The Impact of Fortification of Mulberry Leaves with the Yeast Saccharomyces cerevisiae and the Blue Green Algae Spirulina platensis on some Quantitative Parameters of Silkworm Bombyx mori (L.)

Abstract

The present investigation was conducted to study the effect of fortification of mulberry leaves with different concentrations of baker's yeast Saccharomyces cerevisiae extract and blue-green algae Spirulina platensis extract on some quantitative parameters of Bombyx mori larvae and cocoons. Statistical analysis of the obtained data revealed that all larval treatments showed a significant increase in the larval and cocoon weights, especially at concentrations 0.5 % of yeast and 0.05% of spirulina. Feeding 5 th larval instar with mulberry leaves at concentrations 0.5 % yeast and 0.05% spirulina, lead to increase the larval weight by about 55 % and 30%, respectively, when compared with the control. The same trend was observed in pupal weight, cocoon weight and cocoon shell weight. Larval treatments with yeast extract at concentration 0.5% increased the weights of pupa (0.850 gm), cocoon (0.908 gm) and cocoon shell (0.216 gm) by about 30%, 16% and 27% as compared with controls which were 0.561gm, 0.781gm and 0.170 gm, respectively. On the other hand, all larval trials with spirulina extracts remained at the second grade of implementation on the pupal weight, cocoon weight and shell weight. However, feeding B. mori larvae with yeast extract significantly increased the silk ratio and the total haemolymph protein comparing with the spirulina treatments or control ones.

Effects of Spirulina platensis mediated TIO2NPS on biochemical analysis (Bombyx mori. L)

“…The total haemolymph protein, haemolymph carbohydrates, haemolymph lipids were significantly increased due to the feeding the larvae with S. platensis mediated TiO 2 NPs at concentration of 50 ppm treated mulberry leaves (8.62 mg/ml and 8.63 mg/ml, 14.96 mg/ml and 14.97 mg/ml, 19.99 ml/g and 20.00 mg/g) as compared to control (7.68 mg/ml and 7.69 mg/ml, 13.97 mg/ml and 13.99 mg/ml, 19.05 ml/g and 19.06 mg/g) (Figure 1). This result corroborates with the findings of Soliman et al (2021) who observed that higher amount of total haemolymph protein at 0.05 percentage of Spirulina treated mulberry leaves. Similar, findings have been reported by Rani et al (2011) who recorded that supplementation of mulberry leaves with probiotics like S. cerevisiae and Spirulina increased the haemolymph protein and amino acids.…”

Adaptability and Comparative analysis of Economic and Biological Traits of various Silkworm Races (Bombyx mori L.)

“…A small cut was made with a knife on each cocoon at its one end and the pupa was removed to calculate the shell weight of each cocoon. The shell ratio of cocoon was calculated by the given formula: (Soliman, 2021) The protein (Sericin and Fibroin) contents were estimated by degumming method (Mumtaz et al, 2022). After degumming, the fibroin content was equal to dry weight of fibroin while given formula was used to evaluate sericin:…”

Effect of amino acid fortified mulberry leaves on economic and biological traits of Bombyx mori L.

“…These supplementations not only provide the necessary nourishment for improved growth and development but also improve economic characteristics [ 23 ]. Recently, amino acid fortified mulberry leaves has been introduced in Sericulture industry to improve the economic and biological traits of silkworm (larval growth, cocoon shell size and weight of silkworm) and the quality of silk produced [ [ [25] , [26] , [27] ]]. They can regulate and adjust the physiological function and metabolism of silkworms by joining active substances such as hormones and enzymes.…”