The impact of Bt maize expressing the Cry1Ac protein on non-target arthropods

Xing, Yiming; Qin, Zifang; Feng, Mingyue; Li, Aomei; Zhang, Liu; Wang, Yang; Dong, Xin; Zhang, Yuxin; Tan, Shuqian; Shi, Wangpeng

doi:10.1007/s11356-018-4025-4

Cited by 11 publications

(9 citation statements)

References 25 publications

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“…Our results showed the Cry1Ab-Gc over-expression maize had no significantly negative impacts on arthropod diversity in the field. This result was consistent with previous studies, which proved that BT maize include Cry1Ab 49 , Cry1Ac 50 , 51 and Cry3Bb 52 did not affect the population dynamics and biodiversity of the non-target insect. Notably, in this study, we only conducted one-year research, so continuous and systematic research would be helpful to confirm the environmental safety of Cry1Ab-Gc protein release.…”

Section: Discussionsupporting

confidence: 93%

Engineered chimeric insecticidal crystalline protein improves resistance to lepidopteran insects in rice (Oryza sativa L.) and maize (Zea mays L.)

Liu

Han

Yang

et al. 2022

Sci Rep

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The insecticidal crystalline proteins (Crys) are a family of insect endotoxin functioning in crop protection. As insects keep evolving into tolerance to the existing Crys, it is necessary to discover new Cry proteins to overcome potential threatens. Crys possess three functional domains at their N-termini, and the most active region throughout evolution was found at the domain-III. We swapped domain-IIIs from various Cry proteins and generated seven chimeric proteins. All recombinants were expressed in Escherichia coli and their toxicity was assessed by dietary exposure assays. Three of the seven Crys exhibited a high toxicity to Asian corn borer over the controls. One of them, Cry1Ab-Gc, a chimeric Cry1Ab being replaced with the domain-III of Cry1Gc, showed the highest toxicity to rice stem borer when it was over-expressed in Oryza sativa. Furthermore, it was also transformed into maize, backcrossed into commercial maize inbred lines and then produced hybrid to evaluate their commercial value. Transgenic maize performed significant resistance to the Asian corn borer without affecting the yield. We further showed that this new protein did not have adverse effects on the environment. Our results indicated that domain III swapped of Crys could be used as an efficient method for developing new engineered insecticidal protein.

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Section: Discussionsupporting

confidence: 93%

Engineered chimeric insecticidal crystalline protein improves resistance to lepidopteran insects in rice (Oryza sativa L.) and maize (Zea mays L.)

Liu

Han

Yang

et al. 2022

Sci Rep

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“…The results in this study showed no significant differences on the composition, species richness ( S ), Shannon–Wiener diversity index ( H′ ), evenness index ( J ), Simpson’s dominant concentration ( C ), community similarity and ecosystem functioning composition of the NTAs community between transgenic insect-resistant maize (SK12-5 transgenic Cry1Ab/2Aj ) and the non-GE maize. These results are consistent with previous studies showing that Bt maize producing Cry1Ab [ 18 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ], Cry1Ab and Cry2Ab [ 45 ], Cry1A.105 and Cry2Ab [ 46 , 47 ], Cry1Ac [ 48 , 49 , 50 ], Cry1A.105 and Cry2Ab and Cry3Bb [ 51 ], Cry1Ab and Cry1Ac [ 52 ], Cry1Ab and VIP3A [ 53 ], Cry1F [ 54 , 55 , 56 ], Cry1Ah [ 57 ], Cry1Ie [ 58 , 59 , 60 ], Cry34Ab1 and Cry35Ab1 [ 55 , 61 ], Cry34Ab1 and Cry35Ab1 and Cry1F [ 55 , 61 ], Cry3Bb [ 62 , 63 , 64 , 65 ] and VIP 3A [ 66 ] insecticidal proteins did not affect populations of NTAs in the field. Similarly, there were no significant effects detected in the majority of such filed experiments with Bt cotton and Bt rice [ 37 , 67 , 68 , 69 ].…”

Section: Discussionsupporting

confidence: 93%

Impact of Transgenic Cry1Ab/2Aj Maize on Abundance of Non-Target Arthropods in the Field

Yang

Chen

Xue

et al. 2022

Plants

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Transgenic Bacillus thuringiensis (Bt) maize has broad prospects for application in China. Before commercialization, it is necessary to assess possible ecological impacts, including impacts on non-target arthropods (NTAs) in the field. In the present study, transgenic Bt maize expressing cry1Ab/2Aj and its corresponding non-transformed near isoline were planted under the same environmental and agricultural conditions, and arthropods in the field were collected during the three main growth stages of maize. In a one year trial, the results showed the composition of NTA communities in the transgenic and control maize fields were similar. There were no significant differences for community-level parameters of species richness (S), Shannon–Wiener diversity index (H′), evenness index (J) and Simpson’s dominant concentration (C) between the two types of maize fields. Likewise, a Bray–Curtis dissimilarity and distance analysis showed that Cry1Ab/2Aj toxin exposure did not increase community dissimilarities between Bt and non-Bt maize plots and that the structure of the NTAs community was similar on the two maize varieties. Furthermore, planting of the transgenic cry1Ab/2Aj maize did not affect the density or composition of non-target decomposers, herbivores, predators, parasitoids and pollinator guilds. In summary, our results showed that planting of Bt maize producing Cry1Ab/Cry2Aj proteins do not adversely affect population dynamics and diversity of NTAs.

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“…were unchanged, and organ biopsy results showed no disease and toxic effects (Hajimohammadi et al, 2021;Song et al, 2015;Wang et al, 2013b). In addition, neither germ cells nor somatic cells had mutagenic activity or presented a dose-independent effect (Carrière et al, 2009;Xing et al, 2019).…”

Section: Introductionmentioning

confidence: 91%

Evaluation of the Potential Effect of Cry1Ab Expressing Straw on ICR Mice

Tang

et al. 2021

Preprint

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Cry1Ab toxin has been effectively integrated into crops such as rice and cotton for pest control, and the safety evaluation of transgenic rice has attracted widespread attention. Nevertheless, the effects of transgenic rice straw on animal model are still unclear. Hence, the present study conducted an integrated analysis to evaluate the unintended effects of transgenic rice straw expressing Cry1Ab protein on the Institute of Cancer Research (ICR) mice under 90-day treatment. The results indicated that Cry1Ab rice straw had no significant effects on the behavior and body weight of mice. In addition, physiological indicators, including hemogram, blood biochemistry, apoptosis rate, and calcium ion concentration of the blood lymphocytes, displayed no alterations under Cry1Ab protein stress. Similarly, Cry1Ab rice straw had no adverse effects on several antioxidase activities (i.e., catalase, superoxide dismutase, peroxidase, glutathione peroxidase, and acetylcholine esterase). Moreover, we recorded that Cry1Ab stress did not adversely impact the sperm quality and follicular development of male and female ICR mice. Collectively, this integrated analysis indicates that Cry1Ab rice straw has no adverse or toxic effects on ICR mice after 90-day treatment and provides multi-level perspectives to assess the safety of genetically modified crops on non-target mammals.

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The impact of Bt maize expressing the Cry1Ac protein on non-target arthropods

Cited by 11 publications

References 25 publications

Engineered chimeric insecticidal crystalline protein improves resistance to lepidopteran insects in rice (Oryza sativa L.) and maize (Zea mays L.)

Engineered chimeric insecticidal crystalline protein improves resistance to lepidopteran insects in rice (Oryza sativa L.) and maize (Zea mays L.)

Impact of Transgenic Cry1Ab/2Aj Maize on Abundance of Non-Target Arthropods in the Field

Evaluation of the Potential Effect of Cry1Ab Expressing Straw on ICR Mice

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