The impact of foot shock-induced stress on pain-related behavior associated with burn injury

Wu, Pau Yen; Menta, Blaise; Visk, Alexander; Ryals, Janelle M.; Christianson, Julie A.; Wright, Douglas E.; Chadwick, Andrea

doi:10.1016/j.burns.2021.04.010

Cited by 2 publications

(1 citation statement)

References 60 publications

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“…The 50% withdrawal threshold was calculated for each mouse, and group means were determined as previously described. 8 , 66 Cold allodynia was assessed as previously described. 6 Briefly, mice were acclimated on 1/4″ Pyrex borosilicate glass in clear plastic chambers (11 × 5 × 3.5 cm).…”

Section: Methodsmentioning

confidence: 99%

Vagus nerve stimulation rescues persistent pain following orthopedic surgery in adult mice

Wu,

Caceres,

Chen

et al. 2024

Pain

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Postoperative pain is a major clinical problem imposing a significant burden on patients and society. In a survey 2 years after orthopedic surgery, 57% of patients reported persisting postoperative pain. However, only limited progress has been made in the development of safe and effective therapies to prevent the onset and chronification of pain after orthopedic surgery. We established a tibial fracture mouse model that recapitulates clinically relevant orthopedic trauma surgery, which causes changes in neuropeptide levels in dorsal root ganglia and sustained neuroinflammation in the spinal cord. Here, we monitored extended pain behavior in this model, observing chronic bilateral hindpaw mechanical allodynia in both male and female C57BL/6J mice that persisted for >3 months after surgery. We also tested the analgesic effects of a novel, minimally invasive, bioelectronic approach to percutaneously stimulate the vagus nerve (termed percutaneous vagus nerve stimulation [pVNS]). Weekly pVNS treatment for 30 minutes at 10 Hz for 3 weeks after the surgery strongly reduced pain behaviors compared with untreated controls. Percutaneous vagus nerve stimulation also improved locomotor coordination and accelerated bone healing. In the dorsal root ganglia, vagal stimulation inhibited the activation of glial fibrillary acidic protein-positive satellite cells but without affecting microglial activation. Overall, these data provide novel evidence supportive of the use of pVNS to prevent postoperative pain and inform translational studies to test antinociceptive effects of bioelectronic medicine in the clinic.

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Section: Methodsmentioning

confidence: 99%

Vagus nerve stimulation rescues persistent pain following orthopedic surgery in adult mice

Wu,

Caceres,

Chen

et al. 2024

Pain

Self Cite

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Serotonin mediates stress-like effects on responses to non-nociceptive stimuli in the medicinal leech Hirudo verbana

Mack

Yevugah

Renner

et al. 2022

Journal of Experimental Biology

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Noxious stimuli can elicit stress in animals that produce a variety of adaptations including changes in responses to nociceptive and non-nociceptive sensory input. One example is stress-induced analgesia that may be mediated, in part, by the endocannabinoid system. However, endocannabinoids can also have pro-nociceptive effects. In this study, the effects of electroshock, one experimental approach for producing acute stress, were examined on responses to non-nociceptive mechanical stimuli and nociceptive thermal stimuli in the medicinal leech (Hirudo verbana). The electroshock stimuli did not alter Hirudo's responses to nociceptive stimuli, but did cause sensitization to non-nociceptive stimuli, characterized by a reduction in response threshold. These experiments were repeated with drugs that either blocked synthesis of the endocannabinoid transmitter 2-arachidonoylglycerol (2-AG) or transient receptor potential vanilloid (TRPV) channel which is known to act as an endocannabinoid receptor. Surprisingly, neither treatment had any effect on responses following electroshock. However, the electroshock stimuli did reliably increase serotonin (5-hydroxytryptamine or 5HT) levels in the Hirudo CNS. Injection of 5HT mimicked the effects of the electroshocks, sensitizing responses to non-nociceptive stimuli and having no effect on responses to nociceptive stimuli. Injections of the 5HT receptor antagonist methysergide reduced the sensitization effect to non-nociceptive stimuli after electroshocks treatment. These results indicate that electroshocks enhance response to non-nociceptive stimuli but does not alter responses to nociceptive stimuli. Furthermore, while 5HT appears to play a critical role in this shock-induced sensitizing effect, the endocannabinoid system seems to have no effect.

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The impact of foot shock-induced stress on pain-related behavior associated with burn injury

Cited by 2 publications

References 60 publications

Vagus nerve stimulation rescues persistent pain following orthopedic surgery in adult mice

Vagus nerve stimulation rescues persistent pain following orthopedic surgery in adult mice

Serotonin mediates stress-like effects on responses to non-nociceptive stimuli in the medicinal leech Hirudo verbana

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