The impact of cell structure, metabolism and group behavior for the survival of bacteria under stress conditions

Zhang, Xinyi; Li, Zhendong; Pang, Shengmei; Jiang, Boyu; Yang, Yang; Duan, Qiangde; Zhu, Guoqiang

doi:10.1007/s00203-020-02050-3

Cited by 12 publications

(2 citation statements)

References 119 publications

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“…Bacteria respond to stresses by activating various regulatory mechanisms including activities involved in metabolisms, cell envelope and gene expression, giving them the potentiality to adapt to extreme environmental conditions ( Figure 5 ) [108] . Changes in gene expression establish the principal component of the bacterial response [109] and can alter the biosynthesis of EPSs under stress conditions [110] .…”

Section: Changes the Expression Of Genes Involved In Eps Production Umentioning

confidence: 99%

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Nguyen¹,

Nguyen²,

Bui³

et al. 2020

AIMS Microbiology

134

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Exopolysaccharides (EPSs) are biological polymers secreted by microorganisms including Lactic acid bacteria (LAB) to cope with harsh environmental conditions. EPSs are one of the main components involved in the formation of extracellular biofilm matrix to protect microorganisms from adverse factors such as temperature, pH, antibiotics, host immune defenses, etc.. In this review, we discuss EPS biosynthesis; the role of EPSs in LAB stress tolerance; the impact of environmental stresses on EPS production and on the expression of genes involved in EPS synthesis. The evaluation results indicated that environmental stresses can alter EPS biosynthesis in LAB. For further studies, environmental stresses may be used to generate a new EPS type with high biological activity for industrial applications.

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Section: Changes the Expression Of Genes Involved In Eps Production Umentioning

confidence: 99%

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Nguyen¹,

Nguyen²,

Bui³

et al. 2020

AIMS Microbiology

134

View full text Add to dashboard Cite

show abstract

“…When microorganisms encounter changes in environmental conditions or stressors, their metabolic state undergoes alterations [21]. This can occur through adaptations or changes in gene expression and enzyme activity in response to stressful conditions [22,23]. Studies have shown that appropriately increasing the temperature can enhance the influx of precursor substances into the synthesis pathway of 2-PE in plants such as Camellia sinensis, tomato, and petunia, thereby increasing the overall synthesis of 2-PE [24].…”

Section: Introductionmentioning

confidence: 99%

Effects of Environmental Stresses on Synthesis of 2-Phenylethanol and IAA by Enterobacter sp. CGMCC 5087

Li,

Fang,

Zhang

et al. 2024

Microorganisms

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2-Phenylethanol (2-PE) and indole-3-acetic acid (IAA) are important secondary metabolites produced by microorganisms, and their production are closely linked to the growth state of microorganisms and environmental factors. Enterobacter CGMCC 5087 can produce both 2-PE and IAA depending on α-ketoacid decarboxylase KDC4427. This study aimed to investigate the effects of different environment factors including osmotic pressure, temperature, and pH on the synthesis of 2-PE and IAA in Enterobacter sp. CGMCC 5087. The bacteria exhibited an enhanced capacity for 2-PE synthesis while not affecting IAA synthesis under 5% NaCl and pH 4.5 stress conditions. In an environment with pH 9.5, the synthesis capacity of 2-PE remained unchanged while the synthesis capacity of IAA decreased. The synthesis ability of 2-PE was enhanced with an increase in temperature within the range of 25 °C to 37 °C, while the synthesis capacity of IAA was not affected significantly. Additionally, the expression of KDC4427 varied under stress conditions. Under 5% NaCl stress and decreased temperature, expression of the KDC4427 gene was increased. However, altering pH did not result in significant differences in gene expression levels, while elevated temperature caused a decrease in gene expression. Furthermore, molecular docking and molecular dynamics simulations suggested that these conditions may induce fluctuation in the geometry shape of binding cavity, binding energy, and especially the dαC-C- value, which played key roles in affecting the enzyme activity. These results provide insights and strategies for the synthesis of metabolic products 2-PE and IAA in bacterial fermentation, even under unfavorable conditions.

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Ultra‐Photostable Bacterial‐Seeking Near‐Infrared CPDs for Simultaneous NIR‐II Bioimaging and Antibacterial Therapy

Duan,

Li,

Liu

et al. 2024

Adv Healthcare Materials

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Bacterial infections can pose significant health risks as they have the potential to cause a range of illnesses. These infections can spread rapidly and lead to complications if not promptly diagnosed and treated. Therefore, it is of great significance to develop a probe to selectively target and image pathogenic bacteria while simultaneously killing them, as there are currently no effective clinical solutions available. This study presents a novel approach using near‐infrared carbonized polymer dots (NIR‐CPDs) for simultaneous in vivo imaging and treatment of bacterial infections. The core–shell structure of the NIR‐CPDs facilitates their incorporation into bacterial cell membranes, leading to an increase in fluorescence brightness and photostability. Significantly, the NIR‐CPDs exhibit selective bacterial‐targeting properties, specifically identifying Staphylococcus aureus (S. aureus) while sparing Escherichia coli (E. coli). Moreover, under 808 nm laser irradiation, the NIR‐CPDs exhibit potent photodynamic effects by generating reactive oxygen species that target and damage bacterial membranes. In vivo experiments on infected mouse models demonstrate not only precise imaging capabilities but also significant therapeutic efficacy, with marked improvements in wound healing. The study provides the dual‐functional potential of NIR‐CPDs as a highly effective tool for the advancement of medical diagnostics and therapeutics in the fight against bacterial infections.

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The impact of cell structure, metabolism and group behavior for the survival of bacteria under stress conditions

Cited by 12 publications

References 119 publications

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications

Effects of Environmental Stresses on Synthesis of 2-Phenylethanol and IAA by Enterobacter sp. CGMCC 5087

Ultra‐Photostable Bacterial‐Seeking Near‐Infrared CPDs for Simultaneous NIR‐II Bioimaging and Antibacterial Therapy

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