The Impact ofLactobacillus plantarumon the Gut Microbiota of Mice with DSS-Induced Colitis

Abstract

The pathogenesis of inflammatory bowel disease (IBD) is due in part to a loss of equilibrium among the gut microbiota, epithelial cells, and resident immune cells. The gut microbiota contains a large proportion of probiotic commensalLactobacillusspecies; some natural microbiota and probiotics confer protection against IBD. In this study, mice with colitis triggered by dextran sodium sulphate (DSS) were givenLactobacillus plantarumorally. We assessed the damage caused by DSS and the therapeutic activity ofL. plantarum. The colitis triggered by DSS was less severe in the mice that received theL. plantarumtreatment, which also diversified the microbe species in the colon, enhanced the ratio of Firmicutes to Bacteroidetes, and diminished the relative abundance ofLactobacillus. The taxonomic units of greatest diversity in the DSS andL. plantarumgroups were identified using a linear discriminant and effect size (LEfSe) analysis.Aliihoefleawas established to be the genus of bacteria that was affected in theL. plantarumgroup most extensively. In conclusion, gut health was promoted byL. plantarum, as it diversified the microbes in the colon and restricted the activity of pathogenic bacteria in the intestine. Moreover, according to the LEfSe analysis, the DSS group was impacted more significantly by gut microorganisms than theL. plantarumgroup, suggesting thatL. plantarumimproved the stability of the intestinal tract.

Traditional Chinese Medicine Formula Kang Shuai Lao Pian Improves Obesity, Gut Dysbiosis, and Fecal Metabolic Disorders in High-Fat Diet-Fed Mice

“…It seems that carnivorous individuals harbor abundance of Christensenellaceae_R-7_group, assigned to the Firmicutes phylum (Huang, 2018). Besides, destined as pathological Proteobacteria, the appearance of Aliihoeflea could be recognized as a sign reflecting gut disorder during the course of dextran sodium sulfate (DSS)-triggered colitis (Zhang et al, 2019a). In view of the above evidences, we believe that Intestinimonas, Oscillibacter, Christensenellaceae_R-7_group, and Aliihoeflea play crucial roles regarding the prevention effect of KSLP against HFD-induced obesity.…”

Lactobacillus paracasei-derived extracellular vesicles attenuate the intestinal inflammatory response by augmenting the endoplasmic reticulum stress pathway

“…The human microbiome has been extensively investigated for its role in the prevention and treatment of inflammatory bowel disease (IBD) 1,2 . Humans are populated with trillions of bacteria that are located primarily in the gastrointestinal tract and have vast, complex immunomodulatory effects on their host.…”

Ameliorative Effect of Heat-Killed Lactobacillus plantarum L.137 and/or Aloe vera against Colitis in Mice

“…Beneficial products resulted from cracking in the bacterial cell wall which lead to release of different cell components and cytoplasmic contents, including lipoteichoic acids, peptidoglycans, exopolysaccharides, and organelles as DNA and cell wall components [48], and can also inhibit pathogens [49]. The immunomodulatory activities of L. plantarum are conferred by suppressing pro-inflammatory cytokines and blocking different inflammatory signaling pathways, including toll-like receptor (TLR-4)-linked NF-κB and MAPK complexes [50,51]. Furthermore, L. plantarum possessed a powerful antioxidant function manifested by its ability to increase cellular antioxidants and suppress lipid peroxidation [52].…”

“…Although the mechanism underlying the suppression of TNF-α and IL-6 by Aloe is unclear, the role of the chromone moiety in inhibiting NF-κB activation elicited by TNF-α has been acknowledged [20,60]. In the same context, L. plantarum has been reported to attenuate inflammation by inhibiting NF-κB signaling [50,51]. Therefore, the protective activity of Aloe and HK L.137 is mediated, at least in part, via inhibiting NF-κB activation.…”