The increasing frequency of bushfires rapidly alters forest ecosystems by transforming their vegetation structure and composition. This phenomenon poses a significant threat to biodiversity, as it hinders the forest's ability to regenerate and enhances its variability. Despite the urgent need to understand these impacts, comprehensive studies documenting the effects of bushfires on vegetation, particularly in the Opro River Forest Reserve, remain scarce. This study aims to assess the influence of bushfires on the vegetation structure and composition within the Opro River Forest Reserve. The research was conducted at two distinct sites: burnt and unburnt sites of the forest reserve. Sampling involved the establishment of twenty 25 m × 25 m plots, with ten plots designated for each site. Trees with a diameter at breast height (dbh) of 10 cm or greater were recorded, alongside nested plots of 5 m × 5 m for shrubs (dbh < 10 cm and height > 1.5 m). A total of 75 plant species across 31 families were identified in both sites. In the unburnt forest site, trees were the dominant woody plants, with Broussonetia papyrifera being the most prevalent species. Conversely, the burnt site of the forest was primarily dominated by shrubs, particularly Chromolaena odorata. The Shannon diversity index was higher in the unburnt site (0.36) compared to the burnt site (0.34), although species evenness and the total number of individuals were greater in the burnt area. Notably, tree diversity significantly declined in the burnt site relative to the unburnt site.