The Impact of Biotic and Abiotic Stress Factors on Development of European Ash Tissue Cultures

Nawrot-Chorabik, Katarzyna; Sułkowska, Małgorzata; Osmenda, Małgorzata; Mohytych, Vasyl; Surówka, Ewa; Latowski, Dariusz

doi:10.3390/f13010059

Cited by 8 publications

(6 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite several fungal species being able to reduce avocado production, new organisms with phytopathogenic activity can be found under open field conditions [ 4 ]. The phytopathogenic behavior of these organisms is usually associated with specific climatic and soil features in which the avocado plants grow [ 5 ]. The state of Puebla is the sixth largest producer of avocado in Mexico and the northern highlands of this state are considered a new zone dedicated to the production of this fruit.…”

Section: Introductionmentioning

confidence: 99%

Improving the Shelf Life of Avocado Fruit against Clonostachys rosea with Chitosan Hybrid Films Containing Thyme Essential Oil

et al. 2022

View full text Add to dashboard Cite

Hass avocadoes are one of the most popular fruits consumed worldwide because of their nutritional and nutraceutical content. Nevertheless, these fruits are susceptible to phytopathogen attacks that decrease fruit quality during the postharvest period. Herein we present the results of the in situ fungistatic activity of four hybrid films (FT1–FT4) manufactured with chitosan and different concentrations of the essential oil of thyme (TvEO). The films were evaluated as biodegradable materials to prevent fruit decay triggered by Clonostachys rosea which is considered an emergent phytopathogen of this crop. The in situ fungistatic strength, spectroscopic properties (FT-IR), optical features (transmittance/opacity), and consistency obtained by microscopic analysis (SEM), indicated that the films FT3 and FT4 possessed the best physicochemical properties to protect Hass avocadoes against the soft rot produced by C. rosea. Avocadoes treated with the films FT3 and FT4 significantly (p < 0.01) conserved fruit firmness and nutritional composition (protein, fat, fiber, and reducing sugars) as well as the nutraceutical content (oleic, palmitoleic, linoleic, and palmitic acids) of infected avocados for 21 days. Our results validate the potential use of the films FT3 and FT4 to prevent the soft rot caused by C. rosea and to improve the shelf life of Hass avocadoes.

show abstract

Section: Introductionmentioning

confidence: 99%

Improving the Shelf Life of Avocado Fruit against Clonostachys rosea with Chitosan Hybrid Films Containing Thyme Essential Oil

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It has been reported that cadmium and lead show similar negative effects on callus tissue cultures and seedlings of common ash (F. excelsior) in laboratory conditions, and the toxic effect of lead on seedlings is slightly weaker than the toxic effect of cadmium (Nawrot-Chorabik et al 2022). Although there are no similar studies on narrow-leaved ash (F. angustifolia), it can be reasonably assumed that the effects of both heavy metals would be similar.…”

Section: Discussionmentioning

confidence: 98%

Effect of cadmium (Cd) and lead (Pb) soil contamination on the development of Hymenoscyphus fraxineus on Fraxinus excelsior and F. angustifolia seedlings

Vemić,

Popović,

Miletić

et al. 2023

iForest

View full text Add to dashboard Cite

Biogeosciences and Forestry Biogeosciences and Forestry Effect of cadmium (Cd) and lead (Pb) soil contamination on the development of Hymenoscyphus fraxineus on Fraxinus excelsior and F. angustifolia seedlings Aleksandar Vemić (1) , Vladan Popović (2) , Zoran Miletić (3) , Zlatan Radulović (4) , Ljubinko Rakonjac (3) , Aleksandar Lučić (2) In light of the increase of environmental pollution, we tested the effect of cadmium (Cd) and lead (Pb) soil contamination on ash dieback. The experiment included the inoculation of Hymenoscyphus fraxineus on Fraxinus excelsior and Fraxinus angustifolia seedlings growing on unpolluted soil, soil contaminated with cadmium (Cd), and soil contaminated with lead (Pb). At the end of the experiment, 173 days after soil contamination and 50 days since inoculation, all F. excelsior and F. angustifolia seedlings inoculated with H. fraxineus showed ash dieback symptoms in comparison to their control groups. However, both F. excelsior and F. angustifolia seedlings grown on contaminated soil had significantly increased necrotic lesions in comparison to the seedlings grown on uncontaminated soil. Our results showed for the first time that cadmium (Cd) and lead (Pb) soil contamination can significantly contribute to ash dieback and increase damage to F. excelsior and F. angustifolia seedlings.

show abstract

“…These studies are continued at the Department of Forest Ecosystem Protection. They refer to endophytic fungi as biotic stress factors, but are also extended to abiotic stress factors (heavy metals and salinity of the substrate) [53]. The setup is also useful in testing for production of biologically active metabolites, including compounds with potential medicinal applications.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Biocontrol Potential of Two Ash Endophytes against Hymenoscyphus fraxineus Using In Vitro Plant–Fungus Dual Cultures

Nawrot-Chorabik

Marcol-Rumak

Latowski

2021

Forests

View full text Add to dashboard Cite

Development of effective biocontrol procedures using ash endophytes to combat an ash pathogen Hymenoscyphus fraxineus would be an appropriate contribution to the ongoing effort to protect European ash stands against ash decline. In this study we investigated the biocontrol potential of two ash endophytes, Thielavia basicola and Minimidochium sp., against H. fraxineus using in vitro plant-fungus and fungus-fungus dual cultures approach in three biocontrol models. The tests aimed to determine whether the endophytes show antagonism toward Fraxinus excelsior and F. pennsylvanica, to assess the level of antagonism of the endophytes toward H. fraxineus and to identify potential secondary metabolites induced by the presence of H. fraxineus. The results that dual culture experiments modeled according to our design may be a very useful tool to precisely study biocontrol potential of fungi, i.e., without the impact of environmental factors. Such experiments also enable the selection of most resistant ash genotypes and rapid propagation, producing large numbers of pathogen-free seedlings. It should be noted, however, that both of the endophytes tested in the dual cultures strongly inhibited the growth of H. fraxineus. Their growth under the influence of callus/seedlings was also inhibited. Comparison of HPLC profiles showed that the presence of H. fraxineus in the post-culture medium induced the production of an unknown secondary metabolite in this species. Such results suggest that some of the plant–fungus combinations examined in this study may have potential to be developed as biocontrol methods, thus increasing the survivability of ash stands under natural conditions.

show abstract

The Impact of Biotic and Abiotic Stress Factors on Development of European Ash Tissue Cultures

Cited by 8 publications

References 65 publications

Improving the Shelf Life of Avocado Fruit against Clonostachys rosea with Chitosan Hybrid Films Containing Thyme Essential Oil

Improving the Shelf Life of Avocado Fruit against Clonostachys rosea with Chitosan Hybrid Films Containing Thyme Essential Oil

Effect of cadmium (Cd) and lead (Pb) soil contamination on the development of Hymenoscyphus fraxineus on Fraxinus excelsior and F. angustifolia seedlings

Investigation of the Biocontrol Potential of Two Ash Endophytes against Hymenoscyphus fraxineus Using In Vitro Plant–Fungus Dual Cultures

Contact Info

Product

Resources

About