Chemical properties (total and available P concentration; oxidizable C concentration; available K, Na, and Ca concentration; and pH) were quantified for 33 nests of the ant Myrmica ruginodis and in surrounding soil in young spruce forest stands. All properties, except total P, were significantly higher in the nests than in the surrounding soil. Total P was not higher in nests than in surrounding soil across all nests because nests had higher total P than surrounding soil if the soil contained low concentrations of total P but nests had lower total P than surrounding soil if the soil contained high concentrations of total P. The effect of nests on total P in the surrounding soil corresponded with effects of nests on oxidizable carbon (an indicator of organic matter) in the surrounding soil (concentrations of oxidizable carbon and total P were closely correlated). Available P concentrations were much higher in nests than in surrounding soil. Overall, the results indicated that two main processes explain the chemical changes of soil in the ant nests: (i) mixing due to excavation of deeper soil layers and (ii) deposition of excreta and food residues. The effect of soil mixing (whereby ants transport mineral soil from deeper layers to layers near the surface) is more pronounced in soils with high organic content near the surface because mixing increases the proportion of mineral soil in the nest while decreasing the proportion of organic matter and the concentration of total P.