The impact of diet on the composition and relative abundance of rumen microbes in goat

Abstract

ObjectiveThis experiment was conducted to explore the impact of diet on the ruminal microbial community in goats.MethodsTwelve goats were divided into two groups and fed complete feed (CF) or all forage (AF) diet. The total microbial DNAs in the rumen liquid were extracted. The V4 region of microbial 16S rRNA genes was amplified and sequenced using high-throughput. Information of sequences was mainly analyzed by QIIME 1.8.0.ResultsThe results showed that Bacteroidetes and Firmicutes were the most predominant microbial phyla in the rumen of all goats. At genus level, the abundance of fiber-digesting bacteria such as Ruminococcus and Lachnospiracea incertae sedis was significantly higher in AF than that in CF, while the levels of fat-degrading bacterium Anaerovibrio and protein-degrading bacterium Pseudomonas were opposite. The core shared genera, Prevotella and Butyrivibrio were widespread in the rumen of goats and no significant difference was observed in relative abundance between groups.ConclusionWe concluded that the richness of fiber-, protein-, and fat-digesting bacteria was affected by diet and tended to increase with the rise of their corresponding substrate contents in the ration; some bacteria shared by all goats maintained stable despite the difference in the ration, and they might be essential in maintaining the normal function of rumen.

Rumen bacterial diversity of Tibetan sheep (Ovis aries) associated with different forage types on the Qinghai-Tibetan Plateau

“…Firmicutes and Bacteroidetes, it still plays an important role in the formation and fermentation of biofilms and the digestion of soluble carbohydrates (Pitta et al 2016). In addition, it was reported that Proteobacteria had the highest abundance in the rumen of goat fed with complete feed (higher protein content while lower neutral detergent fiber and acid detergent fiber contents) than fed with all forage diet (Liu et al 2017). In the existing research, the soluble carbohydrates and protein contents were higher while neutral detergent fiber and acid detergent fiber contents were substantially lower in oat hay-diet than that in native pasture-diet.…”

Peer Review #1 of "Comparative analysis of the metabolically active microbial communities in the rumen of dromedary camels under different feeding systems using total rRNA sequencing (v0.1)"

“…The members of Proteobacteria were lower in G2 and G3 camels that were fed on diet rich in fiber contents. These findings highlighted this phylum's function as a protein-degrading bacteria (Liu et al, 2017). The abundance of Treponema was higher in the solid fraction and in G3 camels (Figure 1a).…”

Comparative Analysis of Soil Microbiome Profiles in the Companion Planting of White Clover and Orchard Grass Using 16S rRNA Gene Sequencing Data

“…We identified two unique species from Tenericutes and Spirochaetae that contributed to distinguishing the OG group from the other two groups. Tenericutes has been identified in regions with various kinds of grass orchards worldwide (Liu et al, 2017;Deakin et al, 2018). Spirochaetae has also been identified in regions planted with grass orchards (Brown, 1943).…”