The impact of DNA damage induced by sperm cryopreservation in Xenopus

Morrow, Sean; Guille, Matt; Holt, William V.; Garner, T.L.

doi:10.1530/repabs.1.p365

Cited by 2 publications

(2 citation statements)

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“…As a reduction of the S‐S bonds can be elicited in vitro by exposing spermatozoa to DTT, and as a low number of S‐S bonds are associated with immature cells, the percentage of spermatozoa which undergo in vitro decondensation of nuclear chromatin upon DTT incubation has been correlated with the number of immature cells (Rodríguez et al, ). There are also extrinsic factors affecting chromatin condensation such as the freeze‐thaw process (Lusignan, Li, Herrero, Delbes, & Chan, ) due to some of the components of the extender that destabilizes high melting region of polypeptide‐bound DNA and the extent of higher ordered structure in chromatin (Morrow, ). On the other side, cryopreservation has been associated with increased generation of reactive oxygen species (ROS) which in turn attack sperm DNA producing nicks and gaps (Peris, Bilodeau, Dufour, & Bailey, ).…”

Section: Discussionmentioning

confidence: 99%

“…It has been observed that incubation of frozen-thawed sperm with SP (Dominguez et al, 2008;Maxwell & Johnson, 1999;Mortimer & Maxwell, 2004) or with an isolated SP protein fraction (Barrios, 2002;Bernardini et al, 2011;Ledesma et al, 2016) with the number of immature cells (Rodríguez et al, 1985). There are also extrinsic factors affecting chromatin condensation such as the freeze-thaw process (Lusignan, Li, Herrero, Delbes, & Chan, 2018) due to some of the components of the extender that destabilizes high melting region of polypeptide-bound DNA and the extent of higher ordered structure in chromatin (Morrow, 2015). On the other side, cryopreservation has been associated with increased generation of reactive oxygen species (ROS) which in turn attack sperm DNA producing nicks and gaps (Peris, Bilodeau, Dufour, & Bailey, 2007).…”

Section: Discussionmentioning

confidence: 99%

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Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Ramírez-Vasquez

Cesari

Greco

et al. 2019

Reprod Domestic Animals

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Seminal plasma (SP) proteins interact with sperm plasma membrane (PM) modulating its functionality. It has been shown that SP proteins can reverse the damage caused by freeze‐thaw; however in these studies, SP has been added to washed sperm (i.e., cells depleted from homologous SP and extender). The aim of the current study was to assess whether the egg yolk‐based extender (EY) modifies SP ability to ameliorate sperm parameters in frozen‐thawed ram spermatozoa. Ejaculates were diluted in EY or soybean lecithin‐based extender (SL) and evaluated before and after freezing to measure the cell damage according to the extender. Even when all classical parameters decreased after freezing, as expected (p < .05), there was no effect of the extender. SP treatment was applied after freeze‐thaw. Sperm were incubated with SP (20% v/v) in the presence of either EY or SL, and sperm parameters were assessed after thawing compared with the same treatments after Percoll sperm selection (washed). Treatments with 20% SP improved sperm total and progressive motility compared with controls regardless of washing and extender (p < .05); however, washed sperm showed higher percentage of total sperm motility compared with those unwashed (p < .05). Moreover, treatment with 20% SP showed significantly higher percentages of PM integrity, sperm with intact acrosomes, integrity of chromatin and non‐capacitated sperm in samples diluted with EY when washed before treatment compared with the other conditions (p < .05). It was concluded that the presence of the extenders and particularly egg yolk alters the SP capacity to reduce the cryodamage.

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Section: Discussionmentioning

confidence: 99%