The impact of biomechanics on corneal endothelium tissue engineering

Tsai, Meng-Chen; Daniels, Julie T.

doi:10.1016/j.exer.2021.108690

Cited by 6 publications

(8 citation statements)

References 105 publications

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“…Tissue engineering has developed substantially in recent years and has been used in multiple disciplines, including cell biology, [ 34 ] biomechanics, [ 35 ] and biomedical engineering. [ 36 ] The major focus of modern cell and tissue engineering research is to make use of the normal growth and differentiation of cells in a matrix to form new or replace damaged tissues or organs.…”

Section: Discussionmentioning

confidence: 99%

Organoid‐Loaded Cryomicroneedles for Biomimic Hair Regeneration

Zheng,

Yang,

Feng

et al. 2023

Adv Funct Materials

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Organoids have attracted extensive attention in the field of hair follicle (HF) tissue engineering and regeneration. However, HF organoid implantation faces a number of difficulties, such as low viability ratios, invasive trauma, and complicated procedures. To overcome these issues, a convenient and minimally invasive delivery approach with high efficiency and cell viability is required. Here, HF organoid‐loaded methacrylated gelatin‐cryomicroneedles (GelMA‐cryoMNs) that are capable of efficient transdermal delivery and can maintain the viability of the embedded organoids are developed. The GelMA‐cryoMNs are fabricated by a stepwise cryogenic molding process using a balanced ratio of cryopreservation agents and GelMA hydrogel. They can be inserted easily into porcine skin and melted without leaving residue after delivering the organoids. In vitro and in vivo, organoids delivered by the cryoMNs retain cell viability, self‐assembly, and differentiation ability. In mice, HF organoid‐loaded GelMA‐cryoMNs are delivered to the superficial dermis, resulting in subsequent HF development and the formation of an array of biomimetic hair tufts that broke through the skin surface. A promising strategy is provided here for biomimetic hair neogeneration that benefits from minimal invasion, low cost, and simple fabrication. The GelMA‐cryoMNs developed here could also have broader applications in tissue engineering and organ regeneration.

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Section: Discussionmentioning

confidence: 99%

Organoid‐Loaded Cryomicroneedles for Biomimic Hair Regeneration

Zheng,

Yang,

Feng

et al. 2023

Adv Funct Materials

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“…Integrin binding sites establish a connection between the cell surface and the ECM. The extracellular domains of integrins attach to matrix molecules such as laminin, nidogen, fibronectin, and collagen, as schematically shown in Figure 3 [ 53 ]. This evidence supports the idea that the corneal endothelium and the ECM have a strong structural connection.…”

Section: Materials For Producing Scaffolds Of Cecsmentioning

confidence: 99%

“…During this process, biophysical cues from the ECM can be transformed into intracellular biochemical signals that trigger responses from cells. Topographical cues can affect cell cytoskeletal configuration through the JNK-ERK1/2 and PI3K pathways [ 53 ]. The mean cell area of human CECs ranges from 332.3 ± 46.3 μm 2 to 390.59 ± 149.94 μm 2 , enabling cells to perceive topographical features ranging in size from nanometers to micrometers, such as those lying on fibrils [ 71 ].…”

Section: Materials For Producing Scaffolds Of Cecsmentioning

confidence: 99%

Research Progress of Polymer Biomaterials as Scaffolds for Corneal Endothelium Tissue Engineering

Luo

Zhao

et al. 2023

Nanomaterials

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Nowadays, treating corneal diseases arising from injury to the corneal endothelium necessitates donor tissue, but these corneas are extremely scarce. As a result, researchers are dedicating significant efforts to exploring alternative approaches that do not rely on donor tissues. Among these, creating a tissue-engineered scaffold on which corneal endothelial cells can be transplanted holds particular fascination. Numerous functional materials, encompassing natural, semi-synthetic, and synthetic polymers, have already been studied in this regard. In this review, we present a comprehensive overview of recent advancements in using polymer biomaterials as scaffolds for corneal endothelium tissue engineering. Initially, we analyze and present the key properties necessary for an effective corneal endothelial implant utilizing polymer biomaterials. Subsequently, we focus on various emerging biomaterials as scaffolds for corneal endothelium tissue engineering. We discuss their modifications (including natural and synthetic composites) and analyze the effect of micro- and nano-topological morphology on corneal endothelial scaffolds. Lastly, we highlight the challenges and prospects of these materials in corneal endothelium tissue engineering.

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“…Hydrophilic materials (e.g., gelatin, chitosan, and collagen) can support adhesion to the cornea while promoting cell adhesion without the use of additional adhesives [9,10]. Long-term adhesion between the scaffold and the cornea during the healing process is achieved through bioactive protein adsorption, mainly fibronectin, laminin, and collagen [11,12]. The shape of the scaffold has a similar curvature to the corneal stroma, which helps to adhere perfectly to the posterior surface of the cornea.…”

Section: Biocompatibilitymentioning

confidence: 99%

The Innovative Biomaterials and Technologies for Developing Corneal Endothelium Tissue Engineering Scaffolds: A Review and Prospect

Chi,

Yuan,

Xie

et al. 2023

Bioengineering

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Corneal transplantation is the only treatment for corneal endothelial blindness. However, there is an urgent need to find substitutes for corneal endothelium grafts due to the global shortage of donor corneas. An emerging research field focuses on the construction of scaffold-based corneal endothelium tissue engineering (CETE). Long-term success in CETE transplantation may be achieved by selecting the appropriate biomaterials as scaffolds of corneal endothelial cells and adding bioactive materials to promote cell activity. This article reviews the research progress of CETE biomaterials in the past 20 years, describes the key characteristics required for corneal endothelial scaffolds, and summarizes the types of materials that have been reported. Based on these, we list feasible improvement strategies for biomaterials innovation. In addition, we describe the improved techniques for the scaffolds’ surface topography and drug delivery system. Some promising technologies for constructing CETE are proposed. However, some questions have not been answered yet, and clinical trials and industrialization should be carried out with caution.

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The impact of biomechanics on corneal endothelium tissue engineering

Cited by 6 publications

References 105 publications

Organoid‐Loaded Cryomicroneedles for Biomimic Hair Regeneration

Organoid‐Loaded Cryomicroneedles for Biomimic Hair Regeneration

Research Progress of Polymer Biomaterials as Scaffolds for Corneal Endothelium Tissue Engineering

The Innovative Biomaterials and Technologies for Developing Corneal Endothelium Tissue Engineering Scaffolds: A Review and Prospect

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