The impact of antibiotic exposure in water and zebrafish gut microbiomes: A 16S rRNA gene-based metagenomic analysis

Almeida, Ana Rita; Alves, Marta; Domingues, Inês; Henriques, Isabel

doi:10.1016/j.ecoenv.2019.109771

Cited by 57 publications

(28 citation statements)

References 39 publications

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“…6A), which can explain this observed heterogeneity. Divergence between replicates is a frequently observed phenomenon during cultivation of different species, in both batch and recirculation systems (Chun et al ., 2018; Almeida et al ., 2019; Li et al ., 2019; Dahle et al ., 2020), suggesting that stochastic assembly may be a widespread characteristic of aquaculture systems. Possible causes for this stochasticity may be the regime of the disturbances through water exchanges, feed addition, so on (Santillan et al ., 2018) or the eutrophication of the water (Yang et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Molecular studies on rearing water microbiomes have shown that the community composition of these systems is dynamic over time (Xiong et al ., 2014a,b; Zheng et al ., 2017; Yan et al ., 2020) and exhibits large variability across replicate cultivations (Schmidt et al ., 2017; Chun et al ., 2018; Almeida et al ., 2019; Li et al ., 2019; Dahle et al ., 2020). The water microbiome experiences frequent disturbances through the addition of live and dry feeds, probiotics and water exchanges, each of which carries with them their own microbiome.…”

Section: Introductionmentioning

confidence: 99%

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Rearing water microbiomes in white leg shrimp (Litopenaeus vannamei) larviculture assemble stochastically and are influenced by the microbiomes of live feed products

Heyse

Props

Kongnuan³

et al. 2020

Environmental Microbiology

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The development of effective management strategies to reduce the occurrence of diseases in aquaculture is hampered by the limited knowledge on the microbial ecology of these systems. In this study, the dynamics and dominant community assembly processes in the rearing water of Litopenaeus vannamei larviculture tanks were determined. Additionally, the contribution of peripheral microbiomes, such as those of live and dry feeds, to the rearing water microbiome were quantified. The community assembly in the hatchery rearing water over time was dominated by stochasticity, which explains the observed heterogeneity between replicate cultivations. The community undergoes two shifts that match with the dynamics of the algal abundances in the rearing water. Source tracking analysis revealed that 37% of all bacteria in the hatchery rearing water were introduced either by the live or dry feeds, or during water exchanges. The contribution of the microbiome from the algae was the largest, followed by that of the Artemia, the exchange water and the dry feeds. Our findings provide fundamental knowledge on the assembly processes and dynamics of rearing water microbiomes and illustrate the crucial role of these peripheral microbiomes in maintaining health-promoting rearing water microbiomes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rearing water microbiomes in white leg shrimp (Litopenaeus vannamei) larviculture assemble stochastically and are influenced by the microbiomes of live feed products

Heyse

Props

Kongnuan³

et al. 2020

Environmental Microbiology

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show abstract

“…At the family level, 0.2% β-glucan supplementation significantly decreased the abundance of Thermoactinomycetacae, Caldilineaceae, Solirubrobacteraceae, Microbacteriaceae and Phyllobacteriaceae in the intestine of tilapia as compared with those in the freshwater group. The Caldilineaceae abundance can be inhibited by resveratrol and oxytetracycline intake in tilapia and zebrafish, respectively [73][74][75]. Meanwhile, resveratrol can also increase the abundance of Firmicutes, just like β-glucan in tilapia [74].…”

Section: Discussionmentioning

confidence: 99%

Recovery from Hypersaline-Stress-Induced Immunity Damage and Intestinal-Microbiota Changes through Dietary β-glucan Supplementation in Nile tilapia (Oreochromis niloticus)

Suo

Wang

et al. 2020

Animals

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Long-term exposure to hyperosmotic environments can induce severe immune damage and increase risk in tilapia breeding. As an effective immunoregulator, β-glucan has attracted extensive attention in nutritional research and given rise to high expectations of improving health status and alleviating organismal damage in tilapia, Oreochromis niloticus, in brackish water. In this study, an 8-week cultivation experiment was conducted on tilapia fed a basal diet or diets with β-glucan supplementation in freshwater (control) and brackish water. Growth performance, hematological aspects, immune cytokine expression, and the intestinal microbiota of tilapia were analyzed. The results indicated that supplementation with β-glucan significantly reduced the enlarged spleen of tilapia resulting from hypersaline stress. Tilapia fed β-glucan showed significantly-greater decreases in the red blood cell count, hematocrit, red cell distribution width, platelet count, and plateletcrit than those fed the basal diet. β-glucan significantly decreased the high expression of immune-related genes in the spleen induced by hyperosmotic stress. In the intestine, the high migration inhibitory factor-2 (MIF-2) and IL-1β gene expression induced by hypersaline stress was significantly reduced. β-glucan supplementation also significantly increased the abundance of beneficial microbiota such as Lactobacillus, Phycicoccus, and Rikenellaceae. Therefore, dietary β-glucan supplementation can significantly reduce spleen enlargement and improve immune function in tilapia in brackish water. β-glucan intake can also optimize the intestinal microbiota of tilapia in brackish water and improve fish health.

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“…For these reasons, antibiotics are still widely used to control disease in aquaculture. Several studies have reported the negative impacts of antibiotic use on sh, which include behavioral changes [22], microbial diversity decrease [7,22,23], increased susceptibility to secondary infections [24], changes in predicted microbial function [23] and mortality [25]. Flumequine is a uoroquinolone antibiotic active against Gram-negative bacteria and widely used in aquaculture [26].…”

Section: Introductionmentioning

confidence: 99%

Monitoring disease and antibiotic treatment in the skin microbiota of farmed seabass fingerlings

Rosado¹,

Pérez‐Losada²,

Severino

et al. 2021

Preprint

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The microbiota of fish skin is the primary barrier against disease; however, it is highly dynamic being modulated by several factors. In fish aquaculture, disease outbreaks occur mainly during early-life stages with high associated losses. Antibiotic treatments sometimes remain as the best option to control bacterial diseases, despite many reported negative impacts of its use on fish and associated microbiota. Notwithstanding, studies monitoring the effects of disease and antibiotic treatment on the microbiota of fingerlings are scarce. We used a 16S rRNA metataxonomic approach to assess the impact of a mixed infection with Photobacterium damselae ssp. piscicida and Vibrio harveyi and subsequent antibiotic treatment with flumequine, on the skin microbiota of farmed seabass Dicentrarchus labrax fingerlings. Both disease and antibiotic treatment led to a significant increase in bacterial diversity and core microbial communities and impacted microbial structure. Dysbiosis was confirmed by changes in the abundance of potential pathogenic and opportunistic bacterial taxa. Skin bacterial metabolic function was also significantly affected by flumequine administration, suggesting a detriment to fish skin health. Our results add to an increasing body of literature, showing how fish microbiome response to disease and antibiotics is not easily predicted.

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The impact of antibiotic exposure in water and zebrafish gut microbiomes: A 16S rRNA gene-based metagenomic analysis

Cited by 57 publications

References 39 publications

Rearing water microbiomes in white leg shrimp (Litopenaeus vannamei) larviculture assemble stochastically and are influenced by the microbiomes of live feed products

Rearing water microbiomes in white leg shrimp (Litopenaeus vannamei) larviculture assemble stochastically and are influenced by the microbiomes of live feed products

Recovery from Hypersaline-Stress-Induced Immunity Damage and Intestinal-Microbiota Changes through Dietary β-glucan Supplementation in Nile tilapia (Oreochromis niloticus)

Monitoring disease and antibiotic treatment in the skin microbiota of farmed seabass fingerlings

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