through chemical and enzymatic modification of natural polysaccharides, as well as chemically synthesized oligo‐ and polysaccharides. ESPs exhibit novel and augmented biological properties compared with their unmodified counterparts, mainly through facilitating interactions with other macromolecules. These interactions are closely linked to their sulfation patterns and backbone structures, providing a means to fine‐tune biological properties and characterize structural–functional relationships by employing well‐characterized polysaccharides and strategies for regioselective modification. The following review provides a comprehensive overview of the synthesis and characterization of ESPs and of their biological properties. Through the pioneering research presented here, key emerging application areas for ESPs, which can lead to novel breakthroughs in biomedical research and clinical treatments, are highlighted.

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The immunoregulatory effect of sulfated Echinacea purpurea polysaccharide on chicken bone marrow-derived dendritic cells

Cited by 27 publications

References 43 publications

Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4+ T Cell Recall Response to Infectious Bronchitis Virus

Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4+ T Cell Recall Response to Infectious Bronchitis Virus

A systematic review on the effects of Echinacea supplementation on cytokine levels: Is there a role in COVID-19?

Engineered Sulfated Polysaccharides for Biomedical Applications

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