The impact of expired commercial drugs on non-target marine species: A case study with the use of a battery of biomarkers in hemocytes of mussels

Politakis, Nektarios; Belavgeni, Alexia; Efthimiou, Ioanna; Charalampous, Nikolina; Kourkouta, Chara; Dailianis, Stefanos

doi:10.1016/j.ecoenv.2017.10.021

Cited by 16 publications

(6 citation statements)

References 80 publications

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“…It combines an accurate measure of microplastic levels in target organisms, the concentrations of plastic additives and other persistent organic pollutants (POPs) in tissues and the corresponding toxicological effects. According to this new concept, mussels correspond to ideal biological models beacause they have been widely used as bioindicators of POPs in coastal environments (Aznar-Alemany et al, 2017;Martinović et al, 2016;Liu et al, 2014;Chiesa et al, 2018;Gagné et al, 2017;Chiu et al, 2018;Cunha et al, 2017;Politakis et al, 2018).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Using mussel as a global bioindicator of coastal microplastic pollution

Lusher

Rotchell

et al. 2019

Environmental Pollution

448

145

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Contents: 1. Introduction 2. Global field investigations on microplastic pollution in mussels 2.1 Selected species and geographic coverage 2.2 Characteristics of microplastic pollution 2.3 Methodological challenges 3. Laboratory exposures of microplastics in mussels 3.1 Uptake, accumulation and clearance of microplastics 3.2 Toxic effects of microplastics 3.3 Optimization of laboratory exposures 4. Scope of mussels as global bioindicators of microplastic 4.1 Advantages of utilizing mussel 4.2 Current regional and national proposals 4.3 Future developments

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Section: Accepted Manuscriptmentioning

confidence: 99%

Using mussel as a global bioindicator of coastal microplastic pollution

Lusher

Rotchell

et al. 2019

Environmental Pollution

448

145

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“…Reported rates range from 40% to 50% for overexpression or 25% to 40% for mutation, similar to other malignancies . Although CTC assays have individually proven successful in finding a tumor origin, further research is warranted to compare detection rates when used to evaluate the same patient samples .…”

Section: Discussionmentioning

confidence: 97%

Molecular Profiles of Brain and Pulmonary Metastatic Disease in Cancer of Unknown Primary

et al. 2020

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Cancers of unknown primary (CUP) are histologically confirmed malignancies but for which further investigation cannot identify a primary site. Improvements in histopathologic modalities for diagnosis have lessened the frequency of CUPs to 3%-5% of all malignancies compared with historical estimates of 5%-10%. Despite this, there is an ongoing debate as to whether CUPs are malignancies where the primary is not found or if they are otherwise a fully separate entity. Improvements in molecular analysis holds promise for improved identification and treatment of CUPs with mixed preliminary results. Here we present a woman with CUP and metastases in her brain and lung. We performed genomic profiling to compare the molecular makeup of each site in order to establish treatment targets. The Oncologist 2020;25:555-559 KEY POINTS • Cancer of unknown primary remains a diagnostic and therapeutic challenge. • Molecular analysis may provide improvements in diagnosis and novel treatment options. • Different sites of metastatic disease have subtle variations in molecular profile. • Sequencing of different sites may offer therapeutic options that are either already approved or available in clinical trial.

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“…The determination of stress indices in hemolymph of the species Mytilus galloprovincialis is widely used for assessing the type of pressure aquatic ecosystems are faced with, as well as the adverse effects of different types of pollutants (either alone or in mixtures) with great precision and reproducibility [ 39 , 41 , 76 , 77 ]. Since mussel hemocytes represent the main immune defensive cells in bivalves [ 78 ] the present study showed for the first time that PCMBs leaching into marine media (ASW) could disturb hemocyte lysosomal membrane integrity.…”

Section: Discussionmentioning

confidence: 99%

“…In any case, almost 10 mL of hemolymph were withdrawn from the posterior adductor muscle of 10 individuals (N = 10; 1 mL mussel −1 ; control- and CPBM-treated), using a sterile 1 mL syringe with an 18 G1/2′′ needle, containing equal volume of Alseve buffer [ 41 ]. A small portion of hemolymph from each mussel per group was used for the determination of lysosomal membrane stability in mussel hemocytes (using the neutral red retention time/NRRT) assay) and the cytogenetic analysis of DNA integrity (using the micronuclei/MN assay) (N = 10 in any case).…”

Section: Methodsmentioning

confidence: 99%

The Potential Risk of Electronic Waste Disposal into Aquatic Media: The Case of Personal Computer Motherboards

Kalamaras

Kloukinioti

Αντωνοπούλου

et al. 2021

Toxics

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Considering that electronic wastes (e-wastes) have been recently recognized as a potent environmental and human threat, the present study aimed to assess the potential risk of personal computer motherboards (PCMBs) leaching into aquatic media, following a real-life scenario. Specifically, PCMBs were submerged for 30 days in both distilled water (DW) and artificial seawater (ASW). Afterwards, PCMBs leachates were chemically characterized (i.e., total organic carbon, ions, and trace elements) and finally used (a) for culturing freshwater (Chlorococcum sp. and Scenedesmus rubescens) and saltwater (Dunaliella tertiolecta and Tisochrysis lutea) microalgae for 10 days (240 h), (b) as the exposure medium for mussel Mytilus galloprovincialis (96 h exposure), and (c) for performing the Cytokinesis Block Micronucleus (CBMN) assay in human lymphocytes cultures. According to the results, PCMBs could mediate both fresh- and marine algae growth rates over time, thus enhancing the cytotoxic, oxidative, and genotoxic effects in the hemocytes of mussels (in terms of lysosomal membrane impairment, lipid peroxidation, and NO content and micronuclei formation, respectively), as well as human lymphocytes (in terms of MN formation and CBPI values, respectively). The current findings clearly revealed that PCMBs leaching into the aquatic media could pose detrimental effects on both aquatic organisms and human cells.

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The impact of expired commercial drugs on non-target marine species: A case study with the use of a battery of biomarkers in hemocytes of mussels

Cited by 16 publications

References 80 publications

Using mussel as a global bioindicator of coastal microplastic pollution

Using mussel as a global bioindicator of coastal microplastic pollution

Molecular Profiles of Brain and Pulmonary Metastatic Disease in Cancer of Unknown Primary

The Potential Risk of Electronic Waste Disposal into Aquatic Media: The Case of Personal Computer Motherboards

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