The purpose of this study to identify the impact of E-learning on the feeling of job alienation among faculty members in public and private Jordanian universities. The study community consisted of faculty members in all Jordanian university, where 498 questionnaires were analyzed. The descriptive-analytical approach was applied and the results were analyzed by statistical methods, the results of the study showed that there is a negative and statistically significant effect of E-learning on the extent to which faculty members feel job alienation, and that the rate of applying E-learning was at a medium degree, and the degree of faculty members’ feeling of job alienation was also at a medium degree. The study recommended not relying on E-learning completely, except in emergency cases, and limiting E-learning to some subjects, as the results showed that students' educational attainment was low, and the study recommended paying attention to the infrastructure of E-learning. The study dealt with a topic that the previous studies did not clearly address, and there is a paucity of studies that dealt with the subject of the study. which is the effect of e-learning on the feeling of job alienation among faculty members. These results may direct some researchers to conduct further studies on the impact of e-learning on the desire and effectiveness of faculty members in teaching.