<b><i>Objective:</i></b> The aim of the study was to compare the efficiency and clinical outcomes of Moses contact mode (MCM) and regular dusting mode (RDM) during flexible ureteroscopic lithotripsy (FURL) for treatment of renal calculus. <b><i>Methods:</i></b> This retrospective analysis examined 216 patients with renal calculus who underwent FURL with MCM or RDM between March 2015 and January 2020. Stone characteristics, including size, volume, and density, were collected. Laser parameters, including laser type, laser working time, laser pause time, and foot-pedal use, were automatically recorded by the lithotripter work panel. The percentages of laser working time and laser pause time, stone fragmentation efficiency (SFE; volume/laser working time), postoperative complications, including fever and acute renal failure (ARF), stone-free rate (SFR), and the need for auxiliary procedures were determined. <b><i>Results:</i></b> There were no significant differences in preoperative demographic and stone characteristics between the MCM group and the RDM group. The MCM group had a shorter laser working time (4.99 ± 1.06 vs. 5.94 ± 0.96 min, <i>p</i> < 0.001) and a greater SFE (137.86 [163.78–114.38] versus 114.94 [132.06–101.34] mm<sup>3</sup>/min, <i>p</i> < 0.001), which shortened the overall operative time (18.39 ± 5.13 vs. 21.17 ± 6.78 min, <i>p</i> = 0.001). There were no differences in postoperative complications, including fever and ARF, SFR (86.8 vs. 85.3%, <i>p</i> = 0.743), and auxiliary procedures between the 2 groups. <b><i>Conclusions:</i></b> Using Moses laser technology with FURL significantly reduced laser working time and increased SFE, which shortened overall operative time. Urologists should consider this new instrument for the clinical management of renal calculus.