In this groundbreaking experimental study, examines the impact of copper oxide (CuO) and Titanium oxide (TiO2) nanoparticles on both emissions and performance of a 4.1.5 kW diesel engine fueled by waste cooking oil biodiesel. B20 test fuel blends fortified with 50 ppm concentrations of CuO and TiO2 nanoparticles, subjected to rigorous testing across diverse engine loads (20%, 40%, 60%, and 80%) while maintaining a constant speed of 1,500 rpm. Through meticulous evaluation of key performance metrics such as brake thermal efficiency (BTE) and brake-specific fuel consumption (BSFC), coupled with the analysis of emissions including CO, CO2, HC, and NOx, we observed remarkable enhancements. Notably, the introduction of 50 ppm copper oxide and titanium oxide nanoparticles into biodiesel blends resulted in a notable boost in brake power (BP) and BTE, showcasing a remarkable surge from 12.39% to 22.14% in BP and 4.92% to 15% in BTE, underscoring their profound impact of particular significance, B2050CuO exhibited superior combustion characteristics compared to titanium nanoparticles, leading to elevated flame temperature and reduced ignition delay, thereby translating into improved engine performance, enhanced braking power output, and overall superior brake thermal efficiency. Furthermore, CuO and TiO2 nanoparticle blends demonstrated a significant reduction in CO emissions at full load with a 50 ppm dosage, achieving reductions of 6.32%, 8.57%, and 6.25%, respectively. Moreover, for B20 and B2050 TiO2 blends, HC emissions saw a notable drop by 9.14%, 6.57%, and 2.817%, promoting efficient combustion through enhanced fuel molecule interaction. Comparatively, the inclusion of B20, B2050TiO2, and B2050CuO fuel blends led to substantial reductions in CO2 levels by 14.4%, 4.8%, and 3.75%, along with lowered NOx emissions by 5.2%, 3.125%, and 2%, highlighting their potential in mitigating environmental impact."