The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes

Andrade, Catherine; Amenabar, Felipe Ugarte; Inostroza, Carolina; Díaz-Calderón, Paulo; Messina, David Rosenberg; Carrasco, Nelson Pinto; Quirynen, Marc

doi:10.1016/j.jobcr.2022.08.020

Cited by 6 publications

(11 citation statements)

References 50 publications

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“…Even in diabetic patients, the same differences in fibrinogen levels were observed between the two genders, again with increased values in women. Fibrinogen levels are directly associated with fibrin clot properties (structure, time to form and resistance to fibrinolysis), and therefore they may be a key parameter in determining the characteristics of membranes derived from self compressed platelet-rich fibrin (PRF) clots (Aldana et al, 2022) 12 . The ROC curve analysis in the work of Li et al (2016) suggested that the optimal fibrinogen cut-off point for complete amputation among the 152 patients they examined with DFU was 513 mg/dL, with sensitivity, specificity, PPV (Positive Predictive Value) and NPV (Negative Predictive Value) of 80.9%, 82.6%, 78.6% and 89.0%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Monocytes and Fibrinogen as Biomarkers in Type 2 Diabetes Mellitus

Crisci,

Flagiello,

Lepore

et al. 2024

IJMSCRS

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Background: In the diagnosis of diabetic foot, in addition to the inflammatory biomarkers that have been widely interested and used, for example procalcitonin, C-reactive protein (CRP), ESR, leukocyte count, neutrophil count, Fibrinogen and Monocytes are considered as biomarkers in this study. possible. Methods: A prospective study was designed to examine the utility of fibrinogen and monocytes in estimating disease severity in patients with DFU (Diabetic Foot Ulcer). The severity of DFU was assessed using the Wagner criteria distinguishing between patients with diabetic foot without ulcer (WDFU) and with non-infected diabetic foot ulcer (NIDFU) or with infected ulcer (IDFU). In this study the AA also wanted to correlate HbA1c to the concentration of Fibrinogen and the appearance of DFU, as well as the level of lymphocytes and monocyte precursors of macrophages in the evolution of ulcerated and non-ulcerated diabetic feet. Results: Mean blood fibrinogen values were significantly higher in patients with DFU grade ≧2 compared to those with DFU grade ≤1 (424.4±138.8 mg/dL versus 395.3±130.0 mg/dL; p=0.091). Fibrinogen values were correlated with CRP levels, neutrophils, ESR and leukocyte count. Monocytes presented a significant difference between non-diabetic patients with ulcer and without ulcer (0.41 vs 0.29 k/L; p=0.000) and between the diabetic without ulcer (WDFU) and non-diabetic without ulcer (NDWU) groups (0.39 vs 0.29 k/L; p=0.000). The Procalcitonin (PCT) value was <0.5 ng/dl, therefore it had no diagnostic significance. Only 1% of the values found were higher than 0.5 with an average of 1.04 ng/dl (range: 0.52-2.5). Conclusions: Neither monocytes nor HbA1c can be considered biomarkers for the risk of ulcer formation in the diabetic foot, but only as biomarkers of type 2 Diabetes Mellitus. Differently, fibrinogenemia, its pre/post intervention ratio, the  angle and the k value of thromboelastography (TEG), have a clinical significance on the risk of onset and development of ulcerated diabetic foot. The cut-off for ulcer formation for both the pre/post intervention ratio of fibrinogenemia and monocythemia is 1.10, with a sensitivity of 84.1% and a specificity of 24.5% for fibrinogen and 93.8% and 14.8% for monocytes.

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Section: Resultsmentioning

confidence: 99%

Monocytes and Fibrinogen as Biomarkers in Type 2 Diabetes Mellitus

Crisci,

Flagiello,

Lepore

et al. 2024

IJMSCRS

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“…We have implemented a construct of fibrinagarose that was easy to manipulate, allowed the inclusion of stem cells derived from dental tissues, and incorporated PRP factors that stimulate cell proliferation, 24 hence creating a suitable environment for the potential use in tissue reparation or regeneration.…”

Section: Discussionmentioning

confidence: 99%

Dentin slice model of dental stem cells in a fibrin-agarose construct for dental pulp regeneration

Inostroza¹,

Brizuela²,

Hernández³

et al. 2022

J Oral Res

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Objectives: To implement a dentin slice model of mesenchymal stem cells derived from dental tissues in a fibrin-agarose construct for dental pulp regeneration. Material and Methods: MSCs derived from different oral cavity tissues were combined with a fibrin-agarose construct at standard culture conditions. Cell viability and proliferation tests were assayed using a fluorescent cell dye Calcein/Am and WST-1 kit. The proliferation assay was evaluated at 24, 48, 72, and 96 hours. Also, we assessed the dental pulp stem cells (DPSCs) cell morphology inside the construct with histological stains such as Hematoxylin and Eosin, Masson's trichrome, and Periodic acid–Schiff. In addition, we elaborated a tooth dentin slice model using a culture of DPSC in the fibrin–agarose constructs co-adhered to dentin walls. Results: The fibrin-agarose construct was a biocompatible material for MSCs derived from dental tissues. It provided good conditions for MSCs' viability and proliferation. DPSCs proliferated better than the other MSCs, but the data did not show significant differences. The morphology of DPSCs inside the construct was like free cells. The dentin slice model was suitable for DPSCs in the fibrin-agarose construct. Conclusion: Our findings support the dentin slice model for future biological use of fibrin-agarose matrix in combination with DPSCs and their potential use in dental regeneration. The multipotency, high proliferation rates, and easy obtaining of the DPSCs make them an attractive source of MSCs for tissue regeneration.

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“…The dehydration of the fibrin clots released a GF-rich exudate that could be collected with a syringe and used (Figure 2C). The PRF membranes were composed of three zones: the "face" (or red zone), representing the area that was in contact with the RBCs after centrifugation, the "tail" located in the upper zone, and an intermediate zone called the "body" [19,20] (Figures 3 and 4). Finally, the fibrin clot was dehydrated between two flat surfaces to obtain membranes (Figure 2A) or in a cylinder with a piston on top to obtain plugs (Figure 2B).…”

Section: Methodsmentioning

confidence: 99%

Understanding Solid-Based Platelet-Rich Fibrin Matrices in Oral and Maxillofacial Surgery: An Integrative Review of the Critical Protocol Factors and Their Influence on the Final Product

Salgado-Peralvo,

Kewalramani,

Pérez-Jardón

et al. 2023

Medicina

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Platelet-rich fibrin (PRF) is a second-generation platelet concentrate whose use in clinical practice has been widely disseminated. This has led to the development of several commercial protocols, creating great confusion as to the terminology and implications of each of them. This integrative review aims to identify the critical factors of each of the phases of the solid-based PRF matrix protocol and their possible influence on their macro- and microscopic characteristics. An electronic search of the MEDLINE database (via PubMed), Web of Science, Scopus, LILACS, and OpenGrey was carried out. The search was temporarily restricted from 2001 to 2022. After searching, 43 studies were included that met the established criteria. There were numerous factors to consider in the PRF protocol, such as the material of the blood collection tubes, the duration of phlebotomy, the parameters related to blood centrifugation, the time from centrifugation to dehydration of the fibrin clots and their dehydration into membranes, as well as the time to clinical use. These factors influenced the macro- and microscopic characteristics of the PRF and its physical properties, so knowledge of these factors allows for the production of optimised PRF by combining the protocols and materials.

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The impact of gender and peripheral blood parameters on the characteristics of L-PRF membranes

Cited by 6 publications

References 50 publications

Monocytes and Fibrinogen as Biomarkers in Type 2 Diabetes Mellitus

Monocytes and Fibrinogen as Biomarkers in Type 2 Diabetes Mellitus

Dentin slice model of dental stem cells in a fibrin-agarose construct for dental pulp regeneration

Understanding Solid-Based Platelet-Rich Fibrin Matrices in Oral and Maxillofacial Surgery: An Integrative Review of the Critical Protocol Factors and Their Influence on the Final Product

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