The impact of l-arginine supplementation on the enteral phase of experimental Trichinella spiralis infection in treated and untreated mice

Evaluation of Clinicopathological alterations in mice experimentally infected with Trichenella spiralis and the Nematocidal effect of Tannic acid and Albendazole

“…The results from the present study are in the same line with Salama et al (2021) who reported an improvement in architecture of intestine in T. spiralis infected mice and treated with albendazole and prednisolone. Additionally, Fadl et al (2020) recorded that the inflammatory cells infiltration and goblet cells were significantly reduced in T. spiralis infected mice which treated by albendazole in comparison to infected non treated mice.…”

“…Life cycle commences with ingestion of raw or under cooked meat that contain the muscle larvae, where the digestive juice in the stomach dissolves the capsulelike cyst which consequently release of the infective larvae that move to the intestine with subsequent penetration to its columnar epithelium (Diaz et al, 2020). Promptly, the larvae differentiate into adults, then, after mating the adult female worms release large number of newborn larvae that penetrate the intestine (Fadl et al, 2020). Next, the larvae convey through the circulatory system into the skeletal muscle cells ISSN (Print) 2786-0272 ISSN (Online) 2786-0280 NVVJ.…”

Investigation of the effect of curcumin on oxidative stress, local inflammatory response, COX-2 expression, and microvessel density in Trichinella spiralis induced enteritis, myositis and myocarditis in mice

“…Different studies have reported different efficacy rates for alben-dazole, according to the dose, duration of administration, timing of animal sacrifice and stage of infection. Fadl et al (2020) examined the effect of albendazole during the intestinal phase of infection. The drug was administered at a dose of 50 mg/kg on day 2 post-infection for 3 days and a reduction rate of 90.91 % was achieved.…”

“…Assessment of adult worm count in small intestine The small intestines of the sacrifi ced mice were removed, opened longitudinally, washed with saline and sectioned into small pieces and incubated at 37°C in phosphate buffered saline (PBS) for 2 hours. Then the adult worms were collected and counted under a dissecting microscope (Fadl et al, 2020) Assessment of larval count in muscle The rectus abdominus muscle of each mouse was dissected and incubated in 1 % pepsin and 1 % HCl in distilled water at 37°C for 2 hours with intermittent agitation using an electric stirrer. Coarse particles were fi rst removed from the digested product on a 50 mesh/inch sieve, then larvae were collected on a 200 mesh/inch sieve, washed twice and suspended in 150 ml tap water in a conical fl ask.…”

Investigation of the effect of the calcium channel blocker, verapamil, on the parasite burden, inflammatory response and angiogenesis in experimental Trichinella spiralis infection in mice

“…The small intestines were opened longitudinally, washed with saline and cut into small pieces. Incubation at 37 °C was done in phosphate buffered saline (PBS) for 2 h. The adult worms were then collected and counted under a dissecting microscope ( Fadl et al, 2020 ).…”