The Impact of Biofilm Formation on Wound Healing

Mendoza, Rafael A.; Hsieh, Ji-Cheng; Galiano, Robert D.

doi:10.5772/intechopen.85020

Cited by 31 publications

(27 citation statements)

References 37 publications

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“…These molecules are sensed by the other local population, triggering, for instance, changes in the expression and regulation of genes, virulence, microbial competence, and also antibiotic resistance of the cells [71,72]. Also, the EPS matrix serves as a physical barrier to immune cells, limiting leukocyte and bactericidal product penetration and avoiding cell phagocytosis, resulting in collateral tissue damage and chronic wound inflammation; both of which delay healing [73]. Besides cell protection by the EPS matrix, biofilm cells also escape the immune system response by genetically activating the quorum sense response regulators, genetic switches, or suppressors [74].…”

Section: Biofilmsmentioning

confidence: 99%

“…Besides cell protection by the EPS matrix, biofilm cells also escape the immune system response by genetically activating the quorum sense response regulators, genetic switches, or suppressors [74]. Cell survival in biofilm communities profoundly challenges the treatment of biofilm-related infections, including the treatment of chronic wound infections [56,73].…”

Section: Biofilmsmentioning

confidence: 99%

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Bacteriophages for Chronic Wound Treatment: From Traditional to Novel Delivery Systems

Pinto

Cerqueira

Bañobre-Lópes

et al. 2020

Viruses

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The treatment and management of chronic wounds presents a massive financial burden for global health care systems, with significant and disturbing consequences for the patients affected. These wounds remain challenging to treat, reduce the patients' life quality, and are responsible for a high percentage of limb amputations and many premature deaths. The presence of bacterial biofilms hampers chronic wound therapy due to the high tolerance of biofilm cells to many firstand second-line antibiotics. Due to the appearance of antibiotic-resistant and multidrug-resistant pathogens in these types of wounds, the research for alternative and complementary therapeutic approaches has increased. Bacteriophage (phage) therapy, discovered in the early 1900s, has been revived in the last few decades due to its antibacterial efficacy against antibiotic-resistant clinical isolates. Its use in the treatment of non-healing wounds has shown promising outcomes. In this review, we focus on the societal problems of chronic wounds, describe both the history and ongoing clinical trials of chronic wound-related treatments, and also outline experiments carried out for efficacy evaluation with different phage-host systems using in vitro, ex vivo, and in vivo animal models. We also describe the modern and most recent delivery systems developed for the incorporation of phages for species-targeted antibacterial control while protecting them upon exposure to harsh conditions, increasing the shelf life and facilitating storage of phage-based products. In this review, we also highlight the advances in phage therapy regulation.

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Section: Biofilmsmentioning

confidence: 99%

Section: Biofilmsmentioning

confidence: 99%

Bacteriophages for Chronic Wound Treatment: From Traditional to Novel Delivery Systems

Pinto

Cerqueira

Bañobre-Lópes

et al. 2020

Viruses

View full text Add to dashboard Cite

show abstract

“…Additionally, significant delay in wound healing cause of P. aeruginosa biofilms inhibit neutrophil movement [41]. So the coordinated control of the production of virulence and antibiotic resistance factors and the ability to adapt to various environmental changes is a likely and important reason that P. aeruginosa is a successful and common pathogen [42].…”

Section: Bacterial Species Of Dfu Associated With Wagner's Grades Systemmentioning

confidence: 99%

Assessment role of phagocytic neutrophil cells among different Wagner’s grades of diabetic foot ulcers infections

Bin-Hameed¹,

Baras²

2020

Preprint

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Background: Foot ulcers complications in diabetes mellitus (DM) patients are one of the significant medical problems and an economic burden. The aim of this study is to assessment role of phagocytic neutrophil cells and its relationship with the incidence of diabetic foot ulcers (DFU) infection of diabetic patients. A total of 60 venipuncture blood samples collected from diabetes mellitus, diabetic foot ulcer patients and healthy persons as control group. 20 swabs from the DFU patients were collected and processed for culture and susceptibility test after the ulcers classified according to Wagner’s grades system. Phagocytic cells activity test was performed to determine the efficiency of phagocytic neutrophil cells in diabetic patients. Results: Gram positive bacteria were the most prevalent in the DFU patients 57.1% with statistical significant relationship between the type of bacteria and grades of Wagner’s classification followed by Gram negative bacteria in high grades of ulcers. Wagner’s ulcers grade 1 and 2 were the most prevalence in DFU patients 30%. There was a weak negative correlation between the efficiency of phagocytic neutrophil cells activity and grade ulcers classified (r = -0.323). Amikacin and ciprofloxacin were the most effective antibiotics against 90.5% and 81% of the bacterial isolates respectively, whereas ampicillin, cefepime and cefadroxil were less effective antibiotics against the bacterial isolates.Conclusion: When the grade of ulcer increased, the bacterial resistance to antibiotics increased, and this was emphasis the correlation with prevalent of Gram negative bacteria in the high grade of ulcers with high resistance of antibiotics. In contrast, the grade of ulcer increased, the efficiency of phagocytic neutrophil cells decreased.

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“…Candida albicans) could colonize wounds and produce persistent infections [12]. A major concern in infected wounds is the formation of biofilms, which are multicellular communities of microbial species Materials 2020, 13, 1682 2 of 13 that manifest particular behaviour and increased tolerance to high amounts of antimicrobial drugs and also to the host's immune response [13].…”

Section: Introductionmentioning

confidence: 99%

Wound Dressings Coated with Silver Nanoparticles and Essential Oils for The Management of Wound Infections

et al. 2020

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Infection represents one of the major risk factors in persistent and difficult to treat wounds. This study focuses on developing antimicrobial wound dressings coated with silver nanoparticles, sodium alginate and different essential oils, to avoid wound infection and biofilm formation. The design of the wound dressings was done by the dip coating method. The characteristics of the developed materials were analysed by physicochemical (FT-IR, XRD, SEM, TEM) and biological (antimicrobial tests) approaches. The results demonstrated uniform silver nanoparticle formation on the substrate, and the developed nanomodified dressings were proven to have increased antimicrobial and antibiofilm potential. The developed wound dressings based on silver nanoparticles, sodium alginate and essential oils have real potential in treating infections, and can be investigated as an efficient alternative to antibiotics and topical preparations for wound management.

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The Impact of Biofilm Formation on Wound Healing

Cited by 31 publications

References 37 publications

Bacteriophages for Chronic Wound Treatment: From Traditional to Novel Delivery Systems

Bacteriophages for Chronic Wound Treatment: From Traditional to Novel Delivery Systems

Assessment role of phagocytic neutrophil cells among different Wagner’s grades of diabetic foot ulcers infections

Wound Dressings Coated with Silver Nanoparticles and Essential Oils for The Management of Wound Infections

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