The impact of insecticide applications on the dynamics of resistance: The case of four Aedes aegypti populations from different Brazilian regions

Garcia, Gabriela A.; David, Mariana Rocha; Martins, Ademir Jesús; Maciel-de-Freitas, Rafael; Linss, Jutta; Araújo, Simone Costa; Lima, José Bento Pereira; Valle, Denise

doi:10.1371/journal.pntd.0006227

Cited by 74 publications

(78 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We also hypothesized that KDR‐type mutation is likely a prevalent mechanism leading to cross‐resistance between DDT and pyrethroids. This is because these compounds share the same mode of action and altered target site resistance was frequently reported in the region . Our results confirmed the expectation of widespread insecticide resistance in mosquito populations in Latin America and the Caribbean, particularly for the most commonly used insecticides.…”

Section: Discussionsupporting

confidence: 88%

“…The lack of effective vaccines or pharmaceutical treatments for many of these neglected diseases places mosquito vector control in the forefront of the preventive initiatives against these maladies . Vector control, however, is no easy task and is achieved mainly through the use and overuse of insecticides against mosquito larvae and/or adults favoring the frequent development of insecticide resistance in the targeted insect populations …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Patterns of insecticide resistance in Aedes aegypti: meta‐analyses of surveys in Latin America and the Caribbean

Guedes

Beins

Costa

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: The re-emergence of worldwide concern with arthropod-borne viruses (arboviruses) draws increasing attention to their mosquito vectors, particularly Aedes aegypti, whose control heavily rely on insecticide use. As a consequence, insecticide resistance is frequent, but the general patterns of occurrence, cross-resistance and prevailing mechanisms remain unrecognized in some areas such as the Neotropical region. Thus, we sought here to recognize the general trends and patterns of insecticide resistance in Latin America and the Caribbean. A systematic literature review (2008)(2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017)(2018) aimed the data-gathering for the region and meta-analyses to address the stated knowledge gap.RESULTS: A high incidence of insecticide resistance prevails in the mosquito populations of the region. Dichlorodiphenyltrichloroethane (DDT), temephos and deltamethrin were the main insecticides evaluated and the meta-analyses indicate a high frequency of DDT-resistant populations (86.7 ± 0.1%), followed by temephos (75.7 ± 0.1%) and deltamethrin (33.0 ± 0.1%). No evidence of cross-resistance was detected among these three insecticides, and the V1016I knockdown (KDR) site mutation does not explain the patterns of deltamethrin resistance in the region.CONCLUSION: Resistance to DDT, temephos and deltamethrin is serious and widespread, and there is no cross-resistance among them. Altered target site sensitivity is not the main pyrethroid resistance mechanism, which is likely due to a mix of mechanisms. Therefore, the replacement of deltamethrin and particularly temephos in the region by alternative insecticides is an important resistance management recommendation, but should be done with compounds out of the cross-resistance spectrum for these populations and insecticides. Nonetheless, the non-recognition of the prevalent resistance mechanisms in the region makes this suggestion more difficult to apply and invites more broad-scale studies of resistance mechanisms to fill this knowledge gap and improve the resistance management recommendations.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Patterns of insecticide resistance in Aedes aegypti: meta‐analyses of surveys in Latin America and the Caribbean

Guedes

Beins

Costa

et al. 2020

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…In Campo Grande, the highest RR values (above 50 for deltamethrin) revealed that the period with the highest incidence of dengue coincides with the detection of Ae. aegypti populations with high resistance 38 .…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Aedes aegypti populations to pyriproxyfen in the Federal District of Brazil

Carvalho

Germano

Braga

et al. 2020

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: In Brasilia, pyriproxyfen (PPF; 0.01 mg/L) has been used for the larval control of Aedes aegypti mosquitoes since 2016. Information on the susceptibility of Ae. aegypti to PPF, and the development of resistance in populations from the Federal District of Brazil (FD) is limited. It is essential to monitor the susceptibility of Ae. aegypti to insecticides in order to improve vector control strategies. This study aimed to evaluate the susceptibility of Ae. aegypti populations from five areas of Brasilia to PPF. Methods: We performed dose-response tests to estimate the emergence inhibition and resistance ratio of each field population, including the Rockefeller reference population. We also analyzed egg positivity, and the density and mortality of larvae and pupae. Results: Populations from Vila Planalto (RR 50 =1.7), Regiment Guards Cavalry (RR 50 =2.5), and Sub-secretary of Justice Complex (RR 50 =3.7) presented high susceptibility to PPF, while the RR values of populations from Lago Norte (RR 50 =7.7) and Varjão (RR 50 =5.9) were moderately high, suggesting the emergence of insipient resistance to PPF in Brasilia. At 30 ng/mL, the highest larvae mortality rate was 2.7% for the population from Lago Norte, while that of pupae was 92.1% for Varjão and Vila Planalto. Conclusions: The five populations of Ae. aegypti from the FD are susceptible to PPF and there is a need to monitor the susceptibility of Ae. aegypti in new areas of the FD.

show abstract

“…In America, kdr mutations have been reported in Ae. aegypti populations from Ecuador, (22), United States (23, 24), Colombia (25, 26), México (27–29), Brazil (30–35), Lesser Antilles (36–40), French Guiana (37), Venezuela (29, 41), Cuba (29, 39), Panamá (42), Puerto Rico (43). This is the first report of kdr mutations for the countries included in this study, except for Haiti (87).…”

Section: Discussionmentioning

confidence: 99%

“…Resistance to pyrethroids in mosquitoes have been widely associated with the F1534C (21, 23, 25, 27, 29, 30, 32, 39–41, 83, 88–101) and V1016I mutations (24, 25, 27, 29, 30, 32, 36, 38, 40, 41, 88, 93, 97, 100, 102, 103). The simultaneous presence of both mutations has been associated with enhanced tolerance to deltamethrin in the past (31).…”

Section: Discussionmentioning

confidence: 99%

The need to harmonize insecticide resistance testing: methodology, intensity concentrations and molecular mechanisms evaluated inAedes aegyptipopulations in Central America and Hispaniola

Ledoux

Gutiérrez

Lobo

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundThe Zika AIRS Project, a USAID-funded initiative worked across the Latin America and Caribbean regions from 2016 to 2019, as an emergency to contain the spread of the Zika virus. All entomological records in the target countries showed wide distribution and high abundance of Aedes aegypti populations, however the susceptibility profiles of these insects to insecticides commonly employed by vector control campaigns were in most cases incomplete or inexistent. In close collaboration with the Ministries of Health of individual countries, Zika-AIRS teams conducted insecticide susceptibility testing of an array of insecticides in A. aegypti populations of each country. Procedures applied met the standard international protocols instructed by the World Health Organization and Centers for Disease Control and Prevention.Methodology and main findingsThe insecticides tested were selected under categories such as pyrethroids, organophosphates and carbamate. Results showed A. aegypti populations displaying high and widely distributed resistance to all pyrethroids across countries, tolerance to organophosphates and full susceptibility to a carbamate. Key inconsistencies between testing methods are presented and discussed. Additionally, four kdr mutations were analyzed to detect molecular mechanisms of insecticide resistance. The screening for kdr mutations suggested the widespread nature of V1016I mutation, linked to pyrethroid resistance in A. aegypti populations distributed and sampled in the above mentioned regions.Conclusions and perspectivesThis multi-country study contributes with updated information to the public health decision-makers across Central America and the Caribbean. This study provided training and established technical networks for more effective and sustainable insecticide surveillance programs. Most but not all records of insecticide resistance in A. aegypti were consistent between methodologies, thus inconsistent issues are discussed here to call for further improvement in procedures and convey more practical guidelines for surveillance teams in countries where Aedes-borne diseases are endemic.Author summaryAt the forefront of the fight against arboviruses transmission is the insecticide-based vector control. All countries in the Latin American and Caribbean region invest valuable resources from their limited budget to acquire and implement insecticide-based tools, with non-existent or weak insecticide resistance monitoring programs. Hence, the USAID-funded Zika AIRS Project (ZAP) collaborated with the Ministries of Health of multiple countries to update the profile of susceptibility to insecticides in Aedes aegypti populations. We found widespread resistance to pyrethroid and organophosphate insecticides, which account to almost 100% of the products available to control adult mosquitoes. As we used both of World Health Organization and Centers for Disease Control and Prevention standard methods, we found many similarities and some inconsistencies in the susceptibility profiles obtained for the very same vector populations. Additionally, we obtained insight on potential molecular mechanisms of resistance across the countries, finding the kdr mutation V1016I possibly involved in loss of susceptibility.This study is the biggest cross-country update of insecticide resistance for Aedes aegypti in years, and it should be used as evidence for improving the selection of insecticides in these countries and a call for further support to maintain insecticide resistance monitoring programs.

show abstract

The impact of insecticide applications on the dynamics of resistance: The case of four Aedes aegypti populations from different Brazilian regions

Cited by 74 publications

References 46 publications

Patterns of insecticide resistance in Aedes aegypti: meta‐analyses of surveys in Latin America and the Caribbean

Patterns of insecticide resistance in Aedes aegypti: meta‐analyses of surveys in Latin America and the Caribbean

Susceptibility of Aedes aegypti populations to pyriproxyfen in the Federal District of Brazil

The need to harmonize insecticide resistance testing: methodology, intensity concentrations and molecular mechanisms evaluated inAedes aegyptipopulations in Central America and Hispaniola

Contact Info

Product

Resources

About